2012 Fiscal Year Final Research Report
Mass Spectrometry of Metalloproteins and High-Molecular-Weight Protein Complexes
| Project/Area Number |
22370040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OKUMURA Nobuaki 大阪大学, たんぱく質研究所, 准教授 (20224173)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 蛋白質複合体 / 金属蛋白質 / エレクトロスプレーイオン化質量分析 / ネイティブ状態 / 金属イオン / 化学量論 |
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| Research Abstract |
A method for high-efficient ion transmission of high-m/z molecular ions has been developed and embedded in a conventional electrospray ionization mass spectrometer. The method allows for measurement of large molecular complexes such as a protein complex, metalloprotein, etc. in native states, which is useful for revealingstoichiometry of molecules that form a complex or metal ions that are coordinated in a metalloprotein.
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[Journal Article] Significance of AlanineAminopeptidase N (APN) in Bile in the Diagnosis of Acute Cellular Rejection After Liver Transplantation2012
Author(s)
Kim C, Aono S, Marubashi S, Wada H, Kobayashi S, Eguchi H, Takeda Y, Tanemura M, Okumura N, Takao T, Doki Y, Mori M, Nagano H.
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Journal Title
J Surg Res
Volume: 175
Pages: 138-148
Peer Reviewed
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[Journal Article] Charge state-selective separation of peptides by reversible modification of amino groups and strong cation-exchange chromatography: evaluation in proteomic studies using peptide-centric database searches.2011
Author(s)
Betancourt LH, Sanchez A, Perez Y, Fernandez de Cossio J, Gil J, Toledo P, Iguchi S, Aimoto S, Gonzalez LJ, Padron G, Takao T, Besada V.
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Journal Title
J. Proteomics.
Volume: 74
Pages: 2210-2213
Peer Reviewed
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[Journal Article] Expression of C4.4A at the invasive front is a novel prognostic marker for disease recurrence of colorectal cancer2010
Author(s)
Konishi K, Yamamoto H, Mimori K, Takemasa I, Mizushima T, Ikeda M, Sekimoto, M, Matsuura N, Takao T, Doki Y, Mori M.
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Journal Title
Cancer Sci.
Volume: 101(10)
Pages: 2269-2277
Peer Reviewed
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