2012 Fiscal Year Final Research Report
Stable-Isotope Probing of plastics film for investigation of surface microbial community
| Project/Area Number |
22350067
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental chemistry
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| Research Institution | University of Tsukuba |
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Principal Investigator |
NAKAJIMA Toshiaki 筑波大学, 生命環境系, 筑波大学・生命環境系・ (80241777)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 生分解性物質 |
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| Research Abstract |
In this study, stable isotope probing of PHB film with 13C in order to explore the PHB degraders from different soil samples. The DNA Extracted from 13C labeled PHB film was subjected to ultracentrifugation. The resultant DNA was found labeled with 13C. It indicates that most of the microbes present on the surface of film might be PHB degraders. This 13C DNA was analyzed through 16S rRNA gene based T-RFLP and clonal analysis to identify different microbial communities. The results indicated the presence of different microbial communities on the surface of PHB films.
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[Journal Article] Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization.2012
Author(s)
Y. Shinozaki, T. Morita, X. H. Cao, S. Yoshida, M. Koitabashi, T. Watanabe, K. Suzuki, Y. Sameshima-Yamashita, T. Nakajima-Kambe, T. Fujii, H. K. Kitamoto
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Journal Title
Appl. Microbiol. Biotechnol.
Peer Reviewed
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[Journal Article] Phyllosphere yeasts rapidly break down biodegradable plastics2011
Author(s)
H. K. Kitamoto, Y. Shinozaki, X. H. Cao, T. Morita, M. Konishi, K. Tago, H. Kajiwara, M. Koitabashi, S. Yoshida, T. Watanabe, Y. Sameshima-Yamashita, T. Nakajima-Kambe, S. Tsushima
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Journal Title
AMB Express
Volume: 1
Pages: 44.
Peer Reviewed
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