2012 Fiscal Year Final Research Report
Time-resolved structure analysis of type-II restriction endonuclease HindIII
| Project/Area Number |
22370036
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
WATANABE Nobuhisa 名古屋大学, シンクロトロン光研究センター, 教授 (70212321)
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| Project Period (FY) |
2010 – 2012
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| Keywords | X線 / 結晶構造解析 / 制限酵素 / 反応機構 / 時間分割 / フリーズトラップ |
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| Research Abstract |
We have performed the time-resolved X-ray crystal structure analysis of a type-II restriction endonuclease HindIII using the freeze trap method. Crystals of HindIII and its cognate DNA complex were soaked in a solution containing metal ion, and flash-freezed to stop its reaction after the elapse of a certain period of time. In the reaction, two metal ions are necessary, and the scission reaction takes place after binding of the second metal ion.
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