Structural study on substrate recognition mechanism of the S2P-type I-CLiP

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370039
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama City University (2011-2012); Osaka University (2010)
• Principal Investigator: NOGI Terukazu 横浜市立大学, 大学院・生命ナノシステム科学研究科, 准教授 (40379102)
• Project Period (FY): 2010 – 2012
• Keywords: 立体構造解析 / 膜タンパク質 / プロテアーゼ / 結晶化 / シグナル伝達

Research Abstract

In this project, we carried out structural and biochemical studies on bacterial intramembrane proteases so as to elucidate the molecular mechanism of substrate recognition in the regulated intramembrane proteolysis (RIP). We successfully determined the crystal structure of the periplasmic domain of the intramembrane protease. In addition, we found that the domain is involved in the substrate discrimination in the course of RIP.

Research Products

• [Journal Article] Higher-order architecture of cell adhesion mediated by polymorphic synaptic adhesion molecules neurexin and neuroligin (2012)
  • Author(s): Tanaka, H., Miyazaki, N., Matoba, K., Nogi, T., Iwasaki, K. and Takagi, J
  • Journal Title: Cell Rep Volume: 2 Pages: 101-110
  • DOI: DOI:10.1016/j.celrep.2012.06.009.
  • Peer Reviewed
• [Journal Article] structure of the ligand binding domain of α5β1 integrin: Atomic details of the fibronectin receptor. (2012)
  • Author(s): Nagae, M., Re, S., Mihara, E., Nogi, T., Sugita, Y., Takagi, J.Crystal
  • Journal Title: J. Cell Biol Volume: 197 Pages: 131-140
  • DOI: DOI:10.1083/jcb.201111077
  • Peer Reviewed
• [Journal Article] Structural basis for variant-specific neuroligin-binding by α-neurexin (2011)
  • Author(s): Tanaka, H., Nogi, T., Yasui, N., Iwasaki, K. and Takagi, J
  • Journal Title: PLoS ONE Volume: 6 Pages: e19411
  • DOI: DOI:10.1371/journal.pone.0019411
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary crystallographic analysis of human LR11 Vps10p domain (2010)
  • Author(s): Nakata, Z., Nagae, M., Yasui, N., Bujo, H., Nogi, T. and Takagi, J
  • Journal Title: Acta Cryst Volume: F67 Pages: 129-132
  • DOI: DOI:10.1107/S1744309110048153
  • Peer Reviewed
• [Journal Article] Structural basis for semaphorin signalling through the plexin receptor (2010)
  • Author(s): Nogi, T., Yasui, N., Mihara, E., Matsunaga, Y., Noda, M., Yamashita, N., Toyofuku, T., Uchiyama, S., Goshima, Y., Kumanogoh, A. and Takagi, J
  • Journal Title: Nature Volume: 467 Pages: 1123-1127
  • DOI: DOI:10.1038/nature09473
  • Peer Reviewed
• [Presentation] 大腸菌RIPプロテアーゼRsePのタンデムPDZドメインによる新たな機能制御メカニズム (2012)
  • Author(s): 檜作洋平、禾晃和、田畑早苗、川上-田村恵子、小田隆、佐藤衛、高木淳一、秋山芳展
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2012-12-16
• [Presentation] 膜内配列切断(RIP)におけるPDZドメインを介した"基質認識" (2012)
  • Author(s): 禾晃和
  • Organizer: 大阪大学蛋白質研究所セミナー・包括脳ネットワーク研究会、第3回神経科学と構造生物学の融合研究会(招待講演)
  • Place of Presentation: 大阪大学蛋白質研究所(吹田市)
  • Year and Date: 2012-10-04
• [Presentation] 大腸菌表層ストレス応答に関与する膜内切断プロテアーゼRsePのPDZドメインによる新たな機能制御メカニズム (2012)
  • Author(s): 檜作洋平、禾晃和、田畑早苗、川上-田村恵子、小田隆、佐藤衛、高木淳一、秋山芳展
  • Organizer: 第6回細菌学若手コロッセウム
  • Place of Presentation: 大学セミナーハウス(八王子市)
  • Year and Date: 2012-08-10
• [Presentation] 膜内切断プロテアーゼRsePの基質取り込み制御におけるPDZタンデムの役割 (2012)
  • Author(s): 禾晃和、檜作洋平、田畑早苗、川上-田村恵子、小田隆、佐藤衛、高木淳一、秋山芳展
  • Organizer: 第12回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2012-06-21
• [Presentation] 、Structural analysis of PDZ modules of bacterial site-2 protease (2010)
  • Author(s): 禾晃和、高 木 淳 一
  • Organizer: 26th European Crystallographic Meeting
  • Place of Presentation: 、Darmstadtium Conference Center (ドイツ、ダルムシュタット市)
  • Year and Date: 2010-09-01
• [Remarks] Terukazu NOGI / Yokohama City University
  • URL: http://www-mls.tsurumi.yokohama-cu.ac.jp/xtal-mls/members/nogi/index.html