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2013 Fiscal Year Final Research Report

Construction of functional peptide ligands toward peptide biochips

Research Project

  • PDF
Project/Area Number 22360341
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionTokyo Institute of Technology

Principal Investigator

MIHARA Hisakazu  東京工業大学, 生命理工学研究科, 教授 (30183966)

Co-Investigator(Kenkyū-buntansha) TSUTSUMI Hiroshi  東京工業大学, 大学院生命理工学研究科, 助教 (70398105)
Project Period (FY) 2010-04-01 – 2013-03-31
Keywordsバイオチップ / ペプチド / 捕捉剤
Research Abstract

To develop peptide biochips with high-throughput functions, a variety of peptide ligands were constructed by means of chemical and phage-display methods. It is successfully demonstrated that functional peptides were synthesized by a phage-display method using a beta-loop peptide library, peptide-displaying phage-chips were constructed, a variety of sugar-conjugated peptides (with gold-nanoparticles) were prepared, and peptides functioning to cells were also constructed.

  • Research Products

    (12 results)

All 2013 2012 2011 2010

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (5 results) Book (1 results)

  • [Journal Article] A Monosaccharide-modified Peptide Phage Library for Screening of Ligands to Carbohydrate-binding Proteins2013

    • Author(s)
      K. Arai, H. Tsutsumi, H. Mihara
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 23 Pages: 4940-4943

    • Peer Reviewed
  • [Journal Article] Gold Nanoparticles Conjugated with Monosaccharide-modified Peptide for Lectin Detection2012

    • Author(s)
      H. Tsutsumi, H. Ohkusa, H. Park, T.Takahashi, H. Yuasa, H. Mihara
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 22 Pages: 6825-6827

    • Peer Reviewed
  • [Journal Article] A Novel Peptide Array Using a Phage Display System for Protein Detection2011

    • Author(s)
      T. Sawada, K. Ishiguro, T. Takahashi, H. Mihara
    • Journal Title

      Chem. Lett.

      Volume: 40 Pages: 508-509

    • Peer Reviewed
  • [Journal Article] A Novelβ-Loop Scaffold of Phage-Displayed Peptides for Highly Specific Affinities2011

    • Author(s)
      T. Sawada, K. Ishiguro, T. Takahashi, H. Mihara
    • Journal Title

      Mol. BioSyst.

      Volume: 7 Pages: 2558-2562

    • Peer Reviewed
  • [Journal Article] Cell Fingerprint Patterns Using Designedα-Helical Peptides to Screen for Cell-specific Toxicity2011

    • Author(s)
      K. Usui, T. Kakiyama, K.-Y. Tomizaki, M. Mie, E. Kobatake, H. Mihara
    • Journal Title

      Bioorg. Med. Chem. Lett.

      Volume: 21 Pages: 6281-6284

    • Peer Reviewed
  • [Journal Article] Protein-Protein Interaction and Screening : Array-based Techniques for Screening Disease-Associated Biomarkers in Predictive/Early Diagnostics2010

    • Author(s)
      K.-Y. Tomizaki, K. Usui, H. Mihara
    • Journal Title

      FEBS Journal

      Volume: 277 Pages: 1996-2005

    • Peer Reviewed
  • [Presentation] マンノース修飾ペプチドを提示したファージライブラリの構築とコンカナバリンA結合性ペプチドの探索2012

    • Author(s)
      新井佳菜子,大倉裕通,堤浩,三原久和
    • Organizer
      第49回ペプチド討論会
    • Place of Presentation
      鹿児島
    • Year and Date
      20121107-09
  • [Presentation] Designed peptide libraries for cell analyses2012

    • Author(s)
      Hisakazu Mihara
    • Organizer
      2012 International Symposium on Advanced Biological Engineering
    • Place of Presentation
      中国
    • Year and Date
      20121025-29
  • [Presentation] αへリックスペプチドを結合した金ナノ粒子の細胞導入活性とガン細胞応用2011

    • Author(s)
      Park Hyejin,高橋剛,堤浩,三原久和
    • Organizer
      第48回ペプチド討論会
    • Place of Presentation
      札幌
    • Year and Date
      20110927-29
  • [Presentation] レクチンに対する結合多様性を有する単糖導入ペプチド修飾金ナノ粒子の創製2011

    • Author(s)
      大草寛之,朴恵珍,高橋剛,堤浩,湯浅英哉,三原久和
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば
    • Year and Date
      20110912-14
  • [Presentation] Designed peptide libraries for protein and cell analyses2010

    • Author(s)
      Hisakazu Mihara
    • Organizer
      5th International Peptide Symposium
    • Place of Presentation
      京都
    • Year and Date
      20101204-06
  • [Book] デザインペプチドを用いたプロテインアレイと細胞アレイ 化学フロンティア22,生命現象を理解する分子ツール2010

    • Author(s)
      臼井健二,富崎欣也,三原久和
    • Total Pages
      181(79-87)
    • Publisher
      化学同人

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Published: 2015-07-16  

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