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2012 Fiscal Year Annual Research Report

魚類の性分化過程と生殖機能の遺伝的・環境的制御メカニズムの解明

Research Project

Project/Area Number 22380109
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

STRUSSMANN C.A.  東京海洋大学, 海洋科学技術研究科, 教授 (10231052)

Project Period (FY) 2010-04-01 – 2013-03-31
Keywords生殖 / 生理 / 魚類 / 性分化 / 温度 / 性決定
Research Abstract

本研究では、遺伝的性決定機構および温度依存的性決定機構を併せ持つ南米原産のパタゴニアペヘレイOdontesthes hatcheriには、抗ミュラー管ホルモン(Anti-Müllerian hormone; amh)遺伝子の雄特異的な重複遺伝子(amhy)が存在することが判明した。さらにその転写産物が性分化時期に特異的に発現すること、本遺伝子の翻訳阻害により雄性分化が阻害され雌性分化が促進されることを発見し、amhyが本種の精巣決定遺伝子であることを実証した。一方、性決定機構が極めて強い温度依存性を示す近縁種のペヘレイOdontesthes bonariensisでは、高温が誘起する雄性分化時に、ストレスホルモンであるコルチゾルと雄性ホルモンである11-ケトテストステロン(11-KT)が上昇することを明らかにした。また、外因性コルチゾルを孵化稚魚に投与したところ、高温刺激なしでも11-KTの代謝酵素遺伝子である11-β水酸基脱水素酵素(hsd11b2)mRNA量および11-KT量が上昇することが明らかとなった。従って、本種では、高温によるコルチゾル量増加によりhsd11b2 mRNA量の上昇が誘起され、結果として11-KT量が増加し雄化が引き起こされると考えられた。さらに、ペヘレイにおいてもamhyホモログの単離に成功し、成魚を用いて本遺伝子と表現型性の関連を調査したところ、amhyは雄の表現型性と強くリンクしていることが明らかとなった。amhyを保持する孵化稚魚を性的に中立な水温で飼育したところ、amhy mRNAは性決定/性分化時期の生殖腺において安定的に発現し、これらの個体は100%雄へと分化したことから、本遺伝子は性決定機構が極めて強い温度依存性を示すペヘレイにおいても雄性分化に重要な役割を果たしていることが明らかとなった。

Current Status of Research Progress
Reason

24年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

24年度が最終年度であるため、記入しない。

  • Research Products

    (16 results)

All 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (13 results) (of which Invited: 2 results)

  • [Journal Article] Genotypic sex determination in teleosts: insights from the testis-determining amhy gene2013

    • Author(s)
      R.S. Hattori, C.A. Strussmann, J.I. Fernandino, and G.M. Somoza
    • Journal Title

      General and Comparative Endocrinology

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Effects of global warming on fish reproductive endocrine axis, with special emphasis in pejerrey Odontesthes bonariensis2013

    • Author(s)
      L.A. Miranda, T. Chalde, M. Elisio, and C.A. Strussmann
    • Journal Title

      General and Comparative Endocrinology

      Volume: in press Pages: in press

    • Peer Reviewed
  • [Journal Article] The cortisol and androgen pathways cross talk in high temperature-induced masculinization: the 11-hydroxysteroid dehydrogenase as a key enzyme2012

    • Author(s)
      J.I. Fernandino, R.S. Hattori, A. Kishii, C.A. Strussmann, and G.M.Somoza
    • Journal Title

      Endocrinology

      Volume: 153 Pages: 6003-6011

    • DOI

      doi: 10.1210/en.2012-1517

    • Peer Reviewed
  • [Presentation] Gradient of amh gene expression during sex differentiation in pejerrey Odontesthes bonariensis2013

    • Author(s)
      M. Sarida, Y. Zhang, Y. Yamamoto, R.S.Hattori, and C.A. Strussmann
    • Organizer
      Annual (Spring) Meeting of the Japanese Society of Fisheries Science
    • Place of Presentation
      Tokyo Japan
    • Year and Date
      20130326-29
  • [Presentation] Genotypic sex determination in pejerrey: evidences from the testis-determining gene amhy2013

    • Author(s)
      Y. Zhang, M. Sarida, R.S. Hattori, Y. Yamamoto, and C.A. Strussmann
    • Organizer
      Annual (Spring) Meeting of the Japanese Society of Fisheries Science
    • Place of Presentation
      Tokyo Japan
    • Year and Date
      20130326-20130329
  • [Presentation] Genetic contribution of two amh paralogues to gonadal sex determination in pejerrey Odontesthes bonariensis2013

