Mechanisms of genetic and environmental control of gonadal sex differentiation and reproductive function in fish

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: STRUSSMANN C.a. 東京海洋大学, 海洋科学技術研究科, 教授 (10231052)
• Project Period (FY): 2010 – 2012
• Keywords: 環境 / 温度 / 魚類 / 遺伝 / 生殖 / 性分化 / 性決定 / ストレス

Research Abstract

This study examined how water temperature modulates the expression dynamics of several genes related to gonadal sex differentiation and reproductive function of fish, the process and effects of heat-induced gonadal apoptosis, the process and effects of environment-induced stress on gonadal sex determination, and the roles of the central nervous system in temperature-dependent gonadal sex determination using medaka and atheriniform fishes as models. This study provides novel and crucial insights on the molecular, biochemical, and physiological pathways of action of water temperature on the processes of gonadal sex differentiation and reproductive function of fish.

Research Products

• [Journal Article] Effects of global warming on fish reproductive endocrine axis, with special emphasis in pejerrey Odontesthes bonariensis. (2013)
  • Author(s): L.A. Miranda, T. Chalde, M. Elisio, and C.A.Strussmann.
  • Journal Title: General and ComparativeEndocrinology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Genotypic sex determination in teleosts: insights from the testis-determining amhy gene (2013)
  • Author(s): R.S. Hattori, C.A. Strussmann, J.I. Fernandino, and G.M. Somoza
  • Journal Title: General and Comparative Endocrinology Volume: (in press)
  • Peer Reviewed
• [Journal Article] A Y-linked anti-Mullerian hormone duplication takes over a critical role in sex determination (2012)
  • Author(s): R.S. Hattori, Y. Murai, M. Oura, S. Masuda, S.K. Majhi, T. Sakamoto, J.I. Fernandino, G.M. Somoza, M. Yokota, and C.A. Strussmann. 2012.
  • Journal Title: Proceedings of the National Academy of Sciences USA Volume: 109 Pages: 2955-2959
  • DOI: doi:10.1073/pnas.1018392109
  • Peer Reviewed
• [Journal Article] The cortisol and androgen pathways cross talk in high temperature-induced masculinization (2012)
  • Author(s): J.I. Fernandino, R.S. Hattori, A. Kishii, C.A.Strussmann, and G.M.Somoza
  • Journal Title: the 11-hydroxysteroid dehydrogenase as a key enzyme. Endocrinology Volume: 153 Pages: 6003-6011
  • DOI: doi:10.1210/en.2012-1517
  • Peer Reviewed
• [Journal Article] Temperature effects on sex differentiation of the reciprocal hybrids of Odontesthes bonariensis and O. hatcheri (Atherinopsidae) (2011)
  • Author(s): J. Inazawa, R.S. Hattori, M. Oura, M. Yokota and C.A. Strussmann
  • Journal Title: Aquaculture Research Volume: 42 Pages: 746-753
  • DOI: doi:10.1111/j.1365-2109.2010.02757.x
  • Peer Reviewed
• [Journal Article] Analysis of sexually dimorphic expression of genes at early gonadogenesis of pejerrey Odontesthes bonariensis using a heterologous microarray (2011)
  • Author(s): J.I. Fernandino, J.T. Popesku, B.Paul-Prasanth, H. Xiong, R.S. Hattori, M. Oura, C.A. Strussmann, G.M. Somoza, M. Matsuda, Y. Nagahama, V.L. Trudeau
  • Journal Title: Sexual Development Volume: 5 Pages: 89-101
  • DOI: doi:10.1159/000324423
  • Peer Reviewed
• [Journal Article] Extrahypophyseal expression of gonadotropin subunits in pejerrey Odontesthes bonariensis and effects of high water temperatures on their expression (2011)
  • Author(s): M. Elisio, F.N. Soria, J.I. Fernandino, C.A.Strussmann, G.M. Somoza, and L.A. Miranda
  • Journal Title: General and Comparative Endocrinology Volume: 175 Pages: 329-336
  • DOI: doi:10.1016/j.ygcen.2011.11.036
  • Peer Reviewed
• [Journal Article] Establishment of a strain inheriting a sex-linked SNP marker in Patagonianpejerrey (Odontesthes hatcheri), a species with both genotypic and temperature- dependent sex determination (2010)
  • Author(s): R.S. Hattori, M. Oura, T. Sakamoto, M.Yokota, S. Watanabe, and C.A. Strussmann
