2012 Fiscal Year Final Research Report
Analysis of impairment and recovery mechanism of intestinal barrier and its application for design of foods with barrier-protectingactivity
| Project/Area Number |
22380076
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Hiroshima University |
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Principal Investigator |
TANABE Soichi 広島大学, 大学院・生物圏科学研究科, 教授 (90272624)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Takuya 広島大学, 大学院・生物圏科学研究科, 講師 (30526695)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 腸管バリア / タイトジャンクション / Th17 / 乳酸菌 / ビフィズス菌 / D-アラニン |
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| Research Abstract |
Caco-2 cells were used as a human intestinal model. Tight-junction (TJ) of the cells was impaired by the addition of cytokine (TNF-alpha). A milk casein-derived peptide, NPWDQ, recovered the impairment by, at least in part, increasing the expression of occludin, one of TJ proteins. Some lactic acid bacteria and bifidobacteria also exerted the barrier-recovering activity. Itwas found that D-alanine in bacterial cell wall played an important role in the barrier-protecting effects of Streptococcus thermophilusin Caco-2 cells. This finding would be applicable for design of foods with barrier-protecting activity. In addition, Bifidobacterium longumameliorated colitis in mice.
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