Molecular mechanism and physiological role of FLASH with pleiotropic activities

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370052
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Kyoto University
• Principal Investigator: YONEHARA Shin 京都大学, 大学院・生命科学研究科, 教授 (00124503)
• Project Period (FY): 2010 – 2012
• Keywords: 細胞周期 / 転写 / 細胞分化 / ES細胞

Research Abstract

FLASH has been shown to be required for replication-dependent histone transcription and S phase progression. We showed that FLASH is involved in transcriptional regulation through modulating histone methylation by directly interacting to histone lysine-specific demethylase 1 (LSD1) and corepressor of REST 1 (CoREST1) in the CoREST1 complex. We also showed that induced knockout of FLASH does not affect proliferation of diploid fibroblasts and ES cells, and FLASH was shown to be dispensable for ES cells to differentiate into mesoderm, endoderm, and trophoblast. FLASH might be required for cell-cycle progression specifically in transformed cells

Research Products

• [Journal Article] Fas deficiency in mice with the Balb/c background induces blepharitis with allergic inflammation and hyper-IgE production in conjunction with severe autoimmune disease (2013)
  • Author(s): Takahashi S, Futatsugi-Yumikura S, Fukuoka A, Yoshimoto T, Nakanishi K, and Yonehara S.
  • Journal Title: Int Immunol Volume: 25 Pages: 287-293
  • Peer Reviewed
• [Journal Article] Fas-mediated inflammatory response in Listeria monocytogenes infection (2013)
  • Author(s): Uchiyama R, Yonehara S, and Tsutsui H
  • Journal Title: J Immunol Volume: 190 Pages: 4245-4254
  • Peer Reviewed
• [Journal Article] Dclk1 distinguishes between tumor and normal stem cells in the intestine (2013)
  • Author(s): Nakanishi Y, Seno H, Fukuoka A, Ueo T, Yamaga Y, Maruno T, Nakanishi N, Kanda K, Komekado H, Kawada M, Isomura A, Kawada K, Sakai Y, Yanagita M, Kageyama R, Kawaguchi Y, Taketo MM, Yonehara S, and Chiba T.
  • Journal Title: Nat Genet Volume: 45 Pages: 98-103
  • Peer Reviewed
• [Journal Article] Protease activity of procaspase-8 is essential for cell survival by inhibiting both apoptotic and nonapoptotic cell death dependent on receptor interacting protein kinase 1 (RIP1) and RIP3 (2012)
  • Author(s): Kikuchi M, Kuroki S, Kayama M, Sakaguchi S, Lee KK and Yonehara S
  • Journal Title: J Boil Chem Volume: 287 Pages: 41165-41173
  • Peer Reviewed
• [Journal Article] Identification of a mechanism that couples multisite phosphorylation of Yes-Associated Protein (YAP) with transcriptional coactivation and regulation of apoptosis (2012)
  • Author(s): Lee KK and Yonehara S
  • Journal Title: J Biol Chem Volume: 287 Pages: 9568-9578
  • Peer Reviewed
• [Journal Article] Bim regulates B-cell receptor-mediated apoptosis in the presence of CD40 signaling in CD40-pre-activated splenic B cells differentiating into plasma cells (2012)
  • Author(s): Gao Y, Kazama H and Yonehara S
  • Journal Title: Int Immunol Volume: 24 Pages: 283-292
  • Peer Reviewed
• [Journal Article] SR-PSOX/CXCL16 plays a critical role in the progression of colonic inflammation (2011)
  • Author(s): Uza N, Nakase H, Yamamoto S, Yoshino T, Takeda Y, Ueno S, Inoue S, Mikami S, Matsuura M, Shimaoka T, Kume N, Minami M, Yonehara S, Ikeuchi H, and Chiba T
  • Journal Title: Gut Volume: 60 Pages: 1494-1505
  • Peer Reviewed
• [Journal Article] Identification of functional regions defining different activity between caspase-3 and caspase-7 within cells (2010)
  • Author(s): Nakatsumi H, and Yonehara S
  • Journal Title: J Biol Chem Volume: 285 Pages: 25418-25425
  • Peer Reviewed
• [Presentation] Allergic blepharitis is developed in Balb/c Fas knockout mice with associated Hyper-production of IgE (2012)
  • Author(s): Yonehara S
  • Organizer: 2nd nternational Symposium on Carcinogenic Spiral "Infection, Immunity and Cancer"
  • Place of Presentation: Kyoto.
  • Year and Date: 2012-01-16
• [Presentation] Double-faced functions of caspase-8 in induction and protection of programmed cell death (2012)
  • Author(s): Yonehara S
  • Organizer: The 10th NTUJapan International Mini-Symposium on Molecular and Cell Biology
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2012-01-08
• [Presentation] Apoptosis and Disorders: Allergic Blepharitis Associated with Hyper-Production of IgE in Balb/c Fas Knockout Mouse. (2011)
  • Author(s): Yonehara S
  • Organizer: Bio-Rheumatology International Congress (BRIC2011) Plenary Session -Immunology-
  • Place of Presentation: Urayasu, Chiba
  • Year and Date: 20110114-15
• [Presentation] 新しいタイプのプログラム細胞死 (2011)
  • Author(s): 米原 伸
  • Organizer: 第84回日本生化学会 シンポジウム:多様な生物における細胞死と細胞死関連分子の多彩な機能
  • Place of Presentation: 京都
  • Year and Date: 2011-09-21
• [Presentation] 新しいタイプのプログラムされた細胞死 (2011)
  • Author(s): 米原 伸
  • Organizer: 第20回CellDeath学会学術集会 シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-07-21
• [Presentation] がん研究入門コース. 多様な細胞死とがんとの関わり/Cell Death and Cancer (2010)
  • Author(s): Yonehara S
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23