Regulation of cell functions by deubiquitinating enzymes

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370068
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: KOMADA Masayuki 東京工業大学, 大学院・生命理工学研究科, 准教授 (10225568)
• Project Period (FY): 2010 – 2012
• Keywords: タンパク質分解

Research Abstract

1) Deubiquitinating enzyme USP18 was implicated in the protection of cells from the toxicity of non-native defective proteins by sequestering them to the aggresome in human cells.2) Small molecules that bind to the nucleolar localization signal of deubiquitinating enzyme USP36 were identified and were shown to inhibit the nucleolar localization as well as the function of USP36 in human cells. 3) The ubiquitin-interacting motifs (UIMs) in USP37 were shown to play a role in increasing the isopeptidase activity of USP37 toward ubiquitin chains by binding to the ubiquitin chains and stably positioning them in close proximity to the catalytic core of USP37.

Research Products

• [Journal Article] ERK-dependent down-of UIM-bearing proteins regulates EGF receptor endocytosis from the plasma membrane (2013)
  • Author(s): Li, X, Mr. Bian, Y., Takizawa, Y., Hashimoto, T., Ikoma, T., Dr. Tanaka, J., Kitamura, N., Inagaki, Y., Komada, M., and Tanaka, T
  • Journal Title: Mol. Biol.Cell Volume: 23 Pages: 1343-1353
  • Peer Reviewed
• [Journal Article] In vivo assembly of the axon initial segment in motor neurons (2013)
  • Author(s): Le Bras, B., Freal, A., Czarnecki, A., Legendre, P., Bullier, E., Komada, M., Brophy, P.J., Davenne, M., and Couraud, F
  • Journal Title: Brain Struct. Funct
  • DOI: doi:10.1007/s00429-013-0578-7.
  • Peer Reviewed
• [Journal Article] The ubiquitin code and its decoding machinery in the endocytic pathway (2013)
  • Author(s): Tanno, H. and Komada, M.
  • Journal Title: J. Biochem Volume: 153 Pages: 497-504
  • Peer Reviewed
• [Journal Article] Involvement of Src in the membrane skeletal complex, MPP6-4.1G, in Schmidt-Lanterman incisures of mouse myelinated nerve fibers in PNS (2013)
  • Author(s): Terada, N., Saitoh, Y., Ohno, N., Komada, M., Yamauchi, J., and Ohno, S.
  • Journal Title: Histochem. Cell Biol
  • DOI: doi:10.1007/ s00418-012-1073-6.
  • Peer Reviewed
• [Journal Article] ユビキチン化によるFrizzledのリソソーム 分解を介した Wnt シグナル強度の制御 (2013)
  • Author(s): Daocharad Burana、後藤聡、駒田雅之
  • Journal Title: 細胞工学 特集「Wnt協奏曲」 Volume: 32 Pages: 396-400
• [Journal Article] Balanced ubiquitination determines cellular responsiveness to extracellular stimuli (2012)
  • Author(s): Mukai, A., Yamamoto-Hino, M., Komada, M., Okano, H., and Goto, S.
  • Journal Title: Cell. Mol. Life Sci. Volume: 69 Pages: 4007-4016
  • Peer Reviewed
• [Journal Article] The Ankrd 13 family of UIM-bearing proteins regulates EGF receptor endocytosis from the plasma membrane (2012)
  • Author(s): Tanno, H., Yamaguchi, T., Goto, E., Ishido, S., and Komada, M.
  • Journal Title: Mol. Biol. Cell Volume: 23 Pages: 1343-1353
  • Peer Reviewed
• [Journal Article] Essential function of protein 4.1G in targeting of MPP6 into Schmidt-Lanterman incisures in myelinated nerves (2012)
  • Author(s): Terada, N., Saitoh, Y., Ohno, N., Komada, M., Saitoh, S., Peles, E., and Ohno, S.
  • Journal Title: Mol. Cell. Biol Volume: 32 Pages: 199-205
  • Peer Reviewed
• [Journal Article] Nrk, an X-linked protein kinase in the germinal center kinase family, is required for placental development and fetoplacental induction of labor (2011)
  • Author(s): Denda, K., Nakao-Wakabayashi, K., Okamoto, N., Kitamura, N., Ryu, J.-Y., Tagawa, Y., Ichisaka, T., Yamanaka, S., and Komada, M
