Analysis of self-renewal mechanisms of embryonic stem cells

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370050
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kanazawa University
• Principal Investigator: YOKOTA Takashi 金沢大学, 医学系, 教授 (50134622)
• Co-Investigator(Kenkyū-buntansha): KOIDE Hiroshi 金沢大学, 医学系, 准教授 (70260536) ; AKAGI Tadayuki 金沢大学, 医学系, 助教 (70532183)
• Project Period (FY): 2010 – 2012
• Keywords: 細胞情報伝達機構 / 未分化性維持機構

Research Abstract

Mouse embryonic stem (ES) cells have two major characteristics: self-renewal capacity and pluripotency. Here, we focused on Eed (epigenetic controlling factor), Baf53a (chromatin remodeler), LRH-1 (cell cycle regulator), and transcription factor network (relationship among Oct3/4, Dax1 and Esrrb; and Gli). From the investigations of these factors, we revealed molecular mechanisms of self-renewal capacity of ES cells.

Research Products

• [Journal Article] Dax1 Associates with Esrrb and Regulates Its Function in Embryonic Stem Cells (2013)
  • Author(s): Uranishi K, Akagi T, Sun C, Koide H, Yokota T
  • Journal Title: Mol Cell Biol Volume: 33 Pages: 2056-66
  • DOI: DOI:10.1128/MCB.01520-12
  • Peer Reviewed
• [Journal Article] Inecalcitol, an analog of 1α,25(OH)(2) D(3) , induces growth arrest of androgen-dependent prostate cancer cells (2012)
  • Author(s): Okamoto R, Delansorne R, Wakimoto N, Doan NB, Akagi T, Shen M, Ho QH, Said JW, Koeffler HP
  • Journal Title: Int J Cancer Volume: 130 Pages: 2464-73
  • DOI: DOI:10.1002/ijc.26279
  • Peer Reviewed
• [Journal Article] RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3 (2012)
  • Author(s): Tavares L, Dimitrova E, Oxley D, Webster J, Poot R, Demmers J, Bezstarosti K, Taylor S, Ura H, Koide H, Wutz A, Vidal M, Elderkin S, Brockdorff N
  • Journal Title: Cell Volume: 148 Pages: 664-78
  • DOI: DOI:10.1016/j.cell.2011.12.029.
  • Peer Reviewed
• [Journal Article] β-catenin enhances Oct-4 activity and reinforces pluripotency through a TCF-independent mechanism (2011)
  • Author(s): Kelly KF, Ng DY, Jayakumaran G, Wood GA, Koide H, Doble BW
  • Journal Title: Cell Stem Cell Volume: 8 Pages: 214-27
  • DOI: DOI:10.1016/j.stem.2010.12.010
  • Peer Reviewed
• [Journal Article] Eed /Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation (2011)
  • Author(s): Ura H, Murakami K, Akagi T, Kinoshita K, Yamaguchi S, Masui S, Niwa H, Koide H, Yokota T
  • Journal Title: EMBO J Volume: 30 Pages: 2190-204
  • DOI: DOI:10.1038/emboj.2011.126
  • Peer Reviewed
• [Journal Article] マウスES細胞の自己複製維持におけるDax1とEsrrbの相互作用 (2011)
  • Author(s): 浦西洸介
  • Journal Title: 金沢大学十全医学会雑誌 Volume: 第120巻第2号 Pages: 60-61
• [Journal Article] In vivo deficiency of both C/EBPβ and C/EBPε results in highly defective myeloid differentiation and lack of cytokine response (2010)
  • Author(s): Akagi T, Thoennissen NH, George A, Crooks G, Song JH, Okamoto R, Nowak D, Gombart AF, Koeffler HP
  • Journal Title: PLoS One Volume: 5 Pages: e15419
  • DOI: DOI:10.1371/journal.pone.0015419
  • Peer Reviewed
• [Journal Article] A stem cell-derived gene (Sddr) negatively regulates differentiation of embryonic stem cells (2010)
  • Author(s): Miura M, Ueda A, Takao Y, Nishimura EK, Koide H, Yokota T
  • Journal Title: Int J Dev Biol Volume: 54 Pages: 33-9
  • DOI: DOI:10.1387/ijdb.082802mm
  • Peer Reviewed
• [Journal Article] Genomic profiling of adult acute lymphoblastic leukemia by single nucleotide polymorphism oligonucleotide microarray and comparison to pediatric acute lymphoblastic leukemia (2010)
