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2012 Fiscal Year Final Research Report

Molecular circulation and carbon fixation by C1-microorganism consortia

Research Project

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Project/Area Number 22380052
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

SAKAI Yasuyoshi  京都大学, 大学院・農学研究科, 教授 (60202082)

Co-Investigator(Kenkyū-buntansha) YURIMOTO Hiroya  京都大学, 大学院・農学研究科, 准教授 (00283648)
OKU Masahide  京都大学, 大学院・農学研究科, 助教 (10511230)
Project Period (FY) 2010 – 2012
Keywordsメタン / メタノール / メタン酸化菌 / 生育促進 / レドックス / オートファジー / 複合生物系 / メタノール資化性細菌
Research Abstract

In order to strengthen carbon fixation in global carbon cycle, we studied the useful functions of C1-microorganism consortia including methane-and methanol-utilizing microorganisms and molecular mechanism of molecular and energy circulation. We revealed distribution of C1-microorganisms and foundnovel functions necessary for proliferation at phyllosphere.

  • Research Products

    (34 results)

All 2013 2012 2011 2010

All Journal Article (25 results) (of which Peer Reviewed: 20 results) Presentation (8 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Hyper-activation of target of rapamycin (TOR) kinase 1 decreases intracellular glutathione content in Saccharomyces cerevisiaerevealed by LC-MS/MS analysis2013

    • Author(s)
      M. Oku, Y. Ichiki, A. Shiraishi, T. Takahara, T. Maeda,and Y. Sakai
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Dominant colonization and inheritance of Methylobacteriumsp. strain OR01 on perilla plants2013

    • Author(s)
      M. Mizuno, H. Yurimoto, H. Iguchi, A. Tani, and Y. Sakai
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae2013

    • Author(s)
      M. Oku, J. Hoseki, Y. Ichiki, and Y. Sakai.A
    • Journal Title

      FEBS Lett.

      Volume: 587 Pages: 793-798

    • DOI

      doi:10.1016/j.febslet.2013.02.003

    • Peer Reviewed
  • [Journal Article] Primary and secondary metabolism regulates lipolysis in appressoria of Colletotrichum orbiculare2012

    • Author(s)
      M. Asakura, K. Yoshino, A.M.. Hill, Y. Kubo, Y. Sakai, and Y. Takano.
    • Journal Title

      Fungal Genet. Biol

      Volume: 49 Pages: 967-97

    • DOI

      doi:10.1016/j.fgb.2012.08.009

    • Peer Reviewed
  • [Journal Article] 異常を回復する化合物レドックスモジュレーターの探索:Redoxfluorの創薬への利用2012

    • Journal Title

      遺伝子医学MOOK

      Volume: 22 Pages: 158-163

  • [Journal Article] Molecular characterization of the Candida boidinii MIG1and its role in regulation of methanol inducible gene expression.2012

    • Author(s)
      Z. .Zhai, H. Yurimoto, and Y. Sakai
    • Journal Title

      Yeast

      Volume: 29 Pages: 293-301

    • DOI

      doi:10.1002/yea.2909

    • Peer Reviewed
  • [Journal Article] Distribution of methanotrophs in the phyllosphere.2012

    • Author(s)
      H. Iguchi, I. Sato, M. Sakakibara, H. Yurimoto, and Y. Sakai.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 76 Pages: 1580-1583

    • DOI

      doi:10.1271/bbb.120281

    • Peer Reviewed
  • [Journal Article] Distribution of pink-pigmented facultative methylotrophs on leaves of vegetables2012

    • Author(s)
      M. Mizuno, H. Yurimoto, N. Yoshida, H. Iguchi, and Y. Sakai.
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 76 Pages: 578-580

    • DOI

      doi:10.1271/bbb.110737

    • Peer Reviewed
  • [Journal Article] Msn5p is involved in formaldehyde resistance but not in oxidative stress response in the methylotrophic yeast Candida boidinii2012

    • Author(s)
      Z. Zhai, H. Yurimoto, and Y. Sakai
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 76 Pages: 299-304

    • DOI

      doi:10.1271/bbb.110679

    • Peer Reviewed
  • [Journal Article] Assessment of physiological redox state with FRET protein probes. Antioxid.2012