  • Journal Title: Animal Genetics Volume: 41 Pages: 81-84
  • DOI: doi:10.1111/j.1365-2052.2009.01948.x
  • Peer Reviewed
• [Journal Article] Construction of a genetic map and development of DNA markers linked to the sex determining locus in the Patagonian pejerrey (Odontesthes hatcheri) (2010)
  • Author(s): E. Koshimizu, C.A. Strussmann, N. Okamoto, H. Fukuda, and T. Sakamoto
  • Journal Title: Marine Biotechnology Volume: 12 Pages: 8-13
  • DOI: doi:10.1007/s10126-009-9194-1
  • Peer Reviewed
• [Journal Article] Molecular characterization of cyp11a1 and cyp11b1 and their gene expression profile in pejerrey during early gonadal development (2010)
  • Author(s): M. Blasco, J.I. Fernandino, L.G. Guilgur, C.A. Strussmann, G.M. Somoza, and D. Vizziano-Cantonnet
  • Journal Title: Comparative Biochemistry and Physiology A (Molecular and Integrative Physiology) Volume: 156 Pages: 110-118
  • DOI: doi:10.1016/j.cbpa.2010.01.006
  • Peer Reviewed
• [Journal Article] Molecular cloning and expression analysis of Fshr and Lhr in relation to Fshb and Lhb subunits during the period of temperature-dependent sex determination in pejerrey Odontesthes bonariensis (2010)
  • Author(s): T. Shinoda, L.A. Miranda, K. Okuma, R.S.Hattori, J.I. Fernandino, G. Yoshizaki, G.M. Somoza, and C.A. Strussmann
  • Journal Title: Molecular Reproduction and Development Volume: 77 Pages: 521-532
  • DOI: doi:10.1002/mrd.21179
  • Peer Reviewed
• [Journal Article] Implications of climate change for the reproductive capacity and survival of Atherinopsid fish species (2010)
  • Author(s): C.A. Strussmann, D.O. Conover, G.M. Somoza, and L.A. Miranda
  • Journal Title: Journal of Fish Biology Volume: 77 Pages: 1818-1834
  • DOI: doi:10.1111/j.1095-8649.2010.02780.x1
  • Peer Reviewed
• [Journal Article] Vulnerability of silverside (Odontesthes bonariensis) populations to climate change in pampean lakes of Argentina (2010)
  • Author(s): G.A. Kopprio, R.H. Freije, C.A. Strussmann, G. Kattner, M.S. Hoffmeyer, C.A. Popovich, and R.J. Lara
  • Journal Title: Journal of Fish Biology Volume: 77 Pages: 1856-1866
  • DOI: doi:10.1111/j.1095-8649.2010.02750.x
  • Peer Reviewed
• [Journal Article] Thermal and endocrine regulation of gonadal apoptosis during sex differentiation inpejerrey Odontesthes bonariensis
  • Author(s): Y. Yamamoto, R.S. Hattori, A. Kitahara, H.Kimura, M. Yamashita, and C.A. Strussmann
  • Journal Title: SexualDevelopment Volume: (in press)
  • Peer Reviewed
• [Journal Article] Environmental stress-induced testis differentiation
  • Author(s): J.I. Fernandino, R.S. Hattori, O.D. Moreno Acosta, C.A. Strussmann, G.M. Somoza
  • Journal Title: androgen as a by-product of cortisol inactivation. General and ComparativeEndocrinology Volume: (in press)
  • Peer Reviewed
• [Presentation] Genotypic sex determination in pejerrey (2013)
  • Author(s): Y. Zhang, M. Sarida, R.S. Hattori, Y.Yamamoto, and C.A. Strussmann
  • Organizer: evidencesfrom the testis-determining gene amhy. Annual (Spring) Meeting of the Japanese Society of Fisheries Science
  • Place of Presentation: Tokyo Japan
  • Year and Date: 20130326-29
• [Presentation] Gradient of amh gene expression during sex differentiation in pejerrey Odontesthes bonariensis (2013)
  • Author(s): M. Sarida, Y. Zhang, Y. Yamamoto, R.S.Hattori, and C.A. Strussmann
  • Organizer: Annual (Spring) Meeting of the Japanese Society of Fisheries Science
  • Place of Presentation: Tokyo Japan
  • Year and Date: 20130326-29
• [Presentation] Genetic contribution of two amh paralogues to gonadal sex determination in pejerrey Odontesthes bonariensis (2013)
  • Author(s): Y. Zhang, R.S. Hattori, Y. Yamamoto, and C.A. Strussmann
  • Organizer: Joint Japan-China-Korea International Symposium on Marine Environment and Energy
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2013-01-19
• [Presentation] Almeida-Toledo, The presence of the sex determining gene amhy in South American silversides and its role on gonadal fate. (2012)
