Molecular dynamics to restore/remodel neuromuscular connections during muscle growth and regeneration

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380145
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Kyushu University
• Principal Investigator: TATSUMI Ryuichi 九州大学, 大学院・農学研究院, 准教授 (40250493)
• Co-Investigator(Kenkyū-buntansha): FURUSE Mitsuhiro 九州大学, 大学院・農学研究院, 教授 (30209176) ; MIZUNOYA Wataru 九州大学, 大学院・農学研究院, 助教 (20404056)
• Research Collaborator: RONALD E Allen 米国アリゾナ大学, 教授 ; JUDY E Anderson 加国マニトバ大学, 理学部, 教授
• Project Period (FY): 2010 – 2012
• Keywords: 食肉 / 骨格筋 / 筋幹細胞 / 運動神経 / 神経軸索成長ガイダンス因子Sema3A / 肝細胞増殖因子(HGF) / 塩基性線維芽細胞増殖因子(FGF2) / トランスフォーミング増殖因子(TGF)-β

Research Abstract

Successful regeneration and remodeling of the neuromuscular connections are critical for restoring functional properties of contractile physiology of muscle fibers. While the spatiotemporal regulatory mechanisms coordinating these processes with myogenesis itself remain unclear, various neural factors including attractive and repulsive axon-guidance cue ligands and their receptors may be involved. Recently, we found that satellite cells, resident myogenic stem cells normally positioned beneath the basal lamina of mature muscle fibers, up-regulate a secreted neural chemorepellent semaphorin 3A (Sema3A) during early-differentiation period, upon acute muscle injury in vivo(Tatsumi et al., 2009). The current study encouraged the possible regulation of Sema3A expression by time-coordinated prevalence of extracellular up-regulator (HGF and FGF2) and down-regulator (TGF-β3) concentrations bathing differentiating satellite cells (myoblasts) in regenerating muscle tissue. By understanding this growth factor ligand-driving scenario, we will be able to design new procedures that specifically target regenerative motoneuritogenesis to ensure muscle growth and repair, contributing to the meat-animal production and human sports and health sciences aimed to enhance physical performance and medical therapies for neuromuscular disorders and age-related muscle atrophy

Research Products

• [Journal Article] 衛星細胞による運動神経支配の再構築制御仮説 生体の科学 (金原一郎記念医学医療振興財団・医学書院) (2013)
  • Author(s): 辰巳隆一
  • Journal Title: 特集「特殊な幹細胞としての骨格筋サテライト細胞」 Volume: 第64巻 2号 Pages: 122-131
  • URL: http://www.igaku-shoin.co.jp/journalPortal.do?journalPortalId=425
  • Peer Reviewed
• [Journal Article] Satellite cells produce neural chemorepellent semaphorin 3A upon muscle injury (2013)
  • Author(s): Sato, Y., Do, M.-K. Q.,Suzuki, T., Ohtsubo, H., Mizunoya, W., Nakamura, M., Furuse, M., Ikeuchi, Y., Anderson, J. E.and Tatsumi, R
  • Journal Title: Animal Science Journal Volume: 84 Pages: 185-189
  • DOI: DOI:10.1111/asj.12014
  • Peer Reviewed
• [Journal Article] Comparative analysis of semaphorin 3A in soleus and EDL muscle satellite cells in vitro toward understanding its role in modulating myogenin expression (2013)
  • Author(s): Suzuki, T., Do, M.-K. Q., Sato, U., Ojima, K., Hara, M., Mizunoya, W., Nakamura, M., Furuse, M., Ikeuchi, Y., Anderson, J. E.and Tatsumi, R
  • Journal Title: International Journal of Biochemistry and Cell Biology Volume: 45 Pages: 476-482
  • DOI: DOI:10.1016/j.biocel.2012.10.003
  • Peer Reviewed
• [Journal Article] Time-coordinated prevalence of extracellular HGF, FGF2 and TGF-β3 in crush-injured skeletal muscle (2012)
  • Author(s): Do, M.-K. Q., Suzuki, T., Gerelt, B., Sato, Y., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Tatsumi, R
  • Journal Title: Animal Science Journal Volume: 83 Pages: 712-717
  • DOI: DOI:10.1111/j.1740-0929.2012.01057
  • Peer Reviewed
• [Journal Article] Calcium influx through a possible coupling of cation-channels impacts skeletal muscle satellite cell activation in response to mechanical stretch (2012)
  • Author(s): Hara, M., Tabata, K., Suzuki, T., Do, M.-K. Q., Mizunoya, W., Nakamura, M., Nishimura., S., Tabata, S., Ikeuchi, Y., Sunagawa, K., Anderson, J. E., Allen, R. E., and Tatsumi, R.
