2013 Fiscal Year Final Research Report
Production of apple sport by epigenetic variation system
Project/Area Number |
22380003
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Breeding science
|
Research Institution | Hirosaki University |
Principal Investigator |
HARADA Takeo 弘前大学, 農学生命科学部, 教授 (10228645)
|
Co-Investigator(Kenkyū-buntansha) |
SENDA Mineo (30261457)
|
Project Period (FY) |
2010-04-01 – 2014-03-31
|
Keywords | ジーンサイレンシング / siRNA / リンゴ / 日持ち性 |
Research Abstract |
Using the scion in which the construct producing an artificial siRNA sequence was integrated, its siRNA molecule transported through phloem functions to induce the silencing (TGS: transcriptional gene silencing) of the gene possessing complementary sequence with the siRNA. In case of TGS, as the invoked silencing maintains through DNA replication, the TGS plant can be obtained by the regeneration from the TGS tissue. This study was carried out the each step experiment in order to apply the above system to a vegetative crop, apple (Malus domestica).
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