Realtime CARS imaging of cellular response by stimulation of laser ablation

2012 Fiscal Year Final Research Report

• Project/Area Number: 22360027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied optics/Quantum optical engineering
• Research Institution: Osaka University
• Principal Investigator: HASHIMOTO Mamoru 大阪大学, 大学院・基礎工学研究科, 准教授 (70237949)
• Co-Investigator(Kenkyū-buntansha): FUKUSHIMA Shuichiro 大阪大学, 大学院・基礎工学研究科, 助教 (40362644) ; NIIOKA Hirohiko 大阪大学, 大学院・基礎工学研究科, 助教 (70552074)
• Project Period (FY): 2010 – 2012
• Keywords: 応用光学 / 量子光工学

Research Abstract

We have developed a multifocus coherent anti-Stokes Raman scattering microscopy system combined with a laser-beam ablation system. The developed system visualizes sample response by laser stimulation without staining at vide-rate. The laser beam for ablation is controlled independently of observation. The developed system was applied to cellular response by membrane disruption and crystallizationmonitoring by photon pressure.

Research Products

• [Journal Article] Coherent anti-tokes aman scattering microscopy for high speed non-staining biomolecular imaging (2013)
  • Author(s): M. Hashimoto, Minamikawa, and . Araki
  • Journal Title: Curr. Pharm. Biotechnol Volume: vol. 14, No. 2 Pages: 150-158
  • DOI: DOI:10.2174/1389201011314020004
  • Peer Reviewed
• [Journal Article] Real-time imaging of laser-induced membrane disruption of a living cell observed with multi-focus CARS microscopy (2011)
  • Author(s): T. Minamikawa, H. Niioka, T. Araki, and M. Hashimoto
  • Journal Title: J. Biomed. Opt Volume: vol. 16, No. 2 Pages: 021111
  • DOI: DOI:10.1117/1.3533314
  • Peer Reviewed
• [Journal Article] Photo-induced cell damage analysis for multifocus CARS microscopy (2011)
  • Author(s): T. Minamikawa, Y. Murakami, N. Matsumura, H. Niioka,S. Fukushima, T. Araki, and M. Hashimoto
  • Journal Title: Proc.SPIE Volume: vol. 7903 Pages: 79032H
  • DOI: DOI:10.1117/12.874677
  • Peer Reviewed
• [Journal Article] リアルタイムCARS顕微鏡の開発と生細胞観測への応用 (2010)
  • Author(s): 橋本 守, 南川 丈夫, 新岡 宏彦, 荒木勉
  • Journal Title: 日本レーザー医学会誌 Volume: vol. 30No. 4 Pages: 421-426
  • URL: https://www.jstage.jst.go.jp/article/jslsm/30/4/30_4_421/_pdf
  • Peer Reviewed
• [Presentation] Imaging of recrystallized organic molecule by multiplex fourth order coherent Raman microspectroscopy (2013)
  • Author(s): M. Hashimoto, H. Kanoh, H. Niioka, and T. Araki
  • Organizer: Focus on Micrscopy 2013 (FOM2013)
  • Place of Presentation: Maastricht, The Netherlands
  • Year and Date: 20130324-27
• [Presentation] 高速波長走査多焦点CARS顕微鏡による分子振動イメージング"(招待講演) (2012)
  • Author(s): 橋本守,南川丈夫, H. Cahyadi, 新岡宏彦, 荒木勉
  • Organizer: 第35回日本分子生物学会年会 ワークショップ2W4III 「生体分子イメージングへの新たな挑戦～ラマン分光、 プローブレスイメージングによる分子機能解析とがん診断への応用～」
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡, 福岡県
  • Year and Date: 20121211-14
• [Presentation] Detection of lipid uptake by triglyceride deposit cardiomyovasculopathy-indicated fibroblast using CARS microscopy (2012)
  • Author(s): H. Cahyadi, H. Niioka, K. ichi Hirano, C. Ikegami-Nomura, T Araki, and M. Hashimoto
  • Organizer: OSA-JSAP joint symposia, 73th Autumn Meeting
  • Place of Presentation: tha Japan Society of Applied Physics, Ehime University, Ehime
  • Year and Date: 20120911-14
• [Presentation] Fast spectral CARS micorscopy with high-speed tunable picosecond laser detection of lipid metabolism (2012)
  • Author(s): H. Cahyadi, J. Iwatsuka, T Minamikawa, H. Niioka, T. Araki, and M. Hashimoto
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry (ICHC 2012)
  • Place of Presentation: Kyoto
  • Year and Date: 20120826-29
• [Presentation] Fast spectral imaging CARS microscopy with picosecond high speed wavelength scanning laser (2012)
  • Author(s): H. Cahyadi, J. Iwatsuka, T. Minamikawa, H. Niioka, T. Araki, and M. Hashimoto
  • Organizer: Focus on Micrscopy 2012 (FOM2012)
  • Place of Presentation: Singapore
  • Year and Date: 20120401-04
• [Presentation] 多焦点リアルタイムCARS顕微鏡による細胞応答の観測"(招待講演) (2011)
  • Author(s): 橋本守
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形大学, 山形県山形市
  • Year and Date: 20110829-0902
• [Presentation] 多焦点CARS顕微鏡による細胞応答の無染色高速観測"(招待講演) (2010)
  • Author(s): 橋本守
  • Organizer: 日本分光学会 平成22年度 ナノ分光部会・生細胞分光部会合同シンポジウム「分光学が拓く新しいイメージング・イメージングが拓く新しい分光学」
  • Place of Presentation: 東京大学, 東京都
  • Year and Date: 2010-12-10
• [Book] CARS microscopy: Implementation of nonlinear vibrational spectroscopy for far-field and near-field imaging (2012)
  • Author(s): M. Hashimoto, T. Ichimura, and K. Fujita
  • Total Pages: 317-346
  • Publisher: Springer
• [Remarks]
  • URL: http://sml.me.es.osaka-u.ac.jp/araki_lab
• [Patent(Industrial Property Rights)] 波長走査パルス光同期システム (2012)
  • Inventor(s): 橋本守,丸山真幸
  • Industrial Property Rights Holder: 橋本守,丸山真幸
  • Industrial Property Number: 特願2012-001578
  • Filing Date: 2012-01-06
• [Patent(Industrial Property Rights)] パルスレーザ光のタイミング調整装置,調整法及び光学顕微鏡 (2011)
  • Inventor(s): 橋本守,南川丈夫,谷本尚生,小林実,藤田克昌,河田聡,荒木勉
  • Industrial Property Rights Holder: 橋本守,南川丈夫,谷本尚生,小林実,藤田克昌,河田聡,荒木勉
  • Industrial Property Number: 特許第4862164号
  • Acquisition Date: 2011-11-18