  • Journal Title: American Journal of Physiology-Cell Physiology Volume: 302 Pages: C1741-C1750
  • DOI: DOI:10.1152/ajpcell.00068.2012
  • Peer Reviewed
• [Journal Article] Growth factor regulation of neural chemorepellent Sema3A expression in satellite cell cultures (2011)
  • Author(s): Do, M.-K. Q., Sato, Y., Shimizu, N., Suzuki, T., Shono, J.-I., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Tatsumi, R
  • Journal Title: American Journal of Physiology-Cell Physiology Volume: 301 Pages: C1270-C1279
  • DOI: DOI:10.1152/ajpcell.00257.2011
  • Peer Reviewed
• [Journal Article] In vitromeasurement of postnatal changes in proliferating satellite cell frequency during rat muscle growth (2011)
  • Author(s): Suzuki, T., Takaishi, H., Sakata, T., Do, M. Q., Hara, M., Sato, A., Mizunoya, W., Nishimura, T, Hattori, A., Ikeuchi, Y., and Tatsumi, R
  • Journal Title: Animal Science Journal Volume: 81 Pages: 245-251
  • DOI: DOI:10.1111/j.1740-0929.2009.00734.x
  • Peer Reviewed
• [Journal Article] Mechano-biology of skeletal muscle hypertrophy and regeneration: possible mechanism of stretch-induced activation of resident myogenic stem cells (2010)
  • Author(s): Tatsumi, R
  • Journal Title: Animal Science Journal Volume: 81 Pages: 11-20
  • DOI: DOI:10.1111/j.1740-0929.2009.00712.x
  • Peer Reviewed
• [Presentation] 筋幹細胞による筋線維型の自律制御機構 (2013)
  • Author(s): 鈴木貴弘・尾嶋孝一・水野谷航・中村真子・辰巳隆一・池内義秀
  • Organizer: 第116回日本畜産学会大会
  • Place of Presentation: 安田女子大学、広島市
  • Year and Date: 20130927-0330
• [Presentation] Syndecan-3 may implicate in regulation of neural chemorepellent Sema3A expression in satellite cells (2013)
  • Author(s): ド・マイコイ・水野谷航・中村真子・辰巳隆一・池内義秀
  • Organizer: 第116回日本畜産学会大会
  • Place of Presentation: 安田女子大学、広島市
  • Year and Date: 20130327-0330
• [Presentation] 肥大・再生における筋幹細胞・運動神経末端・マクロファージのクロストークダイナミクス(仮題) (2013)
  • Author(s): 辰巳隆一
  • Organizer: 平成25年度日本栄養・食糧学会北海道支部会シンポジウム「組織の機能を支える細胞間クロストーク」
  • Place of Presentation: 北海道大学大学院農学研究院, 北海道札幌市
  • Year and Date: 2013-10-26
• [Presentation] 筋幹細胞のプログラムド・メカノバイオロジー (2012)
  • Author(s): 辰巳隆一
  • Organizer: 第35回日本分子生物学会年会ワークショップ「骨格筋幹細胞研究の最前線」
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡, 福岡市
  • Year and Date: 20121211-1214
• [Presentation] Heparan/chondroitin sulfate chains may mediate HGF/FGF2-induced up-regulation of neural chemorepellent Sema3A in satellite cell cultures (2012)
  • Author(s): Do, M.Q., Shimizu, N., Suzuki, T., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J.E.,and Tatsumi, R
  • Organizer: 2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
  • Place of Presentation: Barga, Lucca, Italy
  • Year and Date: 20120812-0817
• [Presentation] differentiation of satellite cells (2012)
  • Author(s): Suzuki, T., Ojima, K., Do, M.Q., Hara, M., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J.E., and Tatsumi, R
  • Organizer: 2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
