Elucidating the fundamental questions "photochemical reaction and its site" using unique cyanobacteria

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370017
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: TSUCHIYA Tohru 京都大学, 大学院・人間・環境学研究科, 准教授 (20362569)
• Co-Investigator(Kenkyū-buntansha): AKIMOTO Seiji 神戸大学, 分子フォトサイエンス研究センター, 准教授 (40250477) ; TOMO Tatsuya 東京理科大学, 理学部, 准教授 (60300886)
• Project Period (FY): 2010 – 2012
• Keywords: 光誘起電子移動反応 / 酸化還元電位 / 超高速分光

Research Abstract

We studied the photochemical reaction of photosystems that convert light energy into chemical energy and thylakoid membranes formation as fundamental questions. Photosystem II complexes isolated from a chlorophyll d-dominated cyanobacterium that can utilized near infra-red light for photochemical reaction unlike plantswere analyzed. The results revealed the bioenergetics of photosystems driven by lower energy than those from plants.

Research Products

• [Journal Article] Excitation energy relaxation in a symbiotic cyanobacterium, Prochloron didemni, occurring in coral-reef ascidians, and in a free-living cyanobacterium,Prochlorothrix hollandica. (2012)
  • Author(s): Hamada, F., Yokono, M., Hirose, E., Murakami, A., Akimoto, S
  • Journal Title: Biochim. Biophys. Acta Volume: 1817 Pages: 1992-1997
  • DOI: doi:10.1016/j.bbabio.2012.06.008
  • Peer Reviewed
• [Journal Article] Artificially produced [7-formyl]-chlorophyll dfunctions as an antenna pigment in the photosystem II isolated from the chlorophyllide aoxygenase-expressing Acaryochloris marina (2012)
  • Author(s): Tsuchiya, T., Akimoto, S., Mizoguchi, T., Watabe, K., Kindo, H., Tomo, T., Tamiaki, H., Mimuro, M.
  • Journal Title: Biochim. Biophys. Acta Volume: 1817 Pages: 1285-1291
  • DOI: doi:10.1016/j.bbabio.2012.02.021
  • Peer Reviewed
• [Journal Article] Metabolic engineering of the chlorophyll d-dominated cyanobacterium Acaryochloris marina: production of a novel chlorophyll species by the introduction of the chlorophyllide aoxygenase gene (2012)
  • Author(s): Tsuchiya, T., Mizoguchi, T., Akimoto, S., Tomo, T., Tamiaki, H., Mimuro, M
  • Journal Title: Plant Cell Physiol. Volume: 53 Pages: 518-527
  • DOI: doi:10.1093/pcp/pcs007
  • Peer Reviewed
• [Journal Article] Luminescence of singlet oxygen in photosystem II complexes isolated from cyanobacterium Synechocystissp. PCC6803 containing monovinyl or divinyl chlorophyll a (2012)
  • Author(s): Tomo, T., Kusakabe, H., Nagao, R., Ito, H., Tanaka, A., Akimoto, S., Mimuro, M., Okazaki, S
  • Journal Title: Biochim. Biophys. Acta Volume: 1817 Pages: 1299-1305
  • DOI: doi:10.1016/j.bbabio.2012.02.018
  • Peer Reviewed
• [Journal Article] Alterations in photosynthetic pigments and amino acid composition of D1 protein change energy distribution in photosystem II (2012)
  • Author(s): Yokono, M., Tomo, T., Nagao, R., Ito, H., Tanaka, A., Akimoto, S
  • Journal Title: Biochim. Biophys. Acta Volume: 1817 Pages: 754-759
  • DOI: doi:10.1016/j.bbabio.2012.02.009
  • Peer Reviewed
• [Journal Article] Redox potentials of primary electron acceptor quinone molecule (Q_A)-and conserved energetics of photosystem II in cyanobacteria with chlorophyll aand chlorophyll d. (2011)
