2013 Fiscal Year Final Research Report
Molecular cytogenetic studies on the process of genomic and chromosomal reorganization in amphibians and reptiles
| Project/Area Number |
22370081
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Nagoya University |
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Principal Investigator |
MATSUDA Yoichi 名古屋大学, 生命農学研究科, 教授 (70165835)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Chizuko 北海道大学, 大学院理学研究院, 助手 (50106580)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Keywords | 両生類 / 爬虫類 / FISH / ゲノム重複 / 核型進化 / 染色体地図 / マイクロ染色体 / ゲノムの区画化 |
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| Research Abstract |
To delineate the process of genomic and karyotypic evolution of tetrapods, we constructed comparative cytogenetic maps of Pelodiscus sinensis, Crocodylus siamensis, Elaphe quadrivirgata, Varanus salvator, Xenopus tropicalis, and Xenopus laevis. Our results provide the possibility that the ancestral karyotype of tetrapods had at least 10 large linkage groups of macrochromosomes and many microchromosomal pairs, which have been conserved in the chicken. We identified all nine homoeologous chromosome groups of X laevis, which were homologous within the same groups except for two inversions, and the genetic linkages were highly conserved between X. tropicalis and X. laevis except for one fusion and one inversion. In E. quadrivirgata, microchromosomal genes showed significantly higher GC content than macrochromosomal genes as in the chicken, suggesting that the chromosome size-dependent GC heterogeneity had already occurred before the lepidosaur-archosaur split, 275 million years ago.
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[Journal Article] Highly species-specific centromeric repetitive DNA sequences in lizards : molecular cytogenetic characterization of a novel family of satellite DNA sequences isolated from the water monitor lizard (Varanus salvator macromaculatus, Platynota)2013
Author(s)
Chaiprasertsri N, Uno Y, Peyachoknagul S, Prakhongcheep O, Baicharoen S, Charernsuk S, Nishida C, Matsuda Y, Koga A, Srikulnath K
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Journal Title
J. Hered.
Volume: 104
Pages: 798-806
DOI
Peer Reviewed
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[Journal Article] Inference of the protokaryotypes of amniotes and tetrapods and the evolutionary processes of microchromosomes from comparative gene mapping2012
Author(s)
Uno Y, Nishida C, Tarui H, Ishishita S, Takagi C, Nishimura O, Ishijima J, Ota H, Kosaka A, Matsubara K, Murakami Y, Kuratani S, Ueno N, Agata K, Matsuda Y
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Journal Title
PLoS One
Volume: 7
Pages: e53027
DOI
Peer Reviewed
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