2013 Fiscal Year Final Research Report
Establishment of reproductive technology by centri-fusion method for porcine oocytes
| Project/Area Number |
22380153
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
KIKUCHI Kazuhiro 独立行政法人農業生物資源研究所, 動物発生分化研究ユニット, 上級研究員 (20360456)
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| Co-Investigator(Kenkyū-buntansha) |
NAOMI Kashiwazaki 麻布大学, 獣医学部, 教授 (90298232)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 応用動物 / 発生・分化 / 発生工学 / 細胞操作 / 受精 |
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| Research Abstract |
The objective of the study is to establish reproductive biotechnologies without micromanipulation but by fusion of ooplasmic fragments obtained after centrifugations of porcine oocytes. The fragments were consisted of brownish and transparent ones; the former one contains a lot of mitochondria. The oocytes reconstructed from brownish fragments showed better fertilization ability. The oocytes showing low developmental ability were fused with the fragments, their fertilization and developmental abilities were improved, of which abilities were not different between two types of fragments. Furthermore, fusion of the fragments prepared from fertilized oocytes with the matured ones showed oocyte activation ability. These findings are now expected for further improvements of the technologies.
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