2012 Fiscal Year Final Research Report
Development of electrochemical detection methods of biomolecules based on dynamics of biopolymer that immobilized on electrode
| Project/Area Number |
22350072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | University of Toyama |
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Principal Investigator |
INOUYE Masahiko 富山大学, 大学院・医学薬学研究部(薬学), 教授 (60211752)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Hajime 富山大学, 大学院・医学薬学研究部(薬学), 准教授 (10324055)
FUJIMOTO Kazuhisa 九州産業大学, 工学部, 准教授 (40334718)
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| Project Period (FY) |
2010 – 2012
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| Keywords | SNP / SWV / フェロセン / DNA プローブ / ペプチドプローブ / 挿入多型 / 欠損多型 / 電気化学検出 / 全電極 / 電荷移動 |
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| Research Abstract |
Recently, we have reported an electrochemical single nucleotide polymorphism detection by use of the ferrocene-modified DNA probe. In this study, new electrochemical methods based on dynamics of the probe molecules that immobilized on the electrode were developed in order to detect various biomolecules. For example: （1) We established an electrochemical detection method for insertion/deletion polymorphism by tuning the pulse potential frequency in square-wave voltammetry. （2) We developed an electrochemical protein detection method by using the new ferrocene-modified peptide probes.
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[Journal Article] Furanose Ring Conformations in a 1'-Alkynyl C-Nucleoside and the Dinucleotide2012
Author(s)
Chiba, J., Shirato, W., Yamade, Y., Kim, B.-S., Matsumoto, S., and Inouye, M
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Journal Title
Tetrahedron
Volume: 68
Pages: 9045-9049
DOI
Peer Reviewed
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[Journal Article] Electrochemical Direct Detection of DNA Deamination Catalyzed by APOBEC3G2012
Author(s)
Chiba, J., Kouno, T., Aoki, S., Sato, H., Zhang, J.-Y., Matsuo, H., and Inouye, M
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Journal Title
Chem. Commun
Volume: 48
Pages: 12115-12117
DOI
Peer Reviewed
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[Journal Article] Hexamethyldisilazane-Promoted Sonogashira Reaction of Polyfunctionalized N-Containing Heterocycles2011
Author(s)
Inouye, M., Doi, Y., Azuchi, J., Shirato, W., Chiba, J., and Abe, H
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Journal Title
Heterocycles
Volume: 82
Pages: 1137-1141
DOI
Peer Reviewed
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