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 28802-28810
  • Peer Reviewed
• [Journal Article] Leucine-rich repeat kinase LRRK1 regulates endosomal trafficking of the EGF regulation of Skp2 reduces Myc activity with receptor (2011)
  • Author(s): Hanafusa, H., Ishikawa, K., Kedashiro, S., Saigo, T., Iemura, S., Natsume, T., Komada, M., Shibuya, H., Nara, A., and Matsumoto, K
  • Journal Title: Nature Commun. 2 Volume: 158
  • Peer Reviewed
• [Journal Article] ユビキチン化による核小体の構造と機能の制御 (2011)
  • Author(s): 駒田雅之
  • Journal Title: 生体の科学特集「細胞核-構造と機能」 Volume: 62 Pages: 422-423
• [Journal Article] Balanced ubiquitylation and deubiquitylation of Frizzled regulate cellular responsiveness to Wg/Wnt (2010)
  • Author(s): Mukai, A., Yamamoto-Hino, M., Awano, W., Watanabe, W., Komada, M., and Goto, S.
  • Journal Title: EMBO J. Volume: 29 Pages: 2114-2125
  • Peer Reviewed
• [Journal Article] Dominant-negative mutations in α-II spectrin cause West syndrome with severe cerebral hypomyelination, spastic quadriplegia, and developmental delay (2010)
  • Author(s): Saitsu, H., Tohyama, J., Kumada, T., Egawa, K., Hamada, K., Okada, I., Mizuguchi, T., Osaka, H., Miyata, R., Furukawa, T., Haginoya, K., Hoshino, H., Goto, T., Hachiya, Y., Yamagata, T., Saitoh, S., Nagai, T., Nishiyama, K., Nishimura, A., Miyake, N., Komada, M., Hayashi, K., Hirai, S., Ogata, K., Kato, M., Fukuda, A., and Matsumoto, N.
  • Journal Title: Am. J. Hum. Genet Volume: 86 Pages: 881-891
  • Peer Reviewed
• [Journal Article] Activated microglia mediate axo-glial disruption that contributes to axonal injury in multiple sclerosis (2010)
  • Author(s): Howell, O.W., Rundle, J.L., Garg, A., Komada, M., Brophy, P.J., and Reynolds, R.
  • Journal Title: J. Neuropathol. Exp. Neurol. Volume: 69 Pages: 1017-1033
  • Peer Reviewed
• [Journal Article] Involvement of a membrane skeletal protein, 4.1G, for Sertoli/germ cell interaction. (2010)
  • Author(s): Terada, N., Ohno, N., Saitoh, S., Saitoh, Y., Komada, M., Kubota, H., and Ohno, S.
  • Journal Title: Reproduction Volume: 139 Pages: 883-892
  • Peer Reviewed
• [Journal Article] 受容体のユビキチン化と脱ユビキチン化を介した細胞のWnt応答性の制御 (2010)
  • Author(s): 駒田雅之、後藤聡
  • Journal Title: 細胞工学特集「拡大するユビキチンバイオロジー」 Volume: 29 Pages: 1237-1243
• [Journal Article] 脱ユビキチン化酵素群の細胞機能 (2010)
  • Author(s): 駒田雅之、遠藤彬則
  • Journal Title: 生化学 Volume: 82 Pages: 378-387
  • Peer Reviewed
• [Journal Article] 軸索に特異的なスペクトリン細胞膜骨格の機能 (2010)
  • Author(s): 駒田雅之
  • Journal Title: 生体の科学特集「シナプスをめぐるシグナリング」 Volume: 61 Pages: 498-499
• [Presentation] タイトル未定 (2013)
  • Author(s): 駒田雅之
  • Organizer: 日本分子生物学会大会・ワークショップ-ER・Post-ERにおける膜プロテオスタシスネットワーク研究の新展開
  • Place of Presentation: 神戸国際会議場、神戸
  • Year and Date: 20131236
• [Presentation] Rolesof the Ankrd 13 familyofubiquitin-bindingproteinsintheendocyticpathway (2013)
  • Author(s): 駒田雅之
  • Organizer: 日本生化学会大会・インターナショナルセッション・リソソーム生物学のフロンティア
  • Place of Presentation: パシフィコ横浜、横浜
  • Year and Date: 2013-09-11
• [Presentation] ユビキチン化と脱ユビキチン化によるストレス顆粒の制御 (2013)
  • Author(s): 駒田雅之
  • Organizer: 日本細胞生物学会大会・シンポジウム-タンパク質分解システムによる細胞制御
  • Place of Presentation: ウイング愛知、名古屋
  • Year and Date: 2013-06-21
• [Presentation] 脱ユビキチン化酵素による細胞のストレス応答の制御 (2012)
  • Author(s): 駒田雅之
  • Organizer: 日本生化学会大会・シンポジウム-ベールを脱いだユビキチン系の新機能
  • Place of Presentation: 福岡国際会議場、福岡
  • Year and Date: 2012-12-16
• [Presentation] 新規ユビキチン結合因子Ankrd13によるエンドサイトーシス制御 (2011)
  • Author(s): 丹野秀崇、山口鉄平、後藤栄治、石戸聡、駒田雅之
  • Organizer: 日本生化学会大会・シンポジウム-拡大するユビキチンバイオロジー
  • Place of Presentation: 国立京都国際会館、京都
  • Year and Date: 2011-09-23
• [Book] 生化学事典 (2011)
  • Author(s): 駒田雅之
  • Publisher: 「エンドサイトーシス」「FYVEドメイン」