2012 Fiscal Year Final Research Report
Construction of disease-resistant plants by controlling the suppressor function of avirulence effector
| Project/Area Number |
22380029
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | Shizuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Hisae 静岡大学, 農学部, 准教授 (00432196)
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| Project Period (FY) |
2010 – 2012
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| Keywords | カンキツかいよう病菌 / PthAエフェクター / Pectin methyl esterase 前駆体 / RIN4 / UPAボックス / 非病原力遺伝子 / AvrBs3ファミリー |
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| Research Abstract |
We had found that avirulence factors possess the suppressor function in addition to the common eliciting function of cultivar-specific resistance responses. PthA, a canker-forming effector with homology to the avirulence effector (AvrBs3) group commonly found in xanthomonads, was found to possess the suppressor function too. Here we found that PthA can bind specifically to the precursor for pectin methyl esterase (PME) of Citrus led to the new strategy to create the canker-resistant Citrus by the introduction of the PME precursors of other plant sources together with the introduction of mutation into the citrus PME precursor or gene silencing of PME precursor gene of Citrus by RNA interference. We suggest this modification of the suppressor activities of avirulence effectors by modification of their binding protein in the host plants as a new strategy for the construction of disease-resistant plants.
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