    • Author(s)
      M. Okuand Y. Sakai.
    • Journal Title

      Redox Signal

      Volume: 16 Pages: 698-704

    • DOI

      doi:10.1089/ars.2011.4251

    • Peer Reviewed
  • [Journal Article] Stimulation of methanotrophic growth in co-cultures by cobalamin excreted by rhizobia2011

    • Author(s)
      H. Iguchi, H. Yurimoto, and Y. Sakai.
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 77 Pages: 8509-8515

    • DOI

      doi:10.1128/AEM.05834-11

    • Peer Reviewed
  • [Journal Article] Yeast methylotrophy and autophagy in a methanol-oscillating environment on growing Arabidopsis thalianaleaves2011

    • Author(s)
      K. Kawaguchi, H. Yurimoto, M. Oku, and Y. Sakai
    • Journal Title

      PLoS ONE

      Volume: 6 Pages: e25257

    • DOI

      doi:10.1371/journal.pone.0025257

    • Peer Reviewed
  • [Journal Article] 細胞内の酸化還元状態を検知するタンパク質FRETプローブ, レドックスフロール 酵母の酸化ストレス応答から学び, それを利用する新戦略2011

    • Author(s)
      奥 公秀、阪井康能
    • Journal Title

      化学と生物

      Volume: 49 Pages: 516-517

  • [Journal Article] C1 微生物複合生物系による省エネ型炭素固定技術.2011

    • Author(s)
      阪井康能、由里本博也
    • Journal Title

      環境資源工学

      Volume: 58 Pages: 59-63

  • [Journal Article] Inhibition of surgicaltrauma-enhanced peritoneal dissemination of tumor cells by human catalase derivatives in mice2011

    • Author(s)
      C. Nishizaki, M. Nishikawa, T. Yata, T. Yamada, Y. Takahashi, M. Oku, H. Yurimoto, Y. Sakai,K. Nakanishi, and Y. Takakura
    • Journal Title

      Free Radic. Biol. Med

      Volume: 51 Pages: 773-779

    • DOI

      doi:10.1016/j.freeradbiomed.2011.05.025

    • Peer Reviewed
  • [Journal Article] Yeast methylotrophy: metabolism, gene regulation, and peroxisome homeostasis2011

    • Author(s)
      H. Yurimoto, M. Oku, and Y. Sakai
    • Journal Title

      Int. J. Microbiol.

      Volume: 2011 Pages: 101298

    • DOI

      doi:10.1155/2011/101298

    • Peer Reviewed
  • [Journal Article] Methylovulum miyakonensegen. nov., sp. nov., a novel type I methanotroph from a forest soil2011

    • Author(s)
      H. Iguchi, H. Yurimoto, and Y. Sakai
    • Journal Title

      Int. J. Syst. Evol. Microbiol

      Volume: 61 Pages: 810-815

    • DOI

      doi:10.1099/ijs.0.019604-0

    • Peer Reviewed
  • [Journal Article] Redoxfluorを用いた細胞内酸化還元状態の可視化2011

    • Author(s)
      奥 公秀、阪井康能
    • Journal Title

      実験医学

      Volume: 29 Pages: 945-950

  • [Journal Article] C1微生物と植物の相互作用と炭素循環 ‐代謝生化学・分子細胞生物学からの理解‐2010

    • Author(s)
      由里本博也、阪井康能
    • Journal Title

      植物の生長調節

      Volume: 45 Pages: 125-131

  • [Journal Article] Atg8 regulates vacuolar membrane dynamics in a lipidation-independent manner in Pichia pastoris2010

    • Author(s)
      N. Tamura, M. Oku, and Y. Sakai
    • Journal Title

      J. Cell Sci

      Volume: 123 Pages: 4107-4116

    • DOI

      doi:10.1242/jcs.070045

    • Peer Reviewed
  • [Journal Article] Crystal structureof 3-hexulose- 6-phosphate synthase, a member of the orotidine 5'-monophosphate decarboxylase suprafamily2010

    • Author(s)
      I. Orita, A. Kita, H. Yurimoto, N. Kato, Y. Sakai, and K. Miki
    • Journal Title

      Proteins

      Volume: 78 Pages: 3488-3492

    • DOI

      doi:10.1002/prot.22860

    • Peer Reviewed
  • [Journal Article] Soluble and particulate methane monooxygenase gene clusters in the type I methanotroph Methylovulum miyakonense HT122010

