2012 Fiscal Year Final Research Report
Studies on development and stabilization of intact liposome biosensor technology for Si-integrated structure
| Project/Area Number |
22360144
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Electron device/Electronic equipment
|
|---|
| Research Institution | Kyoto Institute of Technology |
|---|
Principal Investigator |
NODA Minoru 京都工芸繊維大学, 工芸科学研究科, 教授 (20294168)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIMANOUCHI Toshinori 大阪大学, 基礎工学研究科, 助教 (10335383)
YAMASHITA Kaoru 京都工芸繊維大学, 工芸科学研究科, 准教授 (40263230)
FUKUZAWA Masayuki 京都工芸繊維大学, 工芸科学研究科, 准教授 (60293990)
SOHGAWA Masayuki 大阪大学, 基礎工学研究科, 助教 (70403128)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KUBOI Ryouich 大阪大学, 基礎工学研究科, 名誉教授 (40029567)
UMAKOSHI Hiroshi 大阪大学, 基礎工学研究科, 教授 (20311772)
OKUYAMA Masanori 大阪大学, 基礎工学研究科, 名誉教授 (60029569)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | マイクロ・ナノデバイス / ナノバイオ / バイオデバイス / リポソーム / 固定化 |
|---|
| Research Abstract |
In order to improve and stabilize the precision and performance of liposome biosensor and its sensing technologies, a series of technical considerations and resultant improvements have been done on the sensor as a key component of near future Si-integrated microsensor system. The technical developments are started from immobilization of minute droplet of liposome suspension and intact supramolecule structure. Moreover, new biosensing systems of liposome impedance biosensor and fluorescence array sensor are developed, based on electronics techniques. Finally, the sensitivities and performance of both leakage current sensor and cantilever strain sensor are improved because a resultant quantitative capability of immobilization of minute liposome droplet on the sensor becomes improved. We also note that the impedance sensor can detect the interaction between the liposome and target protein with a 100 nL order. And that the fluorescence array sensor system can discriminate different target biomolecules.
|
