Study on the mechanism of dual resistance to virus and oomycete using their chimeric resistance genes

2014 Fiscal Year Final Research Report

• Project/Area Number: 22380028
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Hideki 東北大学, (連合)農学研究科(研究院), 教授 (20197164)
• Co-Investigator(Kenkyū-buntansha): ANDO Sugihiro 東北大学, 大学院農学研究科, 准教授 (10442831)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: ウイルス抵抗性 / キュウリモザイクウイルス / 病害抵抗性遺伝子 / NB-LRRタンパク質

Outline of Final Research Achievements

RCY1 confers the hypersensitive resistance (HR) response to Cucumber mosaic virus [CMV(Y)] in A. thaliana, whereas its allelic RPP8 does the resistance to H. arabidopsidis. When RCY1, RPP8 and a series of chimeric constructs between RCY1 and RPP8 were transiently expressed in N. benthamiana, respectively, HR cell death was induced in the leaf tissue expressing chimeric construct carrying CC-NB of RPP8 and LRR of RCY1, suggesting that LRR domain of RCY1 determines the recognition of CMV(Y). Furthermore, to study on the regulatory mechanism of RCY1/RPP8 gene expression, we analyzed the contribution of each structural element of the gene. As the result, we can find that the presence of intron in RCY1/RPP8 genes is indispensable for higher level of RCY1/RPP8 protein accumulation. Those findings regarding pathogen recognition specificity and the role of intron for the resistance to pathogens will give new insight to understand the plant immune system against pathogen infection.

Free Research Field

植物病理学