Molecular characterization of sensitized house dust mite allergens for the generation of tailor-made vaccine

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380190
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Hiroshima University
• Principal Investigator: ONO Kazuhisa 広島大学, 大学院・先端物質科学研究科, 教授 (10144883)
• Co-Investigator(Kenkyū-buntansha): AKI Tsunehiro 広島大学, 大学院・先端物質科学研究科, 准教授 (80284165) ; KAWAMOTO Seiji 広島大学, 大学院・先端物質科学研究科, 准教授 (90294537)
• Project Period (FY): 2010 – 2012
• Keywords: ダニアレルギー / 分子診断 / component-resolved diagnosis (CRD) / 感作アレルゲン

Research Abstract

Allergen-specific immunotherapy (SIT) is the only promising treatment of allergic disorders, but current SIT has problems such as the risk of side effects. Objective of this study is to construct a recombinant house dust mite allergen library, and to apply those allergen molecules for the development of molecular diagnostics and immunotherapeutics. Here, we showed a successful and comprehensive production of 23 major house dust mite allergens as a functional recombinant proteins. By using those recombinant allergen molecules, we also developed a molecular diagnostic system that enabled us to determine sensitized allergens among mite-allergic individuals.

Research Products

• [Journal Article] Utilization of waste syrup for production of polyunsaturated fatty acids and xanthophylls by Aurantiochytrium (2013)
  • Author(s): Iwasaka H., Aki T., Adachi H., Watanabe K., Kawamoto S., Ono K
  • Journal Title: J. Oleo Sci
  • Peer Reviewed
• [Journal Article] Competitive advantage and tolerance of the selected shochu yeast in barley shochu mash (2013)
  • Author(s): Takashita H., Fujihara E., Furutera M., Kajiwara Y., Shimoda M., Matsuoka M., Ogawa T., Kawamoto S., Ono K
  • Journal Title: J. Biosci.Bioeng
  • DOI: DOI:10.1016/j.jbiosc.2013.01.005
  • Peer Reviewed
• [Journal Article] Genetic instability of constitutive acid phosphatase in shochu and sake yeast (2013)
  • Author(s): Takashita H., Kajiwara Y., Shimoda M., Matsuoka M., Ogawa T., Ono K
  • Journal Title: J. Biosci. Bioeng
  • DOI: DOI:10.1016/j.jbiosc.2013.01.006
  • Peer Reviewed
• [Journal Article] Unexpected T cell regulatory activity of anti-histone H1 autoantibody: its mode of action in regulatory T cell-dependent and -independent manners (2013)
  • Author(s): Takaoka Y. , Kawamoto S., Katayama A., Nakano T., Yamanaka Y., Takahashi M., Shimada Y., Chiang K. C., Ohmori N., Aki T., Goto T., Sato S., Goto S., Chen C. L., Ono K
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 431 Pages: 246-252
  • Peer Reviewed
• [Journal Article] Immunological and regenerative aspects of hepatic mast cells in liver allograft rejection and tolerance (2012)
  • Author(s): Nakano T., Lai C. Y., Goto S., Hsu L. W., Kawamoto S., Ono K., Chen K. D., Lin C. C., Chiu K. W., Wang C. C., Cheng Y. F., Chen C. L
  • Journal Title: PLoS ONE Volume: 7 Pages: e37202
  • Peer Reviewed
• [Journal Article] Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen (2012)
  • Author(s): Ohkouchi K., Kawamoto S., Tatsugawa K., Takaoka Y., Yoshikawa N., Miyauchi S., Aki T., Yamashita M., Murooka Y., Ono K
  • Journal Title: J. Biosci. Bioeng Volume: 113 Pages: 536-541
  • Peer Reviewed
• [Journal Article] Sake lees fermented with lactic acid bacteria prevents allergic rhinitis-like symptoms and IgE-mediated basophil degranulation (2011)
  • Author(s): Kawamoto S., Kaneoke M., Ohkouchi K., Amano Y., Takaoka Y., Kume K., Aki T., Watanabe K., Kadowaki M., Hirata D., Ono K
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 75 Pages: 140-144
  • Peer Reviewed
• [Journal Article] Autoimmunity and liver transplantation immunology (2011)
  • Author(s): Goto S., Nakano T., Hsu L. W., Chiang K. C., Lai C. Y., Kawamoto S., Ono K., Chen K. D., Ohmori N., Goto T., Sato S., Wang C. C., Jawan B., Cheng F. Y., Chen C. L
  • Journal Title: Curr. Trends Immunol Volume: 12 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Metabolism and synthesis of lipids in the polyunsaturated fatty acid-producing fungus Mortierella alliacea (2011)
  • Author(s): Jermsuntiea W., Aki T., Kawamoto S., Ono K
  • Journal Title: J. Oleo Sci Volume: 60 Pages: 11-17
  • Peer Reviewed
• [Journal Article] Purification and characterization of intracellular lipase from the polyunsaturated fatty acid-producing fungus Mortierella alliacea (2011)