    • Author(s)
      Y. Zhang, R.S. Hattori, Y. Yamamoto, and C.A. Strussmann
    • Organizer
      Joint Japan-China-Korea International Symposium on Marine Environment and Energy
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2013-01-19
  • [Presentation] The presence of the sex determining gene amhy in South American silversides and its role on gonadal fate2012

    • Author(s)
      R.S. Hattori, C.A. Strussmann, G.M. Somoza, and L.F. Almeida-Toledo
    • Organizer
      58th Brazilian Congress of Genetics
    • Place of Presentation
      Foz do Iguacu, Brazil
    • Year and Date
      20120911-20120914
  • [Presentation] Temperature effects on sex determination of Chirostoma estor, a freshwater atherinopsid2012

    • Author(s)
      G.A. Coronoa-Herrera, C.C. Martinez-Chavez, R.S. Hattori, C.A. Martinez-Palacios, C.A. Strussmann, and R. Cardenas-Reygadas
    • Organizer
      7th International Symposium on Fish Endocrinology
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      20120901-20120906
  • [Presentation] High temperature inhibits cyp19a1a expression and estradiol synthesis in pejerrey (Odontesthes bonariensis) ovaries in vitro2012

    • Author(s)
      L.A. Miranda, M. Elisio, and C.A. Strussmann
    • Organizer
      7th International Symposium on Fish Endocrinology
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      20120901-20120906
  • [Presentation] The cortisol and androgen pathways cross-talk in high temperature induced masculinization: 11B-hydroxysteroid dehydrogenase as a key enzyme2012

    • Author(s)
      J.I. Fernandino, R.S. Hattori, A. Kishii, C.A. Strussmann, and G.M. Somoza
    • Organizer
      7th International Symposium on Fish Endocrinology
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      20120901-20120906
    • Invited
  • [Presentation] Y chromosome-linked amhy gene and sex determination of atherinopsid species2012

    • Author(s)
      R.S. Hattori, Y. Murai, M. Oura, S. Masuda, S.K. Majhi, T. Sakamoto, J.I. Fernandino, G.M. Somoza, L. Almeida-Toledo, and C.A. Strussmann
    • Organizer
      7th International Symposium on Fish Endocrinology
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      20120901-20120906
    • Invited
  • [Presentation] Expression profile of two amh homologues during gonadal development in pejerrey Odontesthes bonariensis2012

    • Author(s)
      Y. Yamamoto, Y. Zhang, M. Sarida, R.S. Hattori, M. Yokota, andC.A. Strussmann
    • Organizer
      7th International Symposium on Fish Endocrinology
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      20120901-20120906
  • [Presentation] Temperature-dependent sex determination and physiology of gonadal sex differentiation in pejerrey Odontesthes bonariensis2012

    • Author(s)
      G.M. Somoza, J.I. Fernandino, R.S. Hattori, and C.A. Strussmann
    • Organizer
      III International Congress on Inland Water Aquaculture
    • Place of Presentation
      Sangolqui, Ecuador
    • Year and Date
      20120711-12
  • [Presentation] Effects of environmental factors on the elemental composition of pejerrey Odontesthes bonariensis otoliths2012

    • Author(s)
      Y. Wang, D.C. Colautti, G.E. Berasain, C.A.M. Velasco, M. Yokota, and C.A. Strussmann
    • Organizer
      6th World Fisheries Congress
    • Place of Presentation
      Edinburgh, Scotland
    • Year and Date
      20120507-11
  • [Presentation] Temperature effects on sex ratios of pejerrey Odontesthes bonariensis, a fish with temperature-dependent sex determination, in Kasumigaura Lake, Japan2012

    • Author(s)
      C.A. Strussmann, T. Oikawa, T. Otake, S.Kasuga, Y. Wang, and M. Yokota
    • Organizer
      6th World Fisheries Congress
    • Place of Presentation
      Edinburgh, Scotland
    • Year and Date
      20120507-11
  • [Presentation] Characterization and expression profile of rev1 gene during heat stress in medaka (Oryzias latipes)2012

    • Author(s)
      C.A. Strussmann, P.A. Yu, R.S. Hattori, and M. Yokota
    • Organizer
      58th Brazilian Congress of Genetics
    • Place of Presentation
      Foz do Iguacu, Brazil
    • Year and Date
      20120111-14

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Published: 2014-07-24  

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