2012 Fiscal Year Final Research Report
Development of New Methods for Protein Chemical Modification
| Project/Area Number |
22350073
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyushu University |
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Principal Investigator |
OJIDA Akio 九州大学, 薬学研究院, 教授 (10343328)
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| Project Period (FY) |
2010 – 2012
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| Keywords | タンパク質 / 蛍光プローブ、ラベル化 / バイオイメージング / ペプチドデザイン |
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| Research Abstract |
In this research, we have attempted to develop several new chemical methods for protein modification. First, we have developed the covalent protein method using the complementary recognition pair of oligo-His tag and Ni(II)-NTA probe. This labeling method was successfully applied to the labeling of the tag-fused proteins in living cells. Second, we have achieved the design of highly reactive tag-probe pair comprising a-helix type oligo-Asp tag and Zn(II)-DpaTyr probe. This pair showed the higher protein labeling selectivity compared to the previously developed method, validating the our design strategy of the reactive tag-probe pair. Third, we have found the new fluorescent dye for protein modification This fluorescent dye displayed a unique dual-emission change depending on its hydration state, which we expect would be valuable fluorohore to develop protein-based fluorescence biosensors
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