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2012 Fiscal Year Final Research Report

Diversification of ciliary structure and its mechanism during evolution of body plan

Research Project

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Project/Area Number 22370023
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphology/Structure
Research InstitutionUniversity of Tsukuba

Principal Investigator

INABA Kazuo  筑波大学, 生命環境系, 教授 (80221779)

Co-Investigator(Renkei-kenkyūsha) YAGUCHI Shunsuke  筑波大学, 生命環境系, 准教授 (00505331)
SHIBA Kogiku  筑波大学, 生命環境系, 助教 (70533561)
DEGAWA Yosuke  筑波大学, 生命環境系, 助教 (00311431)
Project Period (FY) 2010 – 2012
Keywords微細構造
Research Abstract

This research was carried out to analyze the diversification of ciliary structure during evolution and showed following new aspects;
(1) Roles of glutathion transferase theta in the mechanical reception and signal transduction of apical tuft in sea urchin embryos.
(2) Array structure of axonemes in the parasperm of a sea snail.
(3) Driving of chemotaxis by a novel calcium sensor calaxin in ascidian sperm.
(4) Evolutional acquisition of calaxin in the group of opisthokont.

  • Research Products

    (49 results)

All 2012 2011 2010 Other

All Journal Article (13 results) (of which Peer Reviewed: 11 results) Presentation (33 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Black Tea HighMolecular-Weight Polyphenol Increases the Motility of Sea Urchin Sperm by Activating Mitochondrial Respiration.2012

    • Author(s)
      Kikuchi A, Shiba K, Ozawa T, Nakano K, Inaba K, Numata O
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 76(12) Pages: 2321-4

    • Peer Reviewed
  • [Journal Article] Calaxin drives sperm chemotaxis by Ca2+-mediated direct modulation of a dynein motor2012

    • Author(s)
      Mizuno K, Shiba K, Okai M, Takahashi Y, Shitaka Y, Oiwa K, Tanokura M, Inaba K
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 109(50) Pages: 20497-502

    • Peer Reviewed
  • [Journal Article] Self-incompatibility response induced by calcium increase in sperm of the ascidian Ciona intestinalis2012

    • Author(s)
      Saito T, Shiba K, Inaba K, Yamada L, Sawada H
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 109(11) Pages: 4158-62

    • Peer Reviewed
  • [Journal Article] Tubulin-dynein system in flagellar and ciliary movement2012

    • Author(s)
      Mohri H, Inaba K, Ishijima S, Baba SA
    • Journal Title

      Proc Jpn Acad Ser B Phys Biol Sci

      Volume: 88(8) Pages: 397-415

    • Peer Reviewed
  • [Journal Article] Isolation and functional characterization for oocyte maturation and sperm motility of the oocyte maturation arresting factor from the Japanese scallop, Patinopecten yessoensis2012

    • Author(s)
      Yuan Y, Tanabe T, Maekawa F, Inaba K,Maeda Y, Itoh N, Takahashi KG, Osada M
    • Journal Title

      Gen Comp Endocrinol

      Volume: 179(3) Pages: 350-7

    • DOI

      doi:10.1016/j.ygcen.2012.09.006

    • Peer Reviewed
  • [Journal Article] Zinc finger homeobox is required for the differentiation of serotonergic neurons in the sea urchin embryo2012

    • Author(s)
      Yaguchi J, Angerer LM, Inaba K, Yaguchi S
    • Journal Title

      Dev Biol

      Volume: 363(1) Pages: 74-83

    • DOI

      doi:10.1016/j.ydbio.2011.12.024

    • Peer Reviewed
  • [Journal Article] Fez function is required to maintain the size of the animal plate in the sea urchin embryo2011

    • Author(s)
      Yaguchi S, Yaguchi J, Wei Z, Jin Y, Angerer LM, Inaba K
    • Journal Title

      Development

      Volume: 138(19) Pages: 4233-43

    • Peer Reviewed
  • [Journal Article] Sperm flagella: comparative and phylogenetic perspectives of protein components.2011

    • Author(s)
      Inaba K
    • Journal Title

      Mol Hum Reprod

      Volume: 17(8) Pages: 524-38

    • DOI

      doi:10.1093/molehr/gar034

    • Peer Reviewed
  • [Journal Article] Proteomic profiling reveals compartment-specific, novel functions of ascidian sperm proteins.2011