  • Author(s): Jermsuntiea W., Aki T., Toyoura R., Iwashita K., Kawamoto S., Ono K
  • Journal Title: N. Biotechnol Volume: 28 Pages: 158-164
  • Peer Reviewed
• [Journal Article] Molecular cloning and immunochemical characterization of a new Japanese cedar pollen allergen homologous to plant subtilisin-like serine protease (2010)
  • Author(s): Ibrahim A. R. N., Kawamoto S., Mizuno K., Shimada Y., Rikimaru S., Onishi N., Hashimoto K., Aki T., Hayashi T., Ono K
  • Journal Title: World Allergy Organ. J Volume: 3 Pages: 262-265
  • Peer Reviewed
• [Journal Article] Molecular cloning and immunochemical characterization of a novel major Japanese cedar pollen allergen belonging to the aspartic protease family (2010)
  • Author(s): Ibrahim A. R. N., Kawamoto S., Aki T., Shimada Y., Rikimaru S., Onishi N., Babiker E. E., Oiso I., Hashimoto K., Hayashi T., Ono K
  • Journal Title: Int. Arch. Allergy Immunol Volume: 152 Pages: 207-218
  • Peer Reviewed
• [Journal Article] A new lipid transfer protein homolog identified as an IgE-binding antigen from Japanese cedar pollen (2010)
  • Author(s): Ibrahim A. R. N., Kawamoto S., Nishimura M., Pak. S., Aki T., Diaz-Perales A., Salcedo G., Asturias J. A., Hayashi T., Ono K
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 74 Pages: 504-509
  • Peer Reviewed
• [Journal Article] Immunological aspects and therapeutic significance of an auto-Ab against histone H1 in a rat model of Con A-induced hepatitis (2010)
  • Author(s): Nakano T., Goto S., Lai C. Y., Hsu L. W., Takaoka Y., Kawamoto S., Chiang K. C., Shimada Y., Ohmori N., Goto T., Sato S., Ono K., Cheng Y. F., Chen C. L
  • Journal Title: Immunology Volume: 129 Pages: 547-555
  • Peer Reviewed
• [Journal Article] Selective detection of antigen-antibody reaction using Si ring optical resonators (2010)
  • Author(s): Fukuyama M., Yamatogi S., Ding H., Nishida M., Kawamoto C., Amemiya Y., Ikeda T., Noda T., Kawamoto S., Ono K., Kuroda A., Yokoyama S
  • Journal Title: Jpn. J. Appl. Phys Volume: 49 Pages: 04DL09-1-04DL09-4
  • Peer Reviewed
• [Journal Article] Effects of a fermented barley extract on subjects with slightly high serum uric acid or mild hyperuricemia (2010)
  • Author(s): Hokazono H., Omori T., Yamamoto T., Akaoka I., Ono K
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 74 Pages: 828-834
  • Peer Reviewed
• [Journal Article] Effects of single and combined administration of fermented barley extract and gamma-aminobutyric acid on the development of atopic dermatitis in NC/Nga mice (2010)
  • Author(s): Hokazono H., Omori T., Ono K
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 74 Pages: 135-139
  • Peer Reviewed
• [Journal Article] Hydrolyzed Konjac glucomannan suppresses IgE production in mice B cells (2010)
  • Author(s): Suzuki H., Oomizu S., Yanase Y., Onishi N., Uchida K., Mihara S., Ono K., Kameyoshi Y., Hide M
  • Journal Title: Int. Arch. Allergy Immunol Volume: 152 Pages: 122-130
  • Peer Reviewed
• [Presentation] Component-resolved molecular diagnosis of house dust mite allergy using a comprehensive repertoire of recombinant allergens (2013)
  • Author(s): Kishikawa Y., Kawamoto S., Nakahara H., Watakabe Y., Ota Y., Aki T., Asaoku Y., Hayashi T., Tanaka A., Ono K
  • Organizer: The EAACI-WAO Congress 2013
  • Place of Presentation: Milano, Italy (accepted for oral presentation)
  • Year and Date: 20130622-26
• [Presentation] ダニアレルギーのcomponent-resolved diagnosis 実現に向けた基盤技術の開発 (第2 報) (2012)
  • Author(s): 中原輝、河本正次、渡壁徳治、岸川由季、秋庸裕、麻奥良子、林鷹治、田中明彦、小埜和久
  • Organizer: 第62回日本アレルギー学会秋季学術大会
  • Place of Presentation: 大阪
  • Year and Date: 20121129-1201
• [Presentation] Comprehensive expression of recombinant house dust mite allergens for component-resolved diagnosis (2011)
  • Author(s): Watakabe Y., Kawamoto S., Nakahara H., Hiragun T., Aki T., Asaoku Y., Hayashi T., Mihara S., Hide M., Ono K
  • Organizer: The 22thWorld Allergy Congress (WAC 2011)
  • Place of Presentation: Cancun, Mexico
  • Year and Date: 20111204-08
• [Presentation] ダニアレルギーの component-resolved diagnosis 実現に向けた基盤技術の開発 (2011)
  • Author(s): 中原輝、河本正次、渡壁徳治、秋庸裕、麻奥良子、林鷹治、小埜和久
  • Organizer: 第61回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
  • Year and Date: 20111110-12
• [Presentation] Recombinant allergens for immunotherapy (2010)
  • Author(s): Kawamoto S., Aki T,, Ono