  • Author(s): R.S. Hattori, C.A. Strussmann, G.M. Somoza, and L.F
  • Organizer: 58th Brazilian Congress of Genetics
  • Place of Presentation: Foz do Iguacu, Brazil
  • Year and Date: 20120911-14
• [Presentation] Temperature-dependent sex determination and physiology of gonadal sex differentiation in pejerrey Odontesthes bonariensis (2012)
  • Author(s): G.M. Somoza, J.I. Fernandino, R.S. Hattori, and C.A. Strussmann
  • Organizer: III International Congress on Inland Water Aquaculture
  • Place of Presentation: Sangolqui, Ecuador
  • Year and Date: 20120711-12
• [Presentation] Temperature effects on the sex determination of the Patzcuaro pez blanco Menidia estor (Jordan, 1880) (2012)
  • Author(s): G.A. Coronoa-Herrera, C.C. Martinez-Chavez, R.S. Hattori, C.A. Martinez-Palacios, C.A. Strussmann, and R. Cardenas-Reygadas
  • Organizer: 3rd Academic Symposium on Biological and Agricultural Sciences
  • Place of Presentation: Morelia, Mexico
  • Year and Date: 20120529-31
• [Presentation] Effects of environmental factors on the elemental composition of pejerrey Odontesthes bonariensis otoliths (2012)
  • Author(s): Y. Wang, D.C. Colautti, G.E. Berasain, C.A.M. Velasco, M. Yokota, and C.A. Strussmann
  • Organizer: 6th World Fisheries Congress
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 20120507-11
• [Presentation] Temperature effects on sex ratios of pejerrey Odontesthes bonariensis, a fish with temperature-dependent sex determination, in Kasumigaura Lake, Japan (2012)
  • Author(s): C.A. Strussmann, T. Oikawa, T. Otake, S.Kasuga, Y. Wang, and M. Yokota
  • Organizer: 6th World Fisheries Congress
  • Place of Presentation: Edinburgh, Scotland
  • Year and Date: 20120507-11
• [Presentation] Characterization and expression profile of rev1 gene during heat stress in medaka (Oryzias latipes) (2012)
  • Author(s): C.A. Strussmann, P.A. Yu, R.S. Hattori, and M. Yokota
  • Organizer: 58th Brazilian Congress of Genetics
  • Place of Presentation: Foz do Iguacu, Brazil
  • Year and Date: 20120111-14
• [Presentation] Temperature effects on sex determination of Chirostoma estor, a freshwater atherinopsid. (2012)
  • Author(s): G.A. Coronoa-Herrera, C.C. Martinez-Chavez, R.S. Hattori, C.A. Martinez-Palacios, C.A. Strussmann, and R. Cardenas-Reygadas.
  • Organizer: 7th International Symposium on FishEndocrinology
  • Place of Presentation: Buenos Aires, Argentina. Abstracts
  • Year and Date: 20120101-06
• [Presentation] High temperature inhibits cyp19a1a expression and estradiol synthesis in pejerrey (Odontesthes bonariensis) ovaries in vitro (2012)
  • Author(s): L.A. Miranda, M. Elisio, and C.A. Strussmann
  • Organizer: 7th International Symposium on Fish Endocrinology
  • Place of Presentation: Buenos Aires, Argentina. Abstracts
  • Year and Date: 20120101-06
• [Presentation] The cortisol and androgen pathways cross-talk in high temperature induced masculinization:11B-hydroxysteroid dehydrogenase as a key enzyme (2012)
  • Author(s): J.I. Fernandino, R.S. Hattori, A. Kishii, C.A.Strussmann, and G.M. Somoza
  • Organizer: 7th International Symposium onFish Endocrinology
  • Place of Presentation: Buenos Aires, Argentina. Abstracts
  • Year and Date: 20120101-06
• [Presentation] Y chromosome-linked amhy gene and sex determination of atherinopsid species (2012)
  • Author(s): R.S. Hattori, Y. Murai, M. Oura, S. Masuda, S.K. Majhi, T. Sakamoto, J.I. Fernandino, G.M. Somoza, L. Almeida-Toledo, and C.A. Strussmann
  • Organizer: 7th International Symposium on Fish Endocrinology
  • Place of Presentation: Buenos Aires, Argentina. Abstracts
  • Year and Date: 20120101-06
• [Presentation] Expression profile of two amh homologues during gonadal development in pejerrey Odontesthes bonariensis (2012)
  • Author(s): Y. Yamamoto, Y. Zhang, M. Sarida, R.S.Hattori, M. Yokota, and C.A. Strussmann
  • Organizer: 7th International Symposium on Fish Endocrinology
  • Place of Presentation: Buenos Aires, Argentina. Abstracts
