Study of ecosystem structure and carbon cycling using radiocarbon concentration and molecular level isotope analysis

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ecology/Environment
• Research Institution: Kyoto University
• Principal Investigator: TAYASU Ichiro 京都大学, 生態学研究センター, 准教授 (80353449)
• Co-Investigator(Kenkyū-buntansha): UCHIDA Masao 国立環境研究所, 環境計測研究センター, 主任研究員 (50344289) ; HYODO Fujio 岡山大学, 異分野融合先端研究コア, 助教 (70435535)
• Project Period (FY): 2010 – 2012
• Keywords: 放射性炭素14 / 安定同位体比 / 炭素循環 / 食物網 / 生態系 / アミノ酸 / 河川生態系 / 土壌生態系

Research Abstract

We have done a watershed-level study of the relationship between carbon cycling and ecosystem structure using the measurement of carbon-14 concentrations (Δ^14C value) and nitrogen isotope ratios of individual amino acids. Δ^14C value indicated that carbon source of river food web was both sequestered carbon from watershed and ambient CO_2. The contribution of both pathways depended on the relative importance of algal production (in-stream production) and terrestrial litter input.

Research Products

• [Journal Article] Use of natural abundances of carbon-13 and carbon-14 for stream ecology (2013)
  • Author(s): Ishikawa, N.F., Hyodo, F. and Tayasu, I.
  • Journal Title: Ecological Research Volume: (in press)
  • DOI: DOI:10.1007/s11284-012-1003-z
  • Peer Reviewed
• [Journal Article] Black humic acid dynamics during natural reforestation of Japanese pampas grass (Miscanthus sinensis) (2013)
  • Author(s): Iimura, Y., Fujimoto, M., Tamura, K., Higashi, T., Kondo, M., Uchida, M., Yonebayashi, K. and Fujitake, N
  • Journal Title: Soil Biology and Biochemistry Volume: 57 Pages: 60-67
  • DOI: DOI:10.1016/j.soilbio.2012.07.01
  • Peer Reviewed
• [Journal Article] 石川尚人生物の同位体天然存在比と生物多様性科学 (2013)
  • Author(s): 陀安一郎
  • Journal Title: ぶんせき Pages: 79-85
• [Journal Article] Seasonal and site-specific variability in terrigenous particulate organic carbon concentration in near-shore waters of Lake Biwa (2013)
  • Author(s): Sakai, Y., Karube, Z., Takeyama, T., Kohzu, A., Yoshimizu, C., Nagata, T., Tayasu, I. and Okuda, N
  • Journal Title: Japan. Limnology Volume: 14 Pages: 167-177
  • DOI: DOI:10.1007/s10201-012-0394-4
  • Peer Reviewed
• [Journal Article] Are stored carbohydrates necessary for seed production in temperate deciduous trees? (2013)
  • Author(s): Ichie, T., Igarashi, S., Yoshida, S., Kenzo, T., Masaki, T. and Tayasu, I
  • Journal Title: Journal of Ecology Volume: 101 Pages: 525-531
  • DOI: DOI:10.1111/1365-2745.12038
  • Peer Reviewed
• [Journal Article] Changes in soil aggregate carbon dynamics under no-tillage with respect to earthworm biomass revealed by radiocarbon analysis. (2013)
  • Author(s): Arai, M., Tayasu, I., Komatsuzaki, M., Uchida, M., Shibata, Y. and Kaneko, N
  • Journal Title: Soil & Tillage Research Volume: 126 Pages: 42-49
  • DOI: DOI:10.1016/j.still.2012.07.003
  • Peer Reviewed
• [Journal Article] Contributions of detrital subsidies to aboveground spiders during secondary succession, revealed by radiocarbon and stable isotope signatures (2013)
  • Author(s): Haraguchi, T.F., Uchida, M., Shibata, Y. and Tayasu, I
  • Journal Title: Oecologia Volume: 171 Pages: 935-944
  • DOI: DOI:10.1007/s00442-012-2446-1
  • Peer Reviewed
• [Journal Article] Latitudinal distribution of soil CO_2 efflux and temperature along the Dalton Highway, Alaska (2012)
  • Author(s): Kim Y., Kim S.D., Enomoto H., Kishida K., Kondo M., Uchida M
  • Journal Title: Polar Science Volume: (in press)
  • URL: http://dx.doi.org/10.1016/j.polar.2012.11.002
  • Peer Reviewed
• [Journal Article] Use of radiocarbon to estimate diet ages of earthworms at different climate regions (2012)
  • Author(s): Hyodo, F., Uchida, T., Kaneko, N. and Tayasu, I
  • Journal Title: Applied Soil Ecology Volume: 62 Pages: 178-183
  • DOI: DOI:10.1016/j.apsoil.2012.09.014
  • Peer Reviewed
• [Journal Article] The effect of the feedback cycle between the soil organic carbon and the soil hydrologic and thermal dynamics (2012)
  • Author(s): Mori K., Ise T., Kondo M., Kim Y., Enomoto H. and Uchida, M
  • Journal Title: Open Journal of Ecology Volume: 2 Pages: 90-95
  • Peer Reviewed
• [Journal Article] Dynamics of soil carbon following destruction of tropical rainforest and the subsequent establishment of Imperata grassland in Indonesian Borneo using stable carbon isotopes (2012)
  • Author(s): Yonekura, Y., Ohta, S., Kiyono, Y., Aksa, D., Morisada, K, Tanaka, N. and Tayasu, I.
