2013 Fiscal Year Final Research Report
Evolutionary mechanisms of the gastrula organizer and blastopore formation by focusing on the gene regulatory network
| Project/Area Number |
22370075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAIRA Masanori 東京大学, 理学(系)研究科(研究院), 准教授 (60150083)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Keywords | クロマチン免疫沈降 / 原腸胚オーガナイザー / ネッタイツメガエル / アフリカツメガエル |
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| Research Abstract |
In early vertebrate development, the basic body plan is established by the gastrula organizer. To examine the role of several transcription factors that play a central role in this important tissue as well as its evolutionary scenario, we carried out chromatin-immunoprecipitation sequencing (ChIP-seq) analysis using Xenopus gastrula embryos. The data suggest that transcription factors Otx2, Lim1/Lhx1, Gsc, and Sia combinatorially bind to gene regulatory regions, and positively and negatively regulate several hundreds of genes. In addition, comparative functional analysis of regulatory regions between Xenopus and an ancient type of vertebrates, amphioxus, suggest a scenario, in which evolution in activities of regulatory regions of some organizer genes contributed for the vertebrate ancestor to have acquired the head structure.
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[Journal Article] Occupancy of Tissue-Specific cis-Regulatory Modules by Otx2 and TLE/Groucho for Embryonic Head Specification2014
Author(s)
Yasuoka Y., Suzuki Y., Takahashi S., Sudou N., Haramoto Y., Tando Y., Kubokawa K., Cho K. W., Asashima M., Sugano S., and Taira M.
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Journal Title
Nat. Commun
Volume: (in press)
Peer Reviewed
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[Journal Article] The medaka enhancer mutant for zic1/zic4 provides molecular insights into teleost caudal fin evolution2012
Author(s)
Moriyama Y., Kawanishi T., Nakamura R., Tsukahara T., Sumiyama K., Suster M., Kawakami K., Toyoda A., Fujiyama A., Yasuoka Y., Nagao Y., Sawatari E., Shimikzu A., Wakamatsu Y., Hibi M., Taira M., Okabe M., Naruse K., Hashimoto H., Shimada A., Takeda H.
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Journal Title
Curr. Biol.
Volume: 22
Pages: 601-607
DOI
Peer Reviewed
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[Presentation] Massively parallel regulation of head and non-head genes by Otx2, Lim1 and Gsc is based on evolution of the head organizer in the chordate2012
Author(s)
Yasuoka Y., Suzuki Y., Takahashi S., Sudou N., Haramoto Y., Tando Y., Kubokawa K., Cho K. W., Asashima M., Sugano S., and Taira M.
Organizer
日本発生生物学会第45回大会
Place of Presentation
神戸
Year and Date
20120528-31
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[Presentation] Direct contributions of Otx2 as a positional tag to global gene regulation in the head organizer in Xenopus embryos2011
Author(s)
Yasuoka Y., Suzuki Y., Takahashi S., Sudou N., Haramoto Y., Asashima M., Sugano S., and Taira M.
Organizer
International Symposium"Genetic Regulation of Development"Commemorative of 27th International Prize for Biology, 2011, Celebrating Dr. Eric Davidson
Place of Presentation
京都ガーデンパレス,京都
Year and Date
20111130-1201
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