2014 Fiscal Year Final Research Report
Structural and functional analyses of heme-copper oxygen reductases to elucidate the energy transduction mechanism
| Project/Area Number |
22370060
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biophysics
|
|---|
| Research Institution | University of Hyogo |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2010-04-01 – 2015-03-31
|
| Keywords | 生物物理 / ナノバイオ / 蛋白質 / 酵素 / 呼吸鎖 / 生体エネルギー変換 / X線結晶構造解析 |
|---|
| Outline of Final Research Achievements |
Heme-copper oxygen reductase in respiratory electron transport chain efficiently converts energy released in the reduction reaction of molecular oxygen (electron transfer) to driving force for the active transport of hydrogen ion (proton pump). 1) binding manner of molecular oxygen, 2) O-O bond cleavage mechanism, 3) reaction intermediate structure, 4) the resting form structure, 5) structural change coupled to electron transfer were found based on the x-ray crystal structures of aa3-type, which belongs to the heme-copper oxygen reductase family. Purification method of cbb3-type was established, and property of the electron transfer efficiency was found by comparing the enzymatic activity of cbb3-type with that of aa3-type.
|
| Free Research Field |
生物物理学
|