    • Author(s)
      Nakachi M, Nakajima A, Nomura M, Yonezawa K, Ueno K, Endo T, Inaba K
    • Journal Title

      Mol Reprod Dev

      Volume: 78(7) Pages: 529-49

  • [Journal Article] CIPRO 2.5: Ciona intestinalis protein database, a unique integrated repository of large-scale omics data, bioinformatic analyses and curated annotation, with user rating and reviewing functionality.2011

    • Author(s)
      Endo T, Ueno K, Yonezawa K, Mineta K, Hotta K, Satou Y, Yamada L, Ogasawara M, Takahashi H, Nakajima A, Nakachi M, Nomura M, Yaguchi J, Sasakura Y, Yamasaki C, Sera M, Yoshizawa AC, Imanishi T, Taniguchi H, Inaba K
    • Journal Title

      Nucleic Acids Res

      Volume: 39(Database issue):D807-14

  • [Journal Article] Lipid rafts function in Ca2+signaling responsible for activation of sperm motility and chemotaxis in the ascidian Ciona intestinalis.2011

    • Author(s)
      Zhu L, Inaba K
    • Journal Title

      Mol Reprod Dev

    • DOI

      doi:10.1002/mrd.21382

    • Peer Reviewed
  • [Journal Article] an k AT-1 is a novel gene mediating the apical tuft formation in the sea urchin embryo.2010

    • Author(s)
      Yaguchi S, Yaguchi J, Wei Z, Shiba K, Angerer LM, Inaba K
    • Journal Title

      Dev Biol

      Volume: 348(1) Pages: 67-75

    • DOI

      doi:10.1016/j.ydbio.2010.09.011

    • Peer Reviewed
  • [Journal Article] Network structure of projections extending from peripheral neurons in the tunic of ascidian larva2010

    • Author(s)
      Terakubo HQ, Nakajima Y, Sasakura Y, Horie T, Konno A, Takahashi H, Inaba K, Hotta K, Oka K
    • Journal Title

      Dev Dyn

      Volume: 239(8) Pages: 2278-87

    • DOI

      doi:10.1002/dvdy.22361

    • Peer Reviewed
  • [Presentation] Adenylyl cyclases and Ca2+ signaling involved in sperm flagellar motility activation in C.intestinalis2012

    • Author(s)
      Kogiku Shiba, Kazuo Inaba
    • Organizer
      The International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      Hotel Nagoya Garden Palace, Nagoya, Japan
    • Year and Date
      20121112-16
  • [Presentation] Mechanism of Ca2+-mediated signaling for sperm motility2012

    • Author(s)
      Kazuo Inaba
    • Organizer
      The International Symposium on the Mechanisms of Sexual Reproduction in Animals and Plants
    • Place of Presentation
      Hotel Nagoya Garden Palace, Nagoya, Japan
    • Year and Date
      20121112-16
  • [Presentation] ウニ胚頂毛におけるグルタチオン S-トランスフェラーゼthetaの機能2012

    • Author(s)
      金銀華,谷口順子,柴小菊,谷口俊介,稲葉一男
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学,大阪
    • Year and Date
      20120913-15
  • [Presentation] マガキガイStrombus luhuanus の異型精子形成メカニズム2012

    • Author(s)
      柴田大輔,稲葉一男
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学,大阪
    • Year and Date
      20120913-15
  • [Presentation] マガキガイ正型精子のカルシウム依存的な後方遊泳2012

    • Author(s)
      柴小菊,柴田大輔,稲葉一男
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学,大阪
    • Year and Date
      20120913-15
  • [Presentation] A Neuronal Calcium Sensor Protein, Calaxin, is Essential for Modulation of Ciliary Beat Direction in Sea Urchin Embryos2011

    • Author(s)
      K. Mizuno, J. Yaguchi, K. Shiba, S. Yaguchi, K. Inaba
    • Organizer
      2011 The American Society for Cell Biology annual meeting
    • Place of Presentation
      Denver, USA
    • Year and Date
      20111203-07
  • [Presentation] LC-MS/MSによるウニ胚頂毛特異的タンパク質の解析2011

    • Author(s)
      稲葉一男
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      20110921-23
  • [Presentation] マガキガイStrombus luhuanusの異型精子形成2011