  • Author(s): Okamoto R, Ogawa S, Nowak D, Kawamata N, Akagi T, Kato M, Sanada M, Weiss T, Haferlach C, Dugas M, Ruckert C, Haferlach T, Koeffler HP
  • Journal Title: Haematologica. Volume: 95 Pages: 1481-8
  • DOI: DOI:10.3324/haematol.2009.011114.
  • Peer Reviewed
• [Journal Article] 正常核型急性骨髄性白血病のSNP アレイ解析 (2010)
  • Author(s): 赤木紀之
  • Journal Title: 血液フロンティア(医薬ジャーナル社) Volume: 20 Pages: 31-37
  • URL: https://www.iyaku-j.com/iyakuj/system/M2-1/summary_viewer.php?trgid=22011
• [Presentation] マウスES細胞におけるLRH-1の増殖制御機構の解析 (2012)
  • Author(s): 金井大,武石健,上田篤,赤木紀之,小出寛,横田崇
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡県・福岡国際会議場
  • Year and Date: 20121214-16
• [Presentation] Zfp296のマウスES細胞における役割 (2012)
  • Author(s): 藤井優佳,懸川まどか,赤木紀之,小出寛,横田崇
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡県・福岡国際会議場
  • Year and Date: 20121211-14
• [Presentation] INVOLVEMENT OF ETS-RELATED TRANSCRIPTION FACTORS ETV4 AND ETV5 IN PLURIPOTENCY AND PROLIFERATION OF MOUSE EMBRYONIC STEM CELLS (2012)
  • Author(s): Tadayuki Akagi, Satu Kuure, Hiroshi Koide, Frank Costantini, Takashi Yokota
  • Organizer: ISSCR 10th Annual Meeting
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 20120613-16
• [Presentation] DAX1 ASSOCIATES WITH ESRRB AND FUNCTIONS AS A REPRESSOR IN EMBRYONIC STEM CELLS (2012)
  • Author(s): Kousuke Uranishi, Tadayuki Akagi, Chuanhai Sun, Hiroshi Koide, and Takashi Yokota
  • Organizer: I SSCR 10th Annual Meeting
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 20120613-16
• [Presentation] NANOG/ZFP57 PATHWAY PROMOTES ANCHORAGE INDEPENDENT GROWTH OF HT1080 CELLS BY INDUCING IMPRINTED GENES EXPRESSION (2012)
  • Author(s): Tada Yuhki, Tadayuki Akagi, Takashi Yokota, and Hiroshi Koide
  • Organizer: I SSCR 10th Annual Meeting
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 20120613-16
• [Presentation] Ets-related transcription factors ETV4/5 are involved in pluripotency and proliferation of mouse ES cells (2011)
  • Author(s): Tadayuki Akagi,Satu Kuure,Hiroshi Koide,Frank Costantini,Takashi Yokota
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 20111213-16
• [Presentation] マウスES細胞の自己複製維持におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西 洸介,赤木 紀之,孫 伝海,小出 寛,横田 崇
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 20111213-16
• [Presentation] 癌細胞におけるES 細胞特異的遺伝子Zfp57の機能解析 (2011)
  • Author(s): 田多 祐喜,赤木 紀之,横田 崇,小出 寛
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 神奈川県・パシフィコ横浜
  • Year and Date: 20111213-16
• [Presentation] Ets-related transcription factors ETV4 and ETV5 are involved in pluripotency and proliferation of mouse embryonic stem cells (2011)
  • Author(s): Tadayuki Akagi, Satu Kuure, Hiroshi Koide, Frank Costantini, Takashi Yokota
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
  • Year and Date: 20110525-26
• [Presentation] Eed and Sox2 form regulatory loop that regulates ES cell self-renewal by modulating histon methylation and acetylation (2011)
  • Author(s): Hiroki Ura, Kazuhiro Murakami, Tadayuki Akagi, Keita Kinoshita, Shukuro Yamaguchi, Shinji Masui, Hitoshi Niwa, Hiroshi Koide, Takashi Yokota
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
  • Year and Date: 20110525-26
• [Presentation] マウスES細胞におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西 洸介、赤木 紀之、孫 伝海、小出 寛、横田 崇
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
  • Year and Date: 20110525-26
• [Presentation] Nanog regulates anchorage-independent growth of HT1080 fibrosarcoma cells (2011)
  • Author(s): Yuhki Tada, Tadayuki Akagi, Takashi Yokota, Hiroshi Koide
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