  • Journal Title: Global Change Biology Volume: 18 Pages: 2606-2616
  • DOI: DOI:10.1111/j.1365-2486.2012.02722.x
  • Peer Reviewed
• [Journal Article] Sticky plant captures prey for symbiotic bug: Is this digestive mutualism? (2012)
  • Author(s): Anderson, B., Kawakita, A. and Tayasu, I
  • Journal Title: Plant Biology Volume: 14 Pages: 888-893
  • DOI: DOI:10.1111/j.1438-8677.2012.00573.x
  • Peer Reviewed
• [Journal Article] Sulfur stable isotope signature identifies the source of reduced sulfur in benthic communities inmacrophyte zones of Lake Biwa, Japan (2012)
  • Author(s): Karube, Z., Okada, N. and Tayasu, I.
  • Journal Title: Limnology Volume: 13 Pages: 269-280
  • DOI: DOI:10.1007/s10201-012-0375-7
  • Peer Reviewed
• [Journal Article] Natural C-14 provides new data for stream food web studies: a comparison with C-13 in multiple stream habitats (2012)
  • Author(s): Ishikawa, N.F., Uchida, M., Shibata, Y. and Tayasu, I
  • Journal Title: Marine and Freshwater Research Volume: 63 Pages: 210-217
  • DOI: DOI:10.1071/MF11141
  • Peer Reviewed
• [Journal Article] Variation in nitrogen isotopic composition in the Selenga river watershed, Mongolia (2012)
  • Author(s): Hyodo, F., Nishikawa, J., Kohzu, A., Fujita, N., Saizen, I., Tsogtbaatar, Javzan, C., Enkhtuya, M., Gantomor, D., Amartuvshin, N., Ishii, R. and Wada, E
  • Journal Title: Limnology Volume: 13 Pages: 155-161
  • DOI: DOI:10.1007/s10201-011-0351-7
  • Peer Reviewed
• [Journal Article] New organic reference materials for carbon- and nitrogen-stable isotope ratio measurements provided by Center for Ecological Research, Kyoto University, and Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology (2011)
  • Author(s): Tayasu, I., Hirasawa, R., Ogawa, N.O., Ohkouchi, N. and Yamada, K
  • Journal Title: Limnology Volume: 12 Pages: 261-266
  • DOI: DOI:10.1007/s10201-011-0345-5
  • Peer Reviewed
• [Journal Article] Evaluation of wastewater nitrogen transformation in a natural wetland (Ulaanbaatar, Mongolia) using dual-isotope analysis of nitrate (2011)
  • Author(s): Itoh, M., Takemon, Y., Makabe, A., Yoshimizu, C., Kohzu, A., Ohte, N., Tumurskh, D., Tayasu, I., Yoshida, N. and Nagata, T
  • Journal Title: Science of the Total Environment Volume: 409 Pages: 1530-1538
  • DOI: DOI:10.1016/j.scitotenv.2011.01.019
  • Peer Reviewed
• [Presentation] 森林施業が河川生態系へ及ぼす長期的影響とその解明:安定同位体比を利用して (2013)
  • Author(s): 冨樫博幸・加藤義和・石川尚人・吉村真由美・徳地直子・大手信人・由水千景・陀安一郎
  • Organizer: 第124回日本森林学会大会
  • Place of Presentation: 岩手大学
  • Year and Date: 2013-03-27
• [Presentation] アミノ酸窒素同位体比を用いた、陸域分解系における土壌動物の資源利用解析 (2013)
  • Author(s): 陀安一郎・長谷川尚志・由水千景・力石嘉人・小川奈々子・大河内直彦
  • Organizer: 日本生態学会第60回大会.グランシップ
  • Place of Presentation: 静岡
  • Year and Date: 2013-03-07
• [Presentation] 植生二次遷移過程における、クモ群集及びエサ供給量の変化と同位体食物網の関係 (2013)
  • Author(s): 原口岳・陀安一郎
  • Organizer: 日本生態学会第60回大会.グランシップ
  • Place of Presentation: 静岡
  • Year and Date: 2013-03-07
• [Presentation] ミカン畑と魚類をつなぐ硝酸:土地利用が河川生態系へ及ぼす影響に関する安定同位体解析 (2013)
  • Author(s): 冨樫博幸・加藤義和・石川尚人・尾坂兼一・吉村真由美・由水千景・徳地直子・陀安一郎・大手信人
  • Organizer: 日本生態学会第60回大会.グランシップ
  • Place of Presentation: 静岡
  • Year and Date: 2013-03-07
• [Presentation] アミノ酸の窒素安定同位体比を用いた琵琶湖産魚類の栄養段階推定 (2013)
  • Author(s): 加藤義和・石川尚人・冨樫博幸・由水千景・陀安一郎・奥田昇
  • Organizer: 日本生態学会第60回大会.グランシップ
  • Place of Presentation: 静岡
  • Year and Date: 2013-03-07
• [Presentation] 河川複雑系食物網の高精度解析～アミノ酸窒素安定同位体比からのアプローチ～ (2013)