    • Author(s)
      H. Iguchi, H. Yurimoto, and Y. Sakai
    • Journal Title

      FEMS Microbiol. Lett

      Volume: 312 Pages: 71-76

    • DOI

      doi:10.1111/j.1574-6968.2010.02101.x

    • Peer Reviewed
  • [Journal Article] Peroxisomes as dynamic organelles: autophagic degradation2010

    • Author(s)
      Oku, M. and Sakai, Y
    • Journal Title

      FEBS J

      Volume: 277 Pages: 3289-3294

    • DOI

      doi:10.1111/j.1742-4658.2010.07741.x

    • Peer Reviewed
  • [Journal Article] novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomes2010

    • Author(s)
      T. Yano, M. Oku, N. Akeyama, A. Itoyama, H. Yurimoto, S. Kuge, Y. Fujiki, and Y. Sakai.A
    • Journal Title

      Mol. Cell. Biol

      Volume: 30 Pages: 3758-3766

    • DOI

      doi:10.1128/MCB.00121-10

    • Peer Reviewed
  • [Journal Article] Trm2p-dependent derepression is essential for methanol-specific gene activation in the methylotrophic yeast Candida boidinii2010

    • Author(s)
      Y. Sasano, H. Yurimoto, M. Kuriyama, and Y. Sakai
    • Journal Title

      FEMS Yeast Res

      Volume: 10 Pages: 535-544

    • DOI

      doi:10.1111/j.1567-1364.2010.00640.x

    • Peer Reviewed
  • [Presentation] PpAtg18 mediates switch of vacuolar dynamics between micro-and macropexophagy2012

    • Author(s)
      Sakai, Y
    • Organizer
      Gordon Research Conference on Autophagy in Stress, Development and Disease
    • Place of Presentation
      Ventura, USA
    • Year and Date
      20120311-16
  • [Presentation] Regulation and physiological role of autophagy in methylotrophic yeast.2011

    • Author(s)
      Sakai, Y
    • Organizer
      he 2nd Sino-Japan Autophagy Symposium.
    • Place of Presentation
      Kanagawa, Japan
    • Year and Date
      20111005-07
  • [Presentation] C1-metabolism coupled gene expression and molecular ecology at phyllosphere2011

    • Author(s)
      Sakai, Y
    • Organizer
      International Union of Microbilogical Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      20110906-10
  • [Presentation] Methylotrophy and autophagy: from genome and molecules to yeast life style in nature2011

    • Author(s)
      Sakai, Y
    • Organizer
      International Symposium on Microbial Genomic Research
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2011-12-02
  • [Presentation] Regulation and molecular basis for membrane dynamics of micropexophagy.2010

    • Author(s)
      Sakai, Y
    • Organizer
      The 1st Sino-Japan Autophagy Symposium
    • Place of Presentation
      Xi'an, China
    • Year and Date
      20101014-17
  • [Presentation] Regulation and molecular basis for membrane dynamics of micropexophagy2010

    • Author(s)
      Sakai, Y
    • Organizer
      SwissYeast Meeting
    • Place of Presentation
      Fribourg, Switzerland
    • Year and Date
      20100906-07
  • [Presentation] Methane-utilizing microbial consortium established from a forest soil including Methylovulum miyakonense gen. nov., sp. nov., a novel type I methanotroph2010

    • Author(s)
      Sakai, Y
    • Organizer
      Gordon Research Conference on Molecula Basis of Microbial One-Carbon Metabolism
    • Place of Presentation
      Lewiston, USA
    • Year and Date
      20100801-06
  • [Presentation] Recruitment of PI4-kinase from the Golgi apparatus to PAS.2010

    • Author(s)
      Sakai, Y
    • Organizer
      Gordon Research Conference on Autophagy in Stress, Development and Disease
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      20100425-30
  • [Patent(Industrial Property Rights)] メタン酸化菌に対して用いられるメタン酸化活性向上剤、及びその利用2010

    • Inventor(s)
      阪井康能、井口博之、由里本博也
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      特願2010-183287
    • Filing Date
      2010-08-18

URL: 

Published: 2014-08-29  

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