  • Year and Date: 20110525-26
• [Presentation] ヒト繊維芽肉腫HT1080 細胞の増殖におけるPDGF-Cの関与 (2011)
  • Author(s): 金城 智章, 横田 崇, 小出 寛
  • Organizer: 日本分子生物学会第11回春季シンポジウム
  • Place of Presentation: 石川県・石川県立音楽堂
  • Year and Date: 20110525-26
• [Presentation] Involvement of nuclear hormone receptors Dax1 and Esrrb in the maintenance of self-renewal in ES cells (2011)
  • Author(s): Kousuke Uranishi, Tadayuki Akagi, Chuanhai Sun, Hiroshi Koide, Takashi Yokota
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 東京都・泉ガーデンギャラリー
  • Year and Date: 20110513-14
• [Presentation] Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation (2011)
  • Author(s): Hiroshi Koide, Hiroki Ura, Kazuhiro Murakami, Tadayuki Akagi, Keita Kinoshita, Shukuro Yamaguchi, Shinji Masui, Hitoshi Niwa, Takashi Yokota
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 東京都・泉ガーデンギャラリー
  • Year and Date: 20110513-14
• [Presentation] anog regulates anchorage-independent growth of HT1080 fibrosarcoma cells (2011)
  • Author(s): Yuhki Tada, Tadayuki Akagi, Takashi Yokota, Hiroshi Koide
  • Organizer: The 9th Stem Cell Research Symposium
  • Place of Presentation: 東京都・泉ガーデンギャラリー
  • Year and Date: 20110513-14
• [Presentation] 未分化なマウスES細胞におけるOct3/4、Dax1、Esrrbの相互作用 (2011)
  • Author(s): 浦西 洸介、赤木 紀之、孫 伝海、小出 寛、横田 崇
  • Organizer: 日本生化学会北陸支部第29回大会
  • Place of Presentation: 石川県・金沢大学
  • Year and Date: 2011-05-28
• [Presentation] Nanogはヒト繊維肉腫HT1080細胞の足場非依存性増殖に必要である (2011)
  • Author(s): 田多 祐喜, 赤木 紀之, 横田 崇, 小出寛
  • Organizer: 日本生化学会北陸支部第29回大会
  • Place of Presentation: 石川県・金沢大学
  • Year and Date: 2011-05-28
• [Presentation] 未分化なES細胞特異的に発現しているDax1 とEsrrb の相互作用 (2010)
  • Author(s): 浦西 洸介,赤木 紀之,孫 伝海,小出 寛,横田 崇
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 20101207-10
• [Presentation] Nanog is essential for anchorage-independent growth of HT1080 fibrosarcoma cells (2010)
  • Author(s): 田多 祐喜,赤木 紀之,横田 崇,小出寛
  • Organizer: BMB2010
  • Place of Presentation: 兵庫県・神戸ポートアイランド
  • Year and Date: 20101207-10
• [Presentation] マウスES細胞の分化にはEedの適切な発現レベルが必要である (2010)
  • Author(s): 小出 寛、横田 崇
  • Organizer: 第31回日本炎症・再生医学会
  • Place of Presentation: 東京都・京王プラザホテル
  • Year and Date: 20100805-06
• [Presentation] マウ スES細胞の自己複製や分化にはEedの適切な発現レベルが必要である (2010)
  • Author(s): 小出 寛
  • Organizer: 十全医学会学術集会
  • Place of Presentation: 石川県・金沢大学十全講堂
  • Year and Date: 2010-07-09
• [Presentation] ES細胞におけるGli2の発現制御機構の解析 (2010)
  • Author(s): 上田 篤、田多 祐喜、浦 大樹、赤木 紀之、小出 寛、横田 崇
  • Organizer: 日本生化学会北陸支部第28回大会
  • Place of Presentation: 福井県・福井大学
  • Year and Date: 2010-05-29
• [Presentation] ヒト繊維肉腫HT1080細胞の増殖機構におけるNanogの役割 (2010)
  • Author(s): 田多 祐喜、赤木 紀之、 横田 崇、 小出寛
  • Organizer: 日本生化学会北陸支部第28回大会
  • Place of Presentation: 福井県・福井大学
  • Year and Date: 2010-05-29
• [Book] The LIF/STAT3 Pathway in ES Cell Self-renewal in Embryonic Stem Cells. The Hormonal Regulation of Pluripotency and Embryogenesis (2011)
  • Author(s): Hiroshi Koide and Takashi Yokota
  • Total Pages: 61-78
  • Publisher: Craig S. Atwood, ed.
• [Book] Vitamin D and Hematologic Malignancies (Chapter 11) In: "Vitamin D and Cancer (1st edition) (2010)
  • Author(s): Okamoto R, Akagi T, Koeffler HP
  • Total Pages: 251-278
  • Publisher: (Donald L. Trump and Candace S. Johnson eds)
• [Remarks] ホームページ
  • URL: http://medstem.w3.kanazawa-u.ac.jp
• [Remarks] Atsushi Ueda. Involvement of Gli proteins in undifferentiated state maintenance and proliferation of embryonic stem cells. 金沢大学十全医学会雑誌 第121巻2号38-46(2012)
• [Remarks] 浦西洸介、マウスES細胞の自己複製維持におけるDax1とEsrrbの相互作用、金沢大学十全医学会雑誌 第120巻 第2号 60-61(2011)