An alternative strategy for adaptation to acidic soil in plants: molecular basis of symbiotic network with microorganisms

2013 Fiscal Year Final Research Report

• Project/Area Number: 22380042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: Hokkaido University
• Principal Investigator: EZAWA Tatsuhiro 北海道大学, (連合)農学研究科(研究院), 准教授 (40273213)
• Co-Investigator(Kenkyū-buntansha): AONO Toshihiro 東京大学, 生物生産工学研究センター, 助教 (10372418)
• Co-Investigator(Renkei-kenkyūsha): MASTA Chikara 北海道大学, 大学院農学研究院, 教授 (60281854) ; KAWAHARA Ai 北海道大学, 大学院農学院, 博士課程 ; KIKUCHI Yusuke 北海道大学, 大学院農学院, 博士課程 ; YOKOYAMA Kaede 北海道大学, 大学院農学院, 博士課程
• Project Period (FY): 2010-04-01 – 2013-03-31
• Keywords: アーバスキュラー菌根菌 / 共生 / 菌類ウィルス / 植物耐酸性 / リン酸 / dsRNA

Research Abstract

Soil acidity inhibits plant root growth and thus constrains plant productivity. Acid-tolerant arbuscular mycorrhizal (AM) fungi allow plant survival in acidic soil. The present study investigated the mechanisms underlying the enhancement of plant acid-tolerance by AM fungi. Acid-tolerant AM fungi provide an alternative pathway of phosphate uptake for the damaged roots. Genes involved in phosphate uptake and their expression in response to increased phosphate availability were demonstrated in an acid-tolerant fungus by transcriptome analysis. Four double-stranded RNA viruses were identified in an acid-tolerant fungus, of which a role in acid-tolerance of the host fungus will be investigated in the future.