    • Author(s)
      柴田大輔,稲葉一男
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      20110921-23
  • [Presentation] ウニ胚繊毛運動におけるCa2+結合タンパク質カラクシンの機能2011

    • Author(s)
      水野克俊,谷口順子,柴小菊,谷口俊介,稲葉一男
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      20110921-23
  • [Presentation] Tunicate databases: Toward a comprehensive informative basis at molecular and cellular level for tunicate community2011

    • Author(s)
      Inaba K
    • Organizer
      The 6th International Tunicate Meeting
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20110703-07
  • [Presentation] Lipid rafts function in Ca2+/calpain-dependent degradation of Ci-Sp17 in the signaling for activation of sperm motility in the ascidian Ciona intestinalis2011

    • Author(s)
      Lihong Zhu, Akiko Hozumi, Lixy Yamada, Hitoshi Sawada, Kazuo Inaba
    • Organizer
      The 6th International Tunicate Meeting
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20110703-07
  • [Presentation] Self-incompatibility response induces intracellular Ca2+ influx into sperm in Ciona intestinalis2011

    • Author(s)
      Takako Saito, Kogiku Shiba, Kazuo Inaba, Lixy Yamada, Hitoshi Sawada
    • Organizer
      The 6th International Tunicate Meeting
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20110703-07
  • [Presentation] CIPRO 2.5: Ciona intestinalis protein database, a unique integrated repository of large-scale omics data, bioinformatic analyses and curated annotation, with user rating and reviewing functionality.2011

    • Author(s)
      Toshinori Endo, Keisuke Ueno, Kouki Yonezawa, Katsuhiko Mineta, Kohji Hotta, Yutaka Satou, Lixy Yamada, Michio Ogasawara, Hiroki Takahashi, Ayako Nakajima, Mia Nakachi, Mamoru Nomura, Junko Yaguchi, Yasunori Sasakura, Chisato Yamazaki, Miho Sera, Akiyasu
    • Organizer
      The 6th International Tunicate Meeting
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20110703-07
  • [Presentation] The function of Ca2+ in the regulation of sperm flagellar motility2011

    • Author(s)
      Inaba K
    • Organizer
      The 17th International Symposium on Ca2+-Binding Proteins and Ca2+ Function in Health and Disease
    • Place of Presentation
      Beijing, China
    • Year and Date
      20110700
  • [Presentation] Ca2+結合タンパク質カラクシンは外腕ダイニンを調節し精子走化性に必須である.2011

    • Author(s)
      水野克俊,柴小菊,稲葉一男
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      札幌
    • Year and Date
      20110627-29
  • [Presentation] Proteomic approach to study the functional diversity of cilia and flagella in marine invertebrates2011

    • Author(s)
      Inaba K
    • Organizer
      1st World Congress on Marine Biotechnology
    • Place of Presentation
      Dalian, China
    • Year and Date
      20110400
  • [Presentation] Analysis of the molecular architecture and the microstructure of the apical tuft in the sea urchin embryos2011

    • Author(s)
      Jin Y, Yaguchi S, Nakajima Y, Konno A,Inaba K
    • Organizer
      The Developmental Biology of the Sea Urchin XX
    • Place of Presentation
      Woods Hole
    • Year and Date
      20110400
  • [Presentation] Formation and motility of parasperm in the gastropod Strombus luhuanus2011

    • Author(s)
      D.Shibata, K.Inaba
    • Organizer
      6th Asian-Pacific Organization for Cell Biology Congress
    • Place of Presentation
      EDSA Shangri-la, Manila, Philippines
    • Year and Date
      20110200
  • [Presentation] Proteomics, cell biology and phsiology of sperm flagella2011

    • Author(s)
      Inaba K
    • Organizer
      6th Asian-Pacific Organization for Cell Biology Congress
    • Place of Presentation
      Manila, Philippines
    • Year and Date
      20110200
  • [Presentation] Lipid Rafts Is Involved in Ca2+/Calpain-Dependent Degradation of Ci-Sp17 for Activation of Sperm Motility in the Ascidian Ciona Intestinalis2010

    • Author(s)
      L. Zhu, A. Hozumi, K. Inaba
    • Organizer
      2010 The American Society for Cell Biology annual meeting
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      20101211-15
  • [Presentation] ウニ精子軸糸への外腕ダイニン重鎖の再結合2010

