2013 Fiscal Year Final Research Report
Eco-omics analyse of microbial sulfur cycle in aquatic environments of cryosphere
| Project/Area Number |
22370005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ecology/Environment
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| Research Institution | Hokkaido University |
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Principal Investigator |
FUKUI Manabu 北海道大学, 低温科学研究所, 教授 (60305414)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Hisaya 北海道大学, 低温科学研究所, 助教 (70400009)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 硫黄循環 / メタゲノム / プロテオミクス / 硫酸還元 / 湖沼 / 硫黄酸化 / 機能遺伝子 / 群集構造 |
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| Research Abstract |
Microbial sulfur cycle in aquatic systems of cryosphere is still unclear although the significance of systems linking carbon and nitrogen cycles has been pointed out. Analysis of aprA gene, which encodes a key enzyme in dissimilatory sulfate reduction and sulfur oxidation, showed that newly isolated Sulfuricella denitrificans-like sulfur oxidizer is a key species from geographically distant lakes in Antarctica and Japan. The complete genome sequence analysis indicated their unique sulfur metabolic pathway adapting freshwater environment. The proteomics results suggest that maintenance of proper translation is critical for growth under low-temperature.
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