2012 Fiscal Year Final Research Report
Anti-oxidative mechanism mediated by the yeast Mpr1 that acetylates proline catabolism intermediate and its application
| Project/Area Number |
22380061
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
TAKAGI Hiroshi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (50275088)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Nobuyuki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (10273848)
OHTSU Iwao 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (60395655)
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| Co-Investigator(Renkei-kenkyūsha) |
SHINOZAKI Kazuo 独立行政法人理化学研究所, 植物科学研究センター, センター長 (20124216)
HIBI Takao 福井県立大学, 生物資源学部, 教授 (00285181)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 酵母 / アセチル化酵素 / 酸化ストレス / 活性酸素種 / プロリン代謝 |
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| Research Abstract |
We found that the yeast N-acetyltransferase Mpr1 is a novel anti-oxidative enzyme that acetylates the proline catabolism intermediate, which induces the generation of superoxide anions by inhibiting the mitochondrial respiration, and that accelerates arginine synthesis, leading to the arginine-dependent NO production, which confers oxidative stress tolerance on yeast. Tah18 was first identified as the yeast protein involved in NO synthesis. We also analyzed the X-ray crystal structure and the Mpr1 mutants, indicating that Mpr1 has a unique catalytic reaction mechanism and that the catalytic residues are crucial for oxidative stress tolerance of yeast.
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