  • Author(s): 石川尚人・加藤義和・冨樫博幸・吉村真由美・由水千景・奥田昇・陀安一郎
  • Organizer: 日本生態学会第60回大会.グランシップ
  • Place of Presentation: 静岡
  • Year and Date: 2013-03-07
• [Presentation] 同位体生態学の進展と生物多様性研究 (2013)
  • Author(s): 陀安一郎
  • Organizer: 第2回同位体環境学シンポジウム(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2013-02-19
• [Presentation] The effect of the feedback cycle between the soil organic carbon and the soil hydrologic and thermal dynamics (2013)
  • Author(s): Mori K., Ise T., Kondo M., Kim Y., Enomoto, H. and Uchida, M
  • Organizer: Third International symposium on the Arctic Research
  • Place of Presentation: 日本科学未来館,東京
  • Year and Date: 2013-01-16
• [Presentation] Estimation of Carbon loss by fire in a burned black spruce forest in interior Alaska using radiocarbon (2012)
  • Author(s): Kondo, M., Uchida, M., Shibata, Y., Iwata,H. and Harazono, Y
  • Organizer: AGU Fall meeting 2012
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-04
• [Presentation] 琵琶湖沿岸生態系の生物多様性に影響を及ぼす駆動因 (2012)
  • Author(s): 柴田淳也・苅部甚一・酒井陽一郎・武山智博・陀安一郎・佐藤祐一・谷内茂雄・中野伸一・奥田昇
  • Organizer: 日本陸水学会第77回大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-09-16
• [Presentation] アミノ酸の窒素安定同位体比から読み解く山地渓流域の食物網構造一護摩壇山試験地の事例一 (2012)
  • Author(s): 冨樫博幸・加藤義和・石川尚人・吉村真由美・徳地直子・大手信人・由水千景・陀安一郎
  • Organizer: 日本陸水学会第77回大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-09-16
• [Presentation] アミノ酸の窒素安定同位体比を用いた琵琶湖流域の食物網解析 (2012)
  • Author(s): 加藤義和・石川尚人・冨樫博幸・吉村真由美・由水千景・奥田昇・陀安一郎
  • Organizer: 日本陸水学会第77回大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-09-16
• [Presentation] 放射性炭素14天然存在比を用いた陸水生態系の解析手法 (2012)
  • Author(s): 石川尚人・内田昌男・柴田康行・陀安一郎
  • Organizer: 日本陸水学会第77回大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2012-09-16
• [Presentation] 植生二次遷移過程におけるクモ群集変化の特徴 (2012)
  • Author(s): 原口岳・陀安一郎
  • Organizer: 日本蜘蛛学会第44回大会
  • Place of Presentation: 山形市保健センター
  • Year and Date: 2012-08-25
• [Presentation] Radiocarbon based organic source identification of soil CO_2 under soil warming in a cultivated Andisol (2012)
  • Author(s): Kondo, M., Uchida, M., Kishimoto, A., Yonemura, S., Wagai, R., Jomura, M. and Shibata, Y
  • Organizer: 21st International Radiocarbon conference
  • Place of Presentation: Paris France
  • Year and Date: 2012-07-13
• [Presentation] Multiple isotope approach for studying material flow and food web structures of stream ecosystems in Lake Biwa watershed. (2012)
  • Author(s): Tayasu, I., Okuda N., Tokuchi N., Ohte N., and Kondoh M
  • Organizer: ASLO Summer Meeting
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2012-07-12
• [Presentation] Biological specimens tell us a centurial history of ecosystem alterations in the ancient Lake Biwa. (2012)
  • Author(s): Okuda, N., Kato, Y., Komiya, T., Okuzaki, Y., Hori, M., Tayasu, I., and Nagata, T
  • Organizer: ASLO Summer Meeting
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2012-07-12
• [Presentation] Alteration of nitrogen isotopic composition during microbial decomposition of suspended particulate organic matter (2012)
  • Author(s): Yoshimizu, C. and Tayasu I
  • Organizer: ASLO Summer Meeting
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2012-07-12
• [Presentation] A difference in food web structures with various forest stand ages in headwater streams in central japan (2012)
  • Author(s): Togashi, H., Kato, Y., Ishikawa, N. F., Yoshimura, M., Tokuchi, N., Ohte, N., Okuda, N. and Tayasu, I.
