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2012 Fiscal Year Final Research Report

Development of basic technology to simplify biocatalytic processes for the production of chemicals

Research Project

  • PDF
Project/Area Number 22360343
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionOsaka University

Principal Investigator

OHTAKE Hisao  大阪大学, 工学研究科, 教授 (10127483)

Co-Investigator(Kenkyū-buntansha) HONDA Khosuke  大阪大学, 工学研究科, 准教授 (90403162)
Project Period (FY) 2010 – 2012
Keywords耐熱性酵素 / 生体触媒 / バイオプロセス / 加熱処理
Research Abstract

We have developed a simple, new bioproduction platform which is potentially applicable to the synthesis of specialty chemicals.The newly developed process has many potential advantages: (i) whatever target metabolites can be specifically produced; (ii) enzyme purification is not required; (iii) no by-product is formed; (iv) bioconversions can be performed using a simple reactor without elaborate process control; and (v) reaction can be prevented from microbial contamination

  • Research Products

    (8 results)

All 2013 2012 2011

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (4 results)

  • [Journal Article] Bioproduction of Vanillin using an Organic Solvent-tolerant2012

    • Author(s)
      N. Wangthamrongwit, K. Klinsakul, A. S. Vangnai, J. Wongkongkatep, P. Inprakhon, K. Honda, H. Ohtake, J. Kato and T. Pongtharangkul
    • Journal Title

      Brevibacillus agri 13. Appl. Microbiol. Biotechnol.

      Volume: 93 Pages: 555-563

    • Peer Reviewed
  • [Journal Article] Construction of membrane-anchoring fusion protein of Thermococcus kodakaraensis KOD1glycerol kinase and its application to repetitive batchwise reactions2012

    • Author(s)
      E. Restiawaty, K. Honda, K. Okano, R. Hirota, T. Omasa, A. Kuroda, H. Ohtake
    • Journal Title

      J. Biosc. Bioeng.

      Volume: 113 Pages: 521-525

    • Peer Reviewed
  • [Journal Article] Synthetic metabolic engineering-A novel, simple technology for designing a chimeric metabolic pathway-2012

    • Author(s)
      Ye, X., Honda, K., Sakai, T., Okano, K., Omasa, T., Hirota , R., Kuroda, A., and Ohtake, H.
    • Journal Title

      Microb. Cell Fact.

      Volume: 11 Pages: 212

    • Peer Reviewed
  • [Journal Article] Analyses of both the alkB gene transcriptional start site and alkB promoter inducing properties in Rhodococcus sp2011

    • Author(s)
      Cappelletti, M., Fedi, S., Frascari, D., Ohtake, H., Turner, R.J., and Zannoni, D
    • Journal Title

      BCP1 as grown on n-alkanes. Appl. Environ. Microbiol.

      Volume: 77 Pages: 1619-1627

    • Peer Reviewed
  • [Presentation] 合成代謝工学による人工ピルビン酸酸化経路の構築2013

    • Author(s)
      本田孝祐、今川貴志、Ye Xiaoting、岡野憲司、大竹久夫
    • Organizer
      日本農芸化学会2013 年度大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-26
  • [Presentation] Synthetic metabolic engineering -A novel, simple technology for designing a chimeric metabolic pathway-2012

    • Author(s)
      本田孝祐、Ye Xiaoting、岡野憲司、大竹久夫
    • Organizer
      Society of Biotechnology, Japan, International Symposium on Green Growth
    • Place of Presentation
      神戸市
    • Year and Date
      2012-10-26
  • [Presentation] Construction of a chimeric glycolysis pathway by synthetic metabolic engineering2012

    • Author(s)
      本田孝祐、Ye Xiaoting、岡野憲司、大竹久夫
    • Organizer
      IBS2012
    • Place of Presentation
      大邱、韓国
    • Year and Date
      2012-09-18
  • [Presentation] Synthetic Metabolic Engineering -the proof of concept2011

    • Author(s)
      本田孝祐、廣田隆一、黒田章夫、大竹久夫
    • Organizer
      International Union of Microbiological Societies 2011 Congress (IUMS 2011)
    • Place of Presentation
      Sapporo.
    • Year and Date
      2011-09-20

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Published: 2014-08-29  

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