  • Author(s): Allakhverdiev, S.I., Tsuchiya, T., Watabe, K., Kojima, A., Los, D.A., Tomo, T., Klimov, V.V., Mimuro, M
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Pages: 8054-8058
  • DOI: doi:10.1073/pnas.1100173108
  • Peer Reviewed
• [Journal Article] Molecular environments of divinyl chlorophylls in Prochlorococcusand Synechocystis: Differences in fluorescence properties with chlorophyll replacement (2011)
  • Author(s): Mimuro, M., Murakami, A., Tomo, T., Tsuchiya, T., Watabe, K., Yokono, M., Akimoto, S
  • Journal Title: Biochim. Biophys. Acta Volume: 1807 Pages: 471-481
  • DOI: doi:10.1016/j.bbabio.2011.02.011
  • Peer Reviewed
• [Journal Article] Structural coupling of an arginine side chain with the oxygen evolving Mn4Ca cluster in photosynstem II as revealed by isotope-edited fourier transform infrared spectroscopy. (2011)
  • Author(s): Shimada, Y., Suzuki, H., Tsuchiya, T., Mimuro, M., Noguch, T
  • Journal Title: J. Am. Chem. Soc Volume: 133 Pages: 3808-3811
  • DOI: doi:10.1021/ja200186h
  • Peer Reviewed
• [Journal Article] Redox potential of pheophytin ain photosystem II of two cyanobacteria having the different special pair chlorophylls (2010)
  • Author(s): Allakhverdiev, S.I., Tomo, T., Shimada, Y., Kindo, H., Nagao, R., Klimov, V.V., Mimuro, M
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 3924-3929
  • DOI: doi:10.1073/pnas.0913460107
  • Peer Reviewed
• [Presentation] 代謝工学的アプローチから探る光化学系IIの色素組成の柔軟性 (2013)
  • Author(s): 土屋 徹
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学(岡山)
  • Year and Date: 20130321-23
• [Presentation] クロロフィルdを主要色素としてもつシアノバクテリアの光化学系II 反応機構 (2013)
  • Author(s): 鞆 達也
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学(岡山)
  • Year and Date: 20130321-23
• [Presentation] Application of time-resolved fluorescence spectroscopy to studies on light adaptation of photosynthetic organisms (2013)
  • Author(s): Akimoto, S.
  • Organizer: Transdisciplinary cooperation and application of nanoscience
  • Place of Presentation: Krakow, Poland
  • Year and Date: 2013-02-22
• [Presentation] 人工的に合成した[7-ホルミル]-クロロフィルdを含む光化学系IIの色素組成 (2012)
  • Author(s): 土屋 徹、秋本 誠志、渡部 和幸、鞆 達也、三室 守
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 20120316-18
• [Presentation] Acaryochloris marinaから光化学系II反応中心の単離・精製とその性質 (2012)
  • Author(s): 遠藤 嘉一郎、鈴木 健裕、長尾 遼、堂前 直、鞆 達也
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 20120316-18
• [Presentation] Amino acid modifications improved excitation energy distribution in pigment-altered photosystem II reaction center. (2011)
  • Author(s): Yokono, M., Tomo, T., Nagao, R., Ito, H., Tanaka, A., Akimoto, S
  • Organizer: 5th Asia and Oceania Conference for Photobiology
  • Place of Presentation: Nara Prefectural New Public Hall, Nara
  • Year and Date: 20110730-0801
• [Presentation] 新奇クロロフィルを合成するAcaryochloris marinaMBIC 11017の形質転換体からの光化学系の単離とその性質 (2011)
  • Author(s): 土屋 徹、秋本 誠志、金籐 隼人、鞆 達也、三室 守
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 20110320-22
• [Presentation] Acaryochloris marinaの光化学系II色素組成 (2011)
  • Author(s): 小島 茜、長尾 遼、三室 守、鞆 達也
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 20110320-22