Research Products

• [Journal Article] A unique mitovirus from Glomeromycota, the phylum of arbuscular mycorrhizal fungi (2014)
  • Author(s): Kitahara R., Ikeda Y., Shimura H., Masuta C., and Ezawa T.
  • Journal Title: Archives of Virology Volume: (in press)
  • DOI: 10.1007/s00705-014-1999-1.
  • Peer Reviewed
• [Journal Article] Characterization of arbuscular mycorrhizal fungal communities with respect to zonal vegetation in a coastal dune ecosystem (2013)
  • Author(s): Kawahara A., and Ezawa T.
  • Journal Title: Oecologia Volume: 173 Pages: 533-543
  • DOI: 10.1007/s00442-013-2622-y
  • Peer Reviewed
• [Journal Article] Ninety-year-, but not single, application of phosphorus fertilizer has a major impact on arbuscular mycorrhizal fungal communities (2013)
  • Author(s): Cheng Y., Ishimoto K., Kuriyama Y., Osaki M., and Ezawa T.
  • Journal Title: Plant and Soil Volume: 365 Pages: 397-407
  • DOI: 10.1007/s11104-012-1398-x
  • Peer Reviewed
• [Journal Article] A novel virus-like double-stranded RNA in an obligate biotroph arbuscular mycorrhizal fungus : a hidden player in mycorrhizal symbiosis (2012)
  • Author(s): Ikeda Y., Shimura H., Kitahara R., Masuta C., and Ezawa T.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 25 Pages: 1005-1012
  • DOI: 10.1094/MPMI-11-11-0288
  • Peer Reviewed
• [Journal Article] 強酸性土壌に棲息するアーバスキュラー菌根菌:パイオニア植生を支える「化石的」絶対共生菌 (2012)
  • Author(s): 江沢辰広,河原愛
  • Journal Title: 生物科学 Volume: 64 Pages: 238-246
  • URL: http://hdl.handle.net/2115/48964
  • Peer Reviewed
• [Journal Article] Polyphosphate has a central role in the rapid and massive accumulation of phosphorus in extraradical mycelium of an arbuscular mycorrhizal fungus (2010)
  • Author(s): Hijikata N., Murase M., Tani C., Ohtomo R., Osaki M., and Ezawa T.
  • Journal Title: New Phytologist Volume: 186 Pages: 285-289
  • DOI: 10.1111/j.1469-8137.2009.03168.x
  • Peer Reviewed
• [Journal Article] 耐酸性菌根菌を活用した強酸性法面緑化適用の可能性 (2010)
  • Author(s): 田中淳,堀江直樹,江澤辰広,伴資英
  • Journal Title: 日緑工誌 Volume: 36 Pages: 119-122
  • DOI: 10.7211/jjsrt.36.119
  • Peer Reviewed
• [Presentation] Responses of arbuscular mycorrhizal fungal communities to soil acidity : acid-tolerant fungi are the generalists that occur in a wide range of soil pH (2013)
  • Author(s): Kawahara A., An G.H., Miyakawa S., Sonoda J., and Ezawa T.
  • Organizer: 7th International Conference on Mycorrhizas
  • Place of Presentation: New Delhi, India
  • Year and Date: 20130106-11
• [Presentation] Acid-tolerant arbuscular mycorrhizal fungi provide an alternative pathway of plant nutrient uptake in acidic soil (2013)
  • Author(s): Kawahara A., and Ezawa T.
  • Organizer: 7th International Conference on Mycorrhizas
  • Place of Presentation: New Delhi, India
  • Year and Date: 20130106-11
• [Presentation] Viruses in arbuscular mycorrhizal fungi : a new player in the symbiosis (2013)
  • Author(s): Kitahara R., Ikeda Y., Shimura H., Masuta C., and Ezawa T.
  • Organizer: 7th International Conference on Mycorrhizas
  • Place of Presentation: New Delhi, India
  • Year and Date: 20130106-11
• [Presentation] アーバスキュラー菌根菌におけるBaas Becking仮説の検証−多重環境フィルタを生き抜く「最強ジェネラリスト」存在の可能性− (2013)
  • Author(s): 江沢辰広,河原愛,荒川竜太
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
  • Year and Date: 2013-09-12
  • Invited
• [Presentation] アルミニウム耐性植物ススキのリン酸吸収を担う直接および菌根経路−強酸性土壌におけるリン酸輸送体遺伝子群の発現と菌根経路の重要性− (2013)
  • Author(s): 河原愛,佐藤理子,江沢辰広
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 名古屋
  • Year and Date: 2013-09-12
• [Presentation] 耐酸性アーバスキュラー菌根菌は広範な環境に分布するジェネラリストである—フィールド調査およびモデル実験の統合— (2012)
  • Author(s): 河原愛,安起弘,宮川祥江,園田順,江沢辰広
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取
  • Year and Date: 2012-09-04
• [Presentation] 多様なウィルスを保持するアーバスキュラー菌根菌Glomus sp. RF1株−2.9 kb-RNAウィルスのゲノム構造と系統進化学的位置− (2012)
  • Author(s): 北原涼子,池田庸二,志村華子,増田税,江沢辰広
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: 鳥取
  • Year and Date: 2012-09-04
• [Presentation] Responses of arbuscular mycorrhizal fungal communities to soil acidity : a synthesis of field studies and a model experiment (2011)
  • Author(s): Kawahara A., An G.H., Miyakawa S., Sonoda J., Osaki M., and Ezawa T.
  • Organizer: Rhizosphere
  • Place of Presentation: Perth, Australia
  • Year and Date: 20110925-30
• [Presentation] A novel mycovirus in an obligate biotroph arbuscular mycorrhizal fungus : a hidden player in mycorrhizal symbiosis (2011)
  • Author(s): Ikeda Y., Shimura H., Masuta C., and Ezawa T.
  • Organizer: 2nd International Mycovirus Symposium
  • Place of Presentation: Otaru, Japan
  • Year and Date: 20110917-20
• [Presentation] AM菌のリン酸吸収・運搬メカニズム (2011)
  • Author(s): 江沢辰広,菊池裕介,土方野分
  • Organizer: 日本土壌肥料学会
  • Place of Presentation: つくば
  • Year and Date: 2011-08-09
  • Invited
• [Presentation] Mycorrhizal formation enhances in planta nifH expression of diazotrophic endophytes and N acquisition in a pioneer grass species Miscanthus sinensis grown in acid sulfate soil (2010)
  • Author(s): Kageyama C., Arakawa R., Nakajima R., Osaki M., and Ezawa T.
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Miyazaki, Japan
  • Year and Date: 20100920-24
• [Presentation] Responses of arbuscular mycorrhizal fungal communities to soil pH : a synthesis of field studies and a model experiment (2010)
  • Author(s): Kawahara A., An G.H., Miyakawa S., Sonoda J., Osaki M., and Ezawa T.
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Miyazaki, Japan
  • Year and Date: 20100920-24
• [Book] 植物の生育を助ける微生物「菌根菌」,環境と微生物の事典,日本微生物生態学会編 (2014)
  • Author(s): 江沢辰広
  • Publisher: 朝倉書店
• [Book] Detection and characterization of mycoviruses in arbuscular mycorrhizal fungi by deep-sequencing, Methods in Molecular Biology, I. Uyeda and C. Masuta Ed., Springer Protocol (2014)
  • Author(s): Ezawa T., Ikeda Y., Shimura H., and Masuta C.
  • Publisher: Humana Press Inc. NJ, USA.
• [Remarks] 成果掲載ホームページ
  • URL: http://www.agr.hokudai.ac.jp/botagr/rhizo/RhizoCont/index.html
• [Patent(Industrial Property Rights)] 法面緑化工法 (2010)
  • Inventor(s): 江沢辰広,大崎満,宮川祥江,田中 淳,堀江直樹,伴資英
  • Industrial Property Rights Holder: 江沢辰広,大崎満,宮川祥江,田中 淳,堀江直樹,伴資英
  • Industrial Property Rights Type: 特許特開2011-250729
  • Patent Publication Number: 特開2011-250729
  • Filing Date: 2010-06-01