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2013 Fiscal Year Final Research Report

Production of apple sport by epigenetic variation system

Research Project

  • PDF
Project/Area Number 22380003
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionHirosaki University

Principal Investigator

HARADA Takeo  弘前大学, 農学生命科学部, 教授 (10228645)

Co-Investigator(Kenkyū-buntansha) SENDA Mineo   (30261457)
Project Period (FY) 2010-04-01 – 2014-03-31
Keywordsジーンサイレンシング / siRNA / リンゴ / 日持ち性
Research Abstract

Using the scion in which the construct producing an artificial siRNA sequence was integrated, its siRNA molecule transported through phloem functions to induce the silencing (TGS: transcriptional gene silencing) of the gene possessing complementary sequence with the siRNA. In case of TGS, as the invoked silencing maintains through DNA replication, the TGS plant can be obtained by the regeneration from the TGS tissue. This study was carried out the each step experiment in order to apply the above system to a vegetative crop, apple (Malus domestica).

  • Research Products

    (29 results)

All 2014 2013 2012 2011 2010 2008 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (12 results) Book (5 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] GIBBERELLIC ACID INSENSITIVE transcript transported from stock is translated and attenuates GA response in the scion2013

    • Author(s)
      Xu H, Iwashiro R, Li T, Harada T
    • Journal Title

      BMC Plant Biology

      Volume: 13 Pages: 165

    • DOI

      10.1186/10.1186/1471-2229-13-165

    • Peer Reviewed
  • [Journal Article] Scion on a stock producing siRNAs of potato spindle tuber viroid (PSTVd) attenuates accumulation of the viroid2013

    • Author(s)
      Kasai A, Sano T, Harada T
    • Journal Title

      PLOS ONE

      Volume: 8(2) Pages: e57736

    • DOI

      10.1371/journal.pone.0057736

    • Peer Reviewed
  • [Journal Article] Transport of mRNA molecules coding NAC domain protein in grafted pear and transgenic tabacco2012

    • Author(s)
      Zhang WN, Duan XW, Ma C, Harada T, Li TZ
    • Journal Title

      Biologia Plantarum

      Volume: 57 Pages: 224-230

    • DOI

      10.1007/s11105-011-7

    • Peer Reviewed
  • [Journal Article] Distribution of MdACS3 null alleles in apple (Malus×domestica Borkh.) and its relevance to the fruit ripening characters2012

    • Author(s)
      Bai S, Wang A, Igarashi M, Kon T, Fukasawa-Akada T, Li T, Harada T, Hatsuyama Y
    • Journal Title

      Breeding Science

      Volume: 62 Pages: 46-52

    • DOI

      10.1270/jsbbs.62.46

    • Peer Reviewed
  • [Journal Article] Gibberellic acid-insensitive mRNA transport in Pyrus2011

    • Author(s)
      Zhang WN, Gong L, Ma C, Xu HY, Hu JF, Harada T, Li TZ.
    • Journal Title

      Plant Molecular Biology Reporter

    • DOI

      10.1007/s11105-011-7

    • Peer Reviewed
  • [Journal Article] The regulatory role of 1-aminocyclopropan-1-carboxylate synthase genes in apple fruit shelf life2011

    • Author(s)
      Wang A, Li T, Harada T
    • Journal Title

      Europ J. Horticult. Sci

      Volume: 76 Pages: S.77-83

    • Peer Reviewed
  • [Journal Article] Mobile signal transported over a long distance induces systemic transcriptional gene silencing in a grafted partner2011

    • Author(s)
      Bai S, Kasai A, Yamada K, Li T, Harada T
    • Journal Title

      J. Exp. Bot

      Volume: 62 Pages: 4561-4570

    • DOI

      10.1093/jxb/err163

    • Peer Reviewed
  • [Journal Article] The Arabidopsis CORI3 promoter contains two cis-acting regulatory regions required for transcriptional activity in companion cells2011

    • Author(s)
      Tsuwamoto R, Harada T
    • Journal Title

      Plant Cell Reports

      Volume: 30 Pages: 1723-1733

    • DOI

      10.1007/s00299-011-1080-4

    • Peer Reviewed
  • [Journal Article] Graft-transmitted siRNA signal from root induces visual manifestation of endogenous post transcriptional gene silencing in the scion2011

    • Author(s)
      Kasai A, Bai S, Li T, Harada T
    • Journal Title

      PLoS ONE

      Volume: 6 Pages: e16895

    • DOI

      10.1371/journal.pone.0016895

    • Peer Reviewed
  • [Presentation] 接ぎ木と siRNA篩管輸送による品種改良2014

    • Author(s)
      原田竹雄・北條初音・葛西厚史
    • Organizer
      日本農芸化学会シンポジウムアプライド RNAサイエンス-産業利用を目指した微生物・植物・動物の RNA研究-
    • Place of Presentation
      明治大学
    • Year and Date
      2014-03-30
  • [Presentation] ジャガイモの新奇エピゲノム編集法2014

