2012 Fiscal Year Final Research Report
Genetic engineering of chloroplast genome using a large-chloroplast mutant in rice
| Project/Area Number |
22380007
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Okayama University |
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Principal Investigator |
SAKAMOTO Wataru 岡山大学, 資源植物科学研究所, 教授 (20222002)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAZONO Mikio 名古屋大学, 大学院・生命農学研究科, 教授 (70282697)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 葉緑体ゲノム / 遺伝育種学 / 遺伝子導入 / イネ / 葉緑体分裂装置 |
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| Research Abstract |
Genetic engineering of chloroplast genome in higher plants has been attempted since 1980’s, and has been successful in tobacco and several species. Chloroplast transformation has advantages over nucleargenome, because i) maternal inheritance of the genome eliminates dispersal of a transgene through pollen, ii) no gene silencing allows transgene to be expressed at highlevel, and iii) homologous recombination targets a transgene into a desired genomic region. Despite such advantages, efficiency of chloroplast transformation is extremely low in rice and other crops. We reasoned that one obstacle may be a small size of chloroplasts in these species. In this study, we propose to utilize a mutant that has enlarged chloroplasts. Isolation and characterization of a rice mutant showing large chloroplasts, identification of the gene responsible for this mutation, and an attempt to use this line for rice chloroplast transformation, have been conducted.
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