  • Place of Presentation: Barga, Lucca, Italy
  • Year and Date: 20120812-0817
• [Presentation] Mechano-sensing calcium-influx machinery that instigates skeletal muscle satellite cell activation (2012)
  • Author(s): Tatsumi, R., Hara, M., Tabata, K., Suzuki, T., Do, M.-K. Q., Mizunoya, W., Nakamura, M., Ikeuchi, Y., Anderson, J. E., and Allen, R. E
  • Organizer: 2012 FASEB Science Research Conference on "Skeletal Muscle Satellite & Stem Cells"
  • Place of Presentation: Barga, Lucca, Italy
  • Year and Date: 20120812-0817
• [Presentation] Studies on signaling receptors responsible for growth factor- regulated Sema3A expression in satellite cells (2012)
  • Author(s): ド・マイコイ・清水直美・鈴木貴弘・水野谷航・中村真子・辰巳隆一・池内義秀
  • Organizer: 第115回日本畜産学会大会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Year and Date: 20120327-0330
• [Presentation] semaphorin 3Aは筋幹細胞の初期分化を制御する (2012)
  • Author(s): 鈴木貴弘・尾嶋孝一・佐藤祐介・中村真子・水野谷航・辰巳隆一・池内義秀
  • Organizer: 第115回日本畜産学会大会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Year and Date: 20120327-0330
• [Presentation] 筋幹細胞による筋再生制御ダイナミクス (2012)
  • Author(s): 辰巳隆一
  • Organizer: 第245回川崎医学会講演会
  • Place of Presentation: 川崎医科大学図書館小講堂,倉敷市
  • Year and Date: 2012-12-12
• [Presentation] 筋幹細胞・運動神経軸索・活性化マクロファージのクロストーク (2012)
  • Author(s): 辰巳隆一
  • Organizer: 北大食肉科学セミナー「骨格筋における異種細胞間コミュニケーションに関する研究展開」
  • Place of Presentation: 北海道大学大学院農学研究院, 北海道札幌市
  • Year and Date: 2012-05-11
• [Presentation] 筋肥大・再生における筋幹細胞・運動神経末端・マクロファージのコミュニケーションに関する研究展開 (2012)
  • Author(s): 辰巳隆一
  • Organizer: 農業・食品産業技術総合研究機構 畜産草地研究所セミナー
  • Place of Presentation: 畜産草地研究所, 茨城県つくば市
  • Year and Date: 2012-03-01
• [Presentation] 筋再生の過程で筋組織幹細胞(衛星細胞)は運動神経末端の再接着制御に関与しているかもしれない:分化期の衛星細胞は神経軸索ガイダンス因子Sema3Aを合成・分泌する (2011)
  • Author(s): 辰巳隆一・Mai-Khoi Q. DO・佐藤祐介・三小田よりこ・清水直美・鈴木貴弘・水野谷航・中村まこ・池内義秀・Judy E. ANDERSON・Ronald E. ALLEN
  • Organizer: 精神・神経疾患研究開発費「筋ジストロフィーに対するトランスレーショナル・リサーチ」の班会議
  • Place of Presentation: JA共済ビル カンファエレンス・ホール, 東京都千代田区
  • Year and Date: 20111201-1202
• [Presentation] 筋幹細胞による運動神経末端の再接着制御 (2011)
  • Author(s): 辰巳隆一
  • Organizer: 第26回日本整形外科学会基礎学術集会パネルディスカッション「骨格筋の変性と再生」
  • Place of Presentation: ベイシア文化ホール(群馬県民会館)および前橋商工会議所, 群馬県前橋市
  • Year and Date: 20111020-1121
• [Presentation] TGF-β2,3は衛星細胞のSema3A発現調節に関与する (2011)
  • Author(s): ド・マイコイ・清水直美・佐藤祐介・水野谷航・中村真子・辰巳隆一・池内義秀
  • Organizer: 第114回日本畜産学会大会
  • Place of Presentation: 北里大学獣医学部十和田キャンパス
  • Year and Date: 20110826-0827
• [Presentation] 筋幹細胞が分泌するsemaphorin 3Aの生理機能 (2011)
  • Author(s): 鈴木貴弘・尾嶋孝一・佐藤祐介・水野谷航・中村真子・辰巳隆一・池内義秀
  • Organizer: 第114回日本畜産学会大会
  • Place of Presentation: 北里大学獣医学部十和田キャンパス
  • Year and Date: 20110826-0827
• [Presentation] 筋細胞におけるsemaphorin3Aの機能 (2011)
  • Author(s): 鈴木貴弘・佐藤祐介・原美菜子・水野谷航・辰巳隆一・池内義秀
  • Organizer: 第113回日本畜産学会大会
  • Place of Presentation: 東京農業大学厚木キャンパス
  • Year and Date: 20110327-0330
• [Presentation] TGF-β2とβ3は衛星細胞のSema3A発現を抑制する (2011)
