2013 Fiscal Year Final Research Report
Screening of ADH gene-specific amplicons from metagenome for enzyme molecular evolution and biocatalysis
| Project/Area Number |
22360349
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Toyama Prefectural University |
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Principal Investigator |
ITOH Nobuya 富山県立大学, 工学部, 教授 (90213066)
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| Co-Investigator(Kenkyū-buntansha) |
牧野 祥嗣 富山県立大学, 工学部, 教授 (20347355)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 生体触媒 / メタゲノム / スクリーニング / 光学活性アルコール / アルコール脱水素酵素 / 進化分子工学 |
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| Research Abstract |
We have developed an efficient PCR-based amplification of diverse alcohol dehydrogenase (adh) genes from soil and compost metagenomes to aid in the synthesis of optically pure alcohols. Forty lsadh-related genes (Hladh) and 30 par-homologous genes (Hpar), were obtained by screening of gene-specific amplicons from metagenome (S-GAM) from the metagenomic library. The amino acids sequences coded by the Hladh genes obtained by this approach were varied (8-99%). The recombinant enzymes obtained were characterized in detail. The results indicated that the enzymes are superior to the known ones to produce chiral alcohols. Thus S-GAM technique offers great biotechnological potential for biocatalysis and enzyme engineering.
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