2013 Fiscal Year Final Research Report
The study for the mechanism of steroid hormone-dependent sex determination in amphibians
| Project/Area Number |
22370027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphology/Structure
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| Research Institution | Waseda University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
松田 洋一 (70165835)
長谷部 孝 (70329027)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 組織構築 / アンドロゲン / アンドロゲン受容体 / トランスゲニックカエル / 性転換 / ツチガエル |
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| Research Abstract |
Sex of an amphibian species Rana rugosa can be changed from female to male or in the opposite direction by treatment of sex steroid hormones. To induce the sex-reversal, androgens must bind its receptor. The androgen receptor (AR) gene is localized to the Z and W chromosome. The AR gene on the Z chromosome (Z-AR) is expressed normally, but W-AR is barely expressed, suggesting that this gene is probably involved in male sex determination in R. rugosa frogs. To prove this hypothesis, we produced transgenic frogs carrying an exogenous Z-AR. AR-transgenic female (ZW) frogs formed masculinized gonad or 'ovotestis'. Testosterone, supplied to the rearing water, completed the female-to-male sex-reversal in the AR-transgenic ZW frogs. This is the first report showing that the AR gene plays a very important role in male sex-determination in a vertebrate species.
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Research Products
(12 results)
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[Journal Article] Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish2011
Author(s)
N Suzuki, J.A. Danks, Y. Maruyama, M. Ikegame, Y. Sasayama, A. Hattori, M. Nakamura, M.J. Tabata, T. Yamamoto, R. Furuya, K. Saijoh, H. Mishima, A.K. Srivastav, Y. Furusawa, T. Kondo, Y. Tabuchi, I. Takasaki, V.S. Chowdhury, K. Hayakawa, T.J. Martin
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Journal Title
Bone
Volume: 48
Pages: 1186-1193
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