    • Author(s)
      葛西厚史・北條初音・原田竹雄
    • Organizer
      日本育種学会
    • Place of Presentation
      東北大学
    • Year and Date
      2014-03-22
  • [Presentation] Epimutant induction of endogenous gene by grafting2013

    • Author(s)
      Harada T
    • Organizer
      29th IPSR International Symposium and Symposium on Plant Stress Sciences
    • Place of Presentation
      岡山大学資源植物科学研究所
    • Year and Date
      20130307-08
  • [Presentation] Epimutant induction by long-distance transport siRNA2013

    • Author(s)
      Kasai A, Hojo H, Harada T
    • Organizer
      8th Joint Korea-Japan Plant Biotech Workshop -New Biotechnology-based Plant Breeding Techniques-
    • Place of Presentation
      Hokkaido University
    • Year and Date
      2013-09-09
  • [Presentation] Transcriptional gene silencing of endogenous gene Virp1 in tomato2013

    • Author(s)
      Hojo H, Kasai A, Harada T
    • Organizer
      Plant Biology 2013
    • Place of Presentation
      USA (Boston)
    • Year and Date
      2013-07-23
  • [Presentation] An effective TGS induction in grafting partner by phloem transport of siRNA and mRNA2013

    • Author(s)
      Kasai A, Iwashiro R, Harada T
    • Organizer
      Plant Biology 2013
    • Place of Presentation
      USA (Boston)
    • Year and Date
      2013-07-23
  • [Presentation] トマト SlVirp1エピ変異体の獲得とその特性法2013

    • Author(s)
      葛西厚史・北條初音・佐野輝男・原田竹雄
    • Organizer
      日本育種学会
    • Place of Presentation
      東京農業大学
    • Year and Date
      2013-03-24
  • [Presentation] 篩管輸送 RNAと根系機能活用による新規形質転換体作出法2012

    • Author(s)
      原田竹雄
    • Organizer
      園芸学会平成24年度秋季大会シンポジウム
    • Place of Presentation
      福井県立大学
    • Year and Date
      2012-09-22
  • [Presentation] エピ変異体獲得におけるRNA篩管輸送モチーフの活用2012

    • Author(s)
      原田竹雄・岩城麗香・岩本啓志・葛西厚史
    • Organizer
      日本育種学会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-09-15
  • [Presentation] 接ぎ木を利用した内生遺伝子のエピ変異体獲得について2012

    • Author(s)
      北條初音・葛西厚史・原田竹雄
    • Organizer
      日本育種学会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-09-15
  • [Presentation] 植物の接ぎ木を利用した接ぎ木相手の形質転換2012

    • Author(s)
      原田竹雄
    • Organizer
      日本学術会議公開シンポジウム「新しい遺伝子組換え技術の開発と植物研究・植物育種への利用」
    • Year and Date
      2012-05-14
  • [Presentation] Generation of Epimutants by Grafting

    • Author(s)
      Kasai A, Hojo H, Kawamata T, Harada T
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      00001211-14
  • [Book] 接ぎ木によって接ぎ木相手を改良する B&Iバイオサイエンスとインダストリー 722014

    • Author(s)
      葛西厚史・原田竹雄
    • Total Pages
      237-239
  • [Book] 植物におけるエピゲノム編集:その原理と接ぎ木による誘導生物の科学遺伝 32014

    • Author(s)
      葛西厚史・原田竹雄
    • Total Pages
      140-144
  • [Book] エピ変異誘導による品種改良;遺伝子の環境記憶システムの活用。ゲノム手法・情報を利用した果樹研究の展開 No.9果実日本 692014

    • Author(s)
      原田竹雄・葛西厚史
    • Total Pages
      2
  • [Book] 植物の接ぎ木を利用した接ぎ木相手の形質転換:植物の成長調節2013

    • Author(s)
      葛西厚史・原田竹雄
    • Total Pages
      126-134
    • Publisher
      Regulation of Plant Growth & Development 48
  • [Book] 植物育種技術としてのエピ変異体作出法(シリーズ: NBT⑤バイオサイエンスとインダストリー 71)2013

    • Author(s)
      葛西厚史・原田竹雄
    • Total Pages
      462-465
  • [Remarks]

    • URL

      http://nature.cc.hirosaki-u.ac.jp/lab/1/tharada/index.html

  • [Patent(Industrial Property Rights)] 台木と穂木の接ぎ木を介して植物の形質転換を行う方法2010

    • Inventor(s)
      原田竹雄、葛西厚史、白松齢、山田かおり
    • Industrial Property Rights Holder
      弘前大学
    • Industrial Property Rights Type
      ライフサイエンス特開2013-201911
    • Patent Publication Number
      特開2013-201911
    • Acquisition Date
      2010-12-20
    • Overseas
  • [Patent(Industrial Property Rights)] 植物の篩管を通して遺伝子の転写物を輸送する方法2008

    • Inventor(s)
      原田竹雄、工藤久幸
    • Industrial Property Rights Holder
      弘前大学
    • Industrial Property Rights Type
      ライフサイエンス
    • Industrial Property Number
      特許第5360702号
    • Filing Date
      2008-09-26
    • Overseas

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Published: 2015-06-25  

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