  • Author(s): ド・マイコイ・佐藤祐介・清水直美・水野谷航・辰巳隆一・池内義秀
  • Organizer: 第113回日本畜産学会大会
  • Place of Presentation: 東京農業大学厚木キャンパス
  • Year and Date: 20110327-0330
• [Presentation] 筋肥大・再生における衛星細胞の役割:衛星細胞はmyogenesisとmoto-neurito- genesisに関与する (2011)
  • Author(s): 辰巳隆一・水野谷航
  • Organizer: 花王(株)生物科学研究所講演会
  • Place of Presentation: 栃木県芳賀郡 市貝町赤羽
  • Year and Date: 2011-09-29
• [Presentation] Mechano-biology of resident myogenic stem cells: implication in postnatal myogenesis and intramuscular motoneuritogenesis (2010)
  • Author(s): Tatsumi, R.,Mizunoya, W., Ikeuchi, Y., Hattori, A., Anderson, J. E., and Allen, R. E
  • Organizer: Animal Science Congress 2010 of the Asian-Australian Association of Animal Production Societies (AAAP)
  • Place of Presentation: National Pingtung University of Science and Technology (NPUST), Pingtung, Taiwan
  • Year and Date: 20100823-0827
• [Presentation] Possible implication of satellite cells in regenerative motoneuritogenesis: HGF and FGF2 upregulate neural chemorepellent Sema3A expression (2010)
  • Author(s): Tatsumi, R.,Do, M.-K. Q., Shimizu, N., Sankoda, Y., Anderson, J. E., Sato, Y., Suzuki, T., Mizunoya, W., Ikeuchi, Y., and Allen, R. E.
  • Organizer: the 6thWorld Congress on Biomechanics (WCB) 2010, in conjunction with the 14thInternational Conference on Biomedical Engineering (ICBME) 2010 and the 5thAsian Pacific Conference on Biomechanics (APBiomech) 2010
  • Place of Presentation: Suntec Convention and Exhibition Center, Singapore
  • Year and Date: 20100801-0806
• [Presentation] Mechano-biology on stretch-induced activation of resident myogenic stem cells. (2010)
  • Author(s): Tatsumi, R.,Anderson, J. E., and Allen, R. E
  • Organizer: the 6thWorld Congress on Biomechanics (WCB) 2010, in conjunction with the 14thInternational Conference on Biomedical Engineering (ICBME) 2010 and the 5thAsian Pacific Conference on Biomechanics (APBiomech) 2010
  • Place of Presentation: Suntec Convention and Exhibition Center, Singapore
  • Year and Date: 20100801-0806
• [Presentation] Possible implication of satellite cells in regenerative motoneuritogenesis: temporal coordination of HGF/FGF-2/TGF-beta may regulate neural chemorepellent Sema3A expression (2010)
  • Author(s): Do, M.-K. Q., Shimizu, N., Sato, Y., Suzuki, T., Tatsumi, R.,Mizunoya, W., Ikeuchi, Y., Anderson, J. E., and Allen, R. E
  • Organizer: 2010 FASEB Summer Research Conference on "Skeletal Muscle Satellite & Stem Cells"
  • Place of Presentation: Carefree, AZ, US
  • Year and Date: 20100718-0723
• [Presentation] 筋肥大・再生における衛星細胞の役割 (2010)
  • Author(s): 辰巳隆一
  • Organizer: 北大食肉科学セミナー「筋細胞の謎:筋(筋原線維)形成・再生・肥大の謎を解き明かす」
  • Place of Presentation: 北海道大学大学院農学研究院, 北海道札幌市
  • Year and Date: 2010-12-02
• [Remarks]
  • URL: http://hyoka.ofc.kyushu-u.ac.jp/search/details/K000315/research.html
• [Remarks]
  • URL: http://www.agr.kyushu-u.ac.jp/lab/muscle_and_meat/