ChloroplastSignalsregulatingplantimmuneresponses

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto Prefectural University
• Principal Investigator: SHIINA Takashi 京都府立大学, 大学院・生命環境科学研究科, 教授 (10206039)
• Co-Investigator(Kenkyū-buntansha): YOSHIOKA Hirofumi 名古屋大学, 大学院・生命農学研究科, 准教授 (30240245)
• Project Period (FY): 2010 – 2012
• Keywords: 植物分子機能 / 葉緑体 / Ca2+ / サリチル酸 / 感染防御 / 逆行性シグナル

Research Abstract

Salicylic acid acts as an endogenous signal that triggers local and systemic response in the plant immune system. Both reactive oxygen species (ROS) and nitric oxide (NO) may play important roles in regulation of immune cell signal transductions. It should be noted that chloroplasts are involved in the production of theses immune signals. However, a role for chloroplasts in plant immune system remains largely elusive. This study provides novel insights into chloroplastic control of plant immunity through a thylakoid-localized Ca2+ binding protein CAS.

Research Products

• [Journal Article] Chloroplast envelope localization of EDS5, an essential factor for salicylic acid biosynthesis in Arabidopsis thaliana. (2013)
  • Author(s): Yamasaki K, Motomura Y, Yagi Y, Nomura H, Kikuchi S, Nakai M, Shiina T.
  • Journal Title: Plant Signal Behav. Volume: 18
  • DOI: DOI:pii: e23603.
  • Peer Reviewed
• [Journal Article] Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis. (2013)
  • Author(s): Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH.
  • Journal Title: Plant J. Volume: 2013 73 Pages: 761-75
  • DOI: DOI:10.1111/tpj.12069.
  • Peer Reviewed
• [Journal Article] The Variable Domain of a Plant Calcium-dependent Protein Kinase (CDPK) Confers Subcellular Localization and Substrate Recognition for NADPH Oxidase. (2013)
  • Author(s): Asai S, Ichikawa T, Nomura H, Kobayashi M, Kamiyoshihara Y, Mori H, Kadota Y, Zipfel C, Jones JD, Yoshioka H.
  • Journal Title: J Biol Chem. Volume: 288 Pages: 14332-40
  • DOI: DOI:10.1074/jbc.M112.448910.
  • Peer Reviewed
• [Journal Article] Eukaryotic-type plastid nucleoid protein pTAC3 is essential for transcription by the bacterial-type plastid RNA polymerase. (2012)
  • Author(s): Yagi Y, Ishizaki Y, Nakahira Y, Tozawa Y, Shiina T.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 109 Pages: 7541-6
  • DOI: DOI:10.1073/pnas.1119403109.
  • Peer Reviewed
• [Journal Article] Chloroplast-mediated activation of plant immune signalling in Arabidopsis. (2012)
  • Author(s): Nomura H, Komori T, Uemura S, Kanda Y, Shimotani K, Nakai K, Furuichi T, Takebayashi K, Sugimoto T, Sano S, Suwastika IN, Fukusaki E, Yoshioka H, Nakahira Y, Shiina T.
  • Journal Title: Nat Commun.26 Volume: 3 Pages: 926
  • DOI: DOI:10.1038/ncomms1926.
  • Peer Reviewed
• [Journal Article] Defense response of a pepper cultivar cv. Sy-2 is induced at temperatures below 24°C. (2012)
  • Author(s): Koeda S, Hosokawa M, Kang BC, Tanaka C, Choi D, Sano S, Shiina T, Doi M, Yazawa S.
  • Journal Title: J Plant Res. Volume: 125 Pages: 137-45
  • URL: http://link.springer.com/article/10.1007%2Fs10265-011-0414-1
  • Peer Reviewed
• [Journal Article] Functional characterization of ObgC in ribosome biogenesis during chloroplast development. (2012)
  • Author(s): Bang WY, Chen J, Jeong IS, Kim SW, Kim CW, Jung HS, Lee KH, Kweon HS, Yoko I, Shiina T, Bahk JD.
  • Journal Title: Plant J. Volume: 71 Pages: 122-34
  • DOI: DOI:10.1111/j.1365-313X.2012.04976.x.
  • Peer Reviewed
• [Journal Article] Evolutionary aspects of plastid proteins involved in transcription: The transcription of a tiny genome is mediated by a complicated machinery. (2012)
  • Author(s): Yagi Y, Shiina T.
  • Journal Title: Transcription. Volume: 3 Pages: 1-5
  • URL: http://www.landesbioscience.com/journals/transcription/article/21810/?nocache=1659960848
  • Peer Reviewed
• [Journal Article] StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. (2012)
  • Author(s): Nomura H, Kuchimura K, Mori H, Doke N, Yoshioka H.
  • Journal Title: New Phytol. Volume: 196 Pages: 223-37
  • DOI: DOI:10.1111/j.1469-8137.2012.04226.x.
  • Peer Reviewed
• [Journal Article] Use of ultra-performance liquid chromatography/time-of-flight mass spectrometry with nozzle-skimmer fragmentation for comprehensive quantitative analysis of secondary metabolites in Arabidopsis thaliana. (2011)
  • Author(s): Sugimoto T, Bamba T, Izumi Y, Nomura H, Shiina T, Fukusaki E.
  • Journal Title: J Sep Sci. Volume: 34 Pages: 3587-96
  • DOI: DOI:10.1002/jssc.201100552.
  • Peer Reviewed
• [Journal Article] Nuclear/nucleolar GTPase 2 proteins as a subfamily of YlqF/YawG GTPases function in pre-60S ribosomal subunit maturation of mono- and dicotyledonous plants. (2011)
  • Author(s): Im CH, Hwang SM, Son YS, Heo JB, Bang WY, Suwastika IN, Shiina T, Bahk JD.
  • Journal Title: J Biol Chem. Volume: 286 Pages: 8620-3
  • DOI: DOI:10.1074/jbc.M110.200816.
  • Peer Reviewed
• [Journal Article] Selective excitation of photosystems in chloroplasts inside plant leaves observed by near-infrared laser-based fluorescence spectral microscopy. (2010)
  • Author(s): Hasegawa M, Shiina T, Terazima M, Kumazaki S.
  • Journal Title: Plant Cell Physiol. Volume: 51 Pages: 225-38
  • DOI: DOI:10.1093/pcp/pcp182.
  • Peer Reviewed
• [Presentation] Chloroplast Ca2+ and Plant Immunity (2013)
  • Author(s): Takashi Shiina
  • Organizer: 3rd International Symposium on Chloroplast Genomics and Genetic Engineering
  • Year and Date: 20130500
• [Presentation] 植物の細胞内シグナル伝達系と共生オルガネラ (2013)
  • Author(s): 椎名隆
  • Organizer: 第54回植物生理学会(シンポジウム)
  • Year and Date: 20130300
• [Presentation] (2013)
  • Author(s): Takashi Shiina
  • Organizer: 29th IPSR International Symposium and 5th Symposium on Plant Sciences
  • Year and Date: 20130200
• [Presentation] 野村裕也葉緑体Ca2+シグナリングと植物のストレス・感染防御応答 (2011)
  • Author(s): 椎名隆
  • Organizer: 日本植物学会第75回大会(シンポジウム)
  • Year and Date: 20110900
• [Presentation] 野村裕也植物免疫応答におけるCa2+シグナルとCAS の役割 (2010)
  • Author(s): 椎名隆
  • Organizer: 日本植物学会第74回大会(シンポジウム)
  • Year and Date: 20100900