2012 Fiscal Year Final Research Report
Molecular analyses on the replication initiation complex and its novel regulatory factors in E. coli
| Project/Area Number |
22370064
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UEDA Tadashi 九州大学, 大学院・薬学研究院, 教授 (90184928)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Keywords | DnaA / DnaB / oriC / ATP / 細胞周期制御 / 染色体複製開始制御 / タンパク質高次複合体 / 試験管内再構成 |
|---|
| Research Abstract |
The initiation of the chromosomal DNA replication requires formation of higher-order nucleoprotein complexes and their functions must be regulated during the cell cycle. In this study, by analyzing higher-order complexes formed by the replication origin DNA and the replication initiator DnaA protein in E. coli as a model cell, we identified subcomplexes important for replication initiation reactions and revealed novel molecular mechanisms. Moreover, by analyzing major factors regulating DnaA functions, we revealed a novel regulatory mechanism for DnaA in addition to novel interaction modes between DnaA and the factors
|
Research Products
(33 results)
-
[Journal Article] The DnaA N-terminal domain interacts with Hda to facilitate replicase clamp-mediated inactivation of DnaA2013
Author(s)
Su'etsugu, M., Harada, Y., Keyamura, K., Matsunaga, C., Kasho, K., Abe, Y., Ueda, T. and Katayama, T.
-
Journal Title
nviron. Microbiol.
Volume: (印刷中)
DOI
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] DnaA, the replication initiator, is activated for the timely initiation of the chromosomal replication by a specific DNA element, DARS2011
Author(s)
Fujimitsu, K., Kasho, K., Ozaki, S., Keyamura, K. and Katayama, T.
Organizer
International Symposium on the Physicochemical Field for Genetic Activities
Place of Presentation
Awaji
Year and Date
20110124-26
-
-
[Presentation] DARS, DnaA-reactivating sequence promotes exchange of DnaA-bound ADP to ATP.2010
Author(s)
Fujimitsu, K., Kasho, K., Ozaki, S., Senriuchi, T. and Katayama, T.
Organizer
EMBO Conference Series; Replication, Repair & Segregation of Chromosomes
Place of Presentation
Germany
Year and Date
20100613-17
-
-
-
-
-