  • Organizer: ASLO Summer Meeting
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2012-07-12
• [Presentation] Fate of atmospheric nitrate and nitrogen biogeochemical processes in lake Biwa elucidated by multiple oxygen isotopes (2012)
  • Author(s): Makabe, A., Koba, K., Yoshimizu, C., Tayasu, I., Ogawa, N. O., Ohkouchi, N., Toyoda, S., Yoshida, N. and Nagata, T
  • Organizer: ASLO Summer Meetin
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2012-07-12
• [Presentation] Evidence of old carbon subsidization to stream food webs approached by C-14 natural abundance method (2012)
  • Author(s): Ishikawa N., Uchida, M., Shibata, Y. and Tayasu I.
  • Organizer: ASLO Summer Meeting
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2012-07-11
• [Presentation] Food web analysis based on δ^15N of amino acid in river ecosystem (2012)
  • Author(s): Kato, Y., Ishikawa, N.F., Togashi, H., Yoshimura, M., Itoh, M., Osaka, K., Okuda, N., Ohte, N. Yoshimizu, C. and Tayasu I.
  • Organizer: ASLO Summer Meeting
  • Place of Presentation: 滋賀県大津市
  • Year and Date: 2012-07-11
• [Presentation] 山地渓 流域の食物連鎖長:決定機構に果たす生 産性の役割 (2012)
  • Author(s): 冨樫博幸・徳地直子・陀安一郎
  • Organizer: 日本生態学会第59回大会
  • Place of Presentation: 龍谷大学
  • Year and Date: 2012-03-20
• [Presentation] 森林の遷移段階に ともなうアリ群集の食性変化 (2012)
  • Author(s): 田中洋・兵藤不二夫
  • Organizer: 日本生態学 会第59回大会
  • Place of Presentation: 龍谷大学
  • Year and Date: 2012-03-19
• [Presentation] High-throughput measurement for stable isotope analysis of dissolved organic carbon, using wet chemical oxidation. (2011)
  • Author(s): Itoh, M., Tayasu, I., Yoshimizu, C. and Ohte, N.
  • Organizer: AGU Fall Meeting.
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2011-12-06
• [Presentation] 溶存有機炭素の炭素安定同位体比のハイスループットオンライン分析手法の開発 (2011)
  • Author(s): 伊藤雅之・陀安一郎・由水千景・大手信人
  • Organizer: 日本陸水学会第76回大会
  • Place of Presentation: 島根大学
  • Year and Date: 2011-09-23
• [Presentation] 流域地質が河川食物網を流れる炭素の14C年代に及ぼす影響 (2011)
  • Author(s): 石川尚人・内田昌男・柴田康行・陀安一郎
  • Organizer: 日本陸水学会第76回大会
  • Place of Presentation: 島根大学
  • Year and Date: 2011-09-23
• [Presentation] Natural carbon-14 signature provides new data for stream food web studies. (2011)
  • Author(s): Ishikawa N., Uchida, M., Shibata, Y. and Tayasu, I.
  • Organizer: 96th ESA Annual Meeting
  • Place of Presentation: Austin, USA
  • Year and Date: 2011-08-09
• [Book] 安定同位体比を用いた高次生産者の栄養段階の推定
  • Author(s): 陀安一郎
  • Total Pages: 290
  • Publisher: 大学学術出版会
• [Book] 淡水生態学のフロンティア
  • Author(s): 陀安一郎・苅部甚一・石川尚人
  • Total Pages: 269
  • Publisher: 共立出版