Characterization and modification of molecular mechanism for transcriptional regulation of the degradation enzyme system of an environmental pollutant

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380050
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Nagaoka University of Technology
• Principal Investigator: FUKUDA Masao 長岡技術科学大学, 工学部, 教授 (20134512)
• Co-Investigator(Kenkyū-buntansha): KASAI Daisuke 長岡技術科学大学, 工学部, 助教 (80452085)
• Project Period (FY): 2010 – 2012
• Keywords: 環境微生物

Research Abstract

Rhodococcus jostii RHA1 is a strong degrader of the environmental pollutant, polychlorinated biphenyls (PCBs). Induction of its degradation enzymes requires an inducing substrate and is conducted by a couple of two-component transcriptional regulators, BphS1T1 and BphS2T2. Mechanism of transcriptional regulation of BphS1T1 and BphS2T2 were examined to develop a strain whose PCB-degradation enzymes are induced by PCBs themselves. Phosphorylation of BphT (BphT1/T2) in the presence of the inducing substrate was indicated. A novel 24-bp consensus sequence was shared among the degradation enzyme gene operon promoters and had necessary and sufficient function for transcriptional induction by BphST, suggesting that the 24-bp sequence serves the binding site for the transcriptional complex including BphT.

Research Products

• [Journal Article] Gene cluster and regulation system for 1,1-dichloro-2,2-bis(4-chlorophenyl)ethyl ene (DDE) degradation in Janibacter sp. TYM3221 (2013)
  • Author(s): P.A.T. Nguyen, T.H.T. Trinh, Y. Fukumitsu, J. Shimodaira, K. Miyauchi, M. Tokuda, D. Kasai, E. Masai, M. Fukuda
  • Journal Title: J. Biosci. Bioeng Volume: Vol.116, No.1 Pages: 91-100
  • DOI: DOI:10.1016/j.jbiosc.2013.01.007
  • Peer Reviewed
• [Journal Article] The 24-bp consensus sequence responsible for regulation of the BphS1T1 two-component system in a hybrid promoter (2012)
  • Author(s): J. Shimodaira, Y. Furusawa, Y. Miyazawa, D. Kasai, K. Miyauchi, E. Masai, M. Fukuda
  • Journal Title: J. Biosci. Bioeng Volume: Vol.113 No. 3 Pages: 279-285
  • DOI: DOI:10.1016/j.jbiosc.2011.10.021
  • Peer Reviewed
• [Journal Article] Dual two-component regulatory systems are involved in aromatic compound degradation in a polychlorinated-biphenyl degrader (2010)
  • Author(s): H. Takeda, J. Shimodaira, K. Yukawa, N. Hara, D. Kasai, K. Miyauchi, E. Masai, M.
  • Journal Title: Rhodococcus jostii RHA1, J. Bacteriol. Volume: Vol.192, No.18 Pages: 4741-4751
  • DOI: DOI:10.1128/JB.00429-10
  • Peer Reviewed
• [Presentation] Development of a PCB degrading microorganism (2012)
  • Author(s): M. Fukuda
  • Organizer: 15th International Biotechnology Symposium and Exhibition (IBS 2012)
  • Place of Presentation: Daegu (Korea)
  • Year and Date: 2012-09-18
• [Presentation] Induction mechanism of biphenyl/PCB-degradation pathway in a Rhodococcus degrader (2012)
  • Author(s): M. Fukuda, J. Shimodaira, K. Miyauchi, H. Takeda, D. Kasai, E. Masai
  • Organizer: Environmental Microbiology and Biotechnology in the frame of the Knowledge-Based Bio and Green Economy (EMB2012)
  • Place of Presentation: Bologna(Italy)
  • Year and Date: 2012-04-10
• [Presentation] The essential sequence for transcriptional regulation of biphenyl/ polychlorinated biphenyls-degradation gene promoters in Rhodococcus jostii RHA1 (2011)
  • Author(s): J. Shimodaira, Y. Miyazawa, Y. Furusawa, H. Takeda, D. Kasai, K. Miyauchi, E. Masai, M. Fukuda
  • Organizer: International Union of Microbiological Societies (IUMS 2011)
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-06
• [Presentation] Characterization of 1,1-dichloro-2,2- bis(4-chlorophenyl) ethylene (DDE) degradation enzyme system in the strain Janibacter sp. TYM3221 (2011)
  • Author(s): P.A.T. Nguyen, Y. Sato, T. Iwasaki, K. Miyauchi, D. Kasai, E. Masai, M. Fukuda
  • Organizer: International Union of Microbiological Societies (IUMS2011)
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-06
• [Presentation] Regulatory mechanism of biphenyl/PCB-degradation gene transcription in Rhodococcus jostii RHA1 (2010)
  • Author(s): J. Shimodaira, Y. Miyazawa, Y. Furusawa, H. Takeda, D. Kasai, K. Miyauchi, E. Masai,M. Fukuda
  • Organizer: 14th International Biotechnology Symposium and Exhibition (IBS 2010)
  • Place of Presentation: Rimini (Italy)
  • Year and Date: 2010-09-14
• [Remarks]
  • URL: http://bio.nagaokaut.ac.jp/~fukuda-l/