2012 Fiscal Year Final Research Report
Functional study on large glycans on the surface of developing embryos
| Project/Area Number |
22380187
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Nagoya University |
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Principal Investigator |
KITAJIMA Ken 名古屋大学, 生物機能開発利用研究センター, 教授 (80192558)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Chihiro 名古屋大学, 生物機能開発利用研究センター, 准教授 (10343211)
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| Co-Investigator(Renkei-kenkyūsha) |
HIBI Masahiko 名古屋大学, 生物機能開発利用研究センター, 教授 (40273627)
TANAKA Hiroshi 東京工業大学, 理工学研究科, 准教授 (40334544)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 糖鎖 / 発生・分化 / 生理活性 / タンパク質 / 再生医学 / 膜マイクロドメイン / 細胞接着 / 魚類 |
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| Research Abstract |
To address a question why the extremely large glycans are attached on the embryonic cell surface, we studied on two examples of glycoprotein that had been found in madaka fish by ourselves. First, the protein with LeX-containing large glycans, named LeX-gp, was successfully purified. The glycans were shown to contain a multiply tandem repeat of LeX-structure, and to be involed in cell adhesion. Second, the egg cortical vesicular glycoprotein that is secreted from egg at fertilization, named hyosophorin, was found to be involved in cortical reaction or egg activation and the progress of early development of medaka. We revealed that the peptide part is important for its cell proliferation stimulating activity through its binding to a specific receptor, while the glycan part is rather required for the cortical reaction than for the cell growth of embryonic cells. Taken together, we postulate a hypothesis that , once associated with the cell surface, the huge glycans drastically change the surface atmosphere to affect the physiology of cell significantly.
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