2012 Fiscal Year Final Research Report
Studies on molecular mechanisms for functional evolution of bacteria in the environment
| Project/Area Number |
22380047
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tohoku University |
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Principal Investigator |
NAGATA Yuji 東北大学, 大学院・生命科学研究科, 准教授 (30237531)
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| Co-Investigator(Kenkyū-buntansha) |
TSUDA Masataka 東北大学, 大学院・生命科学研究科, 教授 (90172022)
OHTSUBO Yoshiyuki 東北大学, 大学院・生命科学研究科, 助教 (40342761)
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| Research Collaborator |
DAMBORSKY Jiri マサリク大学(チェコ共和国), 理学部, 教授
SENDA Toshiya 産業技術総合研究所, バイオメディシナル情報研究センター, 主任研究員
TANOKURA Masaru 東京大学, 大学院農学生命科学研究科, 教授
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| Project Period (FY) |
2010 – 2012
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| Keywords | 環境細菌 / 自然生態系 / 人為起源物質 / 細菌進化 / 微生物機能開発 |
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| Research Abstract |
To get insights into the functional evolution of bacteria in the environment, bacteria, which degrade man-made chlorinated pesticide γ-HCH, and dehalogenases, which are key enzymes for the degradation of chlorinated compounds, were analyzed in detail. (1) structure-function relationships of some dehalogenases were revealed and their evolution process was suggested, (2) the process of appearance and evolution of γ-HCH-degrading bacteria was proposed on the basis of the structure and function of their genomes and mobile genetic elements, and (3) the system for verifying evolution process of bacteria in the environment was constructed.
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[Journal Article] ParI, an orphan ParA family protein from Pseudomonas putida KT2440-specific genomic island, interferes with the partition system of IncP-7 plasmids2012
Author(s)
Miyakoshi, M., M. Shintani, K. Inoue, T. Terabayashi, F. Sai, M. Ohkuma, H. Nojiri, Y. Nagata, M. Tsuda
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Journal Title
Environ Microbiol
Volume: 14
Pages: 2946-2959
DOI
Peer Reviewed
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[Journal Article] Genomic organization and genomic structural rearrangements of Sphingobium japonicum UT26, an archetypal gamma-hexachlorocyclohexane-degrading bacterium2011
Author(s)
Nagata, Y., S. Natsui, R. Endo, Y. Ohtsubo, N. Ichikawa, A. Ankai, A. Oguchi, S. Fukui, N. Fujita, and M. Tsuda
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Journal Title
Enzyme and Microbial Technology
Volume: 49
Pages: 499-508
DOI
Peer Reviewed
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[Journal Article] Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering2010
Author(s)
Prokop, Z., Y. Sato, J. Brezovsky, T. Mozga, R. Chalooupkova, T. Koudelakova, P. Jerabek, V. Stepankova, R. Natsume, J. G.E. van Leeuwen, D. B. Janssen, J. Florian, Y. Nagata, T. Senda, and J. Damborsky
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Journal Title
Angewandte Chemie
Volume: 49
Pages: 6111-6115
DOI
Peer Reviewed
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[Journal Article] Analysis of extracellular alginate lyase and its gene from a marine bacterial strain, Pseudoalteromonas atlantica AR062010
Author(s)
Matsushima, R., H. Danno, M. Uchida, K. Ishihara, T. Suzuki, M. Kaneniwa, Y. Ohtsubo, Y. Nagata, and M. Tsuda
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Journal Title
Appl Microbiol Biotechnol
Volume: 86
Pages: 567-576
DOI
Peer Reviewed
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