  • Year and Date: 20120101-06
• [Presentation] Molecular, endocrine, and environmental mechanisms of gonadal sex differentiation in fish and application for sex control (2011)
  • Author(s): C.A. Strussmann
  • Organizer: Japan-China International Forum ofAdvanced Research on Biotechnology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20111110-11
• [Presentation] A duplicated, Y-linked copy of the anti-Mullerian gene determines testicular formation in Patagonian pejerrey Odontesthes hatcheri (2011)
  • Author(s): R.S. Hattori, Y. Murai, M. Oura, S. Masuda, S.K. Majhi, T. Sakamoto, J.I. Fernandino, G.M. Somoza, M. Yokota, and C.A. Strussmann
  • Organizer: 9th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Cochin, India
  • Year and Date: 20110809-14
• [Presentation] Some like it hot: temperature selection during the critical period of thermolabile sex determination in pejerrey (Odontesthes bonariensis) (2011)
  • Author(s): J. Kaiga, C.A. Strussmann, R.S. Hattori, M.Oura, and M. Yokota
  • Organizer: 9th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Cochin, India
  • Year and Date: 20110809-14
• [Presentation] Temperature effects on the sex determination of the Patzcuaro pez blanco Menidia estor (Jordan, 1880) (2011)
  • Author(s): G.A. Coronoa-Herrera, C.C. Martinez-Chavez, R.S. Hattori, C.A. Martinez-Palacios, and C.A. Strussmann
  • Organizer: 2nd Academic Symposium on Biological and Agricultural Sciences
  • Place of Presentation: Morelia, Mexico
  • Year and Date: 20110518-20
• [Presentation] Expression of 11β-hsd2 during temperature- dependent masculinisation in pejerrey Odontesthes bonariensis (2010)
  • Author(s): J.I. Fernandino, R.S. Hattori, A. Kishii, H.Kamioka, C.A. Strussmann and G.M. Somoza
  • Organizer: XII Jornadas Anuales de la Sociedad Argentina de Biologia
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 20101201-03
• [Presentation] Vulnerability of silverside (Odontesthes bonariensis) populations to climate change in pampean lakes of Argentina (2010)
  • Author(s): G.A. Kopprio, R.H. Freije, C.A. Strussmann, G. Kattner, M.S. Hoffmeyer, C.A. Popovich, and R.J. Lara
  • Organizer: Fish and Climate Change 2010 - Annual Meeting of the Fisheries Society of the British Isles 2010
  • Place of Presentation: BelfastNorth Ireland
  • Year and Date: 20100726-30
• [Presentation] Implications of climate change for the reproductive capacity and survival of Atherinopsid fish species (2010)
  • Author(s): C.A. Strussmann, D.O. Conover, G.M.Somoza, and L.A. Miranda
  • Organizer: Fish and Climate Change 2010-Annual Meeting of the Fisheries Society of the British Isles 2010
  • Place of Presentation: Belfast, North Ireland
  • Year and Date: 20100726-30
• [Presentation] Characterization and expression profile of Rev1 gene during heat stress in medaka (Oryzias latipes) (2010)
  • Author(s): A.M.H.B. Tareque, P.G. Yu, R.S. Hattori, M.Yokota, and C.A. Strussmann
  • Organizer: Fish and Climate Change 2010 - Annual Meeting of the Fisheries Society of the British Isles2010
  • Place of Presentation: Belfast, NorthIreland
  • Year and Date: 20100726-30
• [Presentation] Some like it hot: temperature selection during the critical period of thermolabile sex determination in pejerrey (Odontesthes bonariensis) (2010)
  • Author(s): J. Kaiga, C.A. Strussmann, R.S. Hattori, M.Oura and M. Yokota
  • Organizer: Fish and Climate Change 2010 - Annual Meeting of the Fisheries Society of the British Isles2010
  • Place of Presentation: Belfast,NorthIreland
  • Year and Date: 20100726-30
• [Presentation] Use of otolith microchemistry in the field assessment of the effects of water temperature on fish sex determination (2010)
  • Author(s): C.A. Strussmann, T. Oikawa and T. Otake
  • Organizer: Fish and Climate Change 2010 - Annual Meeting of the Fisheries Society of the British Isles2010
  • Place of Presentation: Belfast, NorthIreland
  • Year and Date: 20100726-30