2012 Fiscal Year Final Research Report
Digital gene expression QTL analysisas an efficient gene mapping tool
| Project/Area Number |
22380009
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Shinshu University |
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Principal Investigator |
MATSUMURA Hideo 信州大学, ヒト環境科学研究支援センター, 准教授 (40390885)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ゲノム育種 / 遺伝子発現解析 / DNA マーカー |
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| Research Abstract |
We aimed at developing a novel QTL (quantitative trait locus) mapping procedure based on genome-wide gene expression analysisdata in rice. In the present study, quantitative digital gene expression analysis method was developed, allowed to analyzing expression of thousands of gene in multiple samples, like segregated individuals. Additionally, a novel tool for exploring DNA polymorphisms was also established and it enabled to genotyping many loci at a time. These techniques were basis of digital expression QTL analysis.
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