    • Author(s)
      毛利秀雄,久保・入江美代子,入江克,稲葉一男
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京
    • Year and Date
      20100923-25
  • [Presentation] ウニ胚頂毛に存在するグルタチオン S-トランスフェラーゼの胚繊毛運動における役割2010

    • Author(s)
      金銀華,柴小菊,谷口俊介, 稲葉一男
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京
    • Year and Date
      20100923-25
  • [Presentation] NCSファミリータンパク質カラクシンのホヤ精子走化性における機能2010

    • Author(s)
      水野克俊,柴小菊,稲葉一男
    • Organizer
      日本動物学会第81回大
    • Place of Presentation
      東京
    • Year and Date
      20100923-25
  • [Presentation] NCS family protein calaxin is a calcium dependent dynein modulator responsible for sperm chemotaxis.2010

    • Author(s)
      Katsutoshi Mizuno, Kogiku Shiba, Kazuo Inaba
    • Organizer
      FASEB Summer Research Confer ences: The Biology of Cilia and Flagella
    • Place of Presentation
      Vermont, USA
    • Year and Date
      20100705-10
  • [Presentation] Characterization of adenylylcyclase involved in flagellar motility activation in Ciona sperm.2010

    • Author(s)
      Kogiku Shiba, Akiko Hozumi, Kazuo Inaba
    • Organizer
      FASEB Summer Research Confer ences: The Biology of Cilia and Flagella
    • Place of Presentation
      Vermont, USA
    • Year and Date
      20100705-10
  • [Presentation] Comparative proteomics of cilia and flagella reveals distinctive regulation of sperm outer dynein.2010

    • Author(s)
      Alu Konno, Kazuo Inaba
    • Organizer
      FASEB Summer Research Confer ences: The Biology of Cilia and Flagella.
    • Place of Presentation
      Vermont, USA
    • Year and Date
      20100705-10
  • [Presentation] Recent topics in the architecture and function of sperm flagella.2010

    • Author(s)
      Kazuo Inaba
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      20100624-29
  • [Presentation] In situ assembly of heavy chains of the outer arm dynein in sea urchin sperm axoneme2010

    • Author(s)
      Miyoko Kubo-Irie,Masaru Irie,Kazuo Inaba,Hideo Mohri
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      20100624-29
  • [Presentation] Characterization of adenylylcyclase involved in sperm motility activation in Ciona intestinalis2010

    • Author(s)
      Kogiku Shiba, Akiko Hozumi, Kazuo Inaba
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      20100624-29
  • [Presentation] Function of NCS family protein calaxin in the regulation of sperm flagellar waveform2010

    • Author(s)
      Katsutoshi Mizuno, Kogiku Shiba, Kazuo Inaba
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      20100624-29
  • [Presentation] Sperm Proteomics in an Ascidian Ciona intestinalis with Spatial and Functional Information2010

    • Author(s)
      Mia Nakachi,Ayako Nakajima,Mamoru Nomura,Akiko Hozumi,Kouki Yonezawa,Keisuke Ueno,Toshinori Endo,Kazuo Inaba
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      20100624-29
  • [Presentation] Sperm Lipid Membrane Domain Is Essential for the Calpain-Dependent Degradation of a Cancer-Testis Antigen Sp17 during Ascidian Sperm Activation2010

    • Author(s)
      Lihong Zhu,Akiko Hozumi,Kazuo Inaba
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      20100624-29
  • [Presentation] Proteomic characterization of sperm radial spokes identifies a novel spoke protein with an ubiquitin domain2010

    • Author(s)
      Yuhkoh Satouh,Kazuo Inaba
    • Organizer
      11th International Symposium on Spermatology
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      20100624-29
  • [Book] Regulatory subunits of axonemal dynein. Handbook of Dynein2012

    • Author(s)
      Inaba K
    • Total Pages
      303-324
    • Publisher
      PanStanford Publishing
  • [Book] Spermatology: new horizons in the 21 century2012

    • Author(s)
      Shiba K, Mizuno K and Inaba K
    • Total Pages
      30-40
    • Publisher
      Adthree Publishing Co. Ltd
  • [Remarks] ホームページ

    • URL

      http://www.shimoda.tsukuba.ac.jp/~inaba/

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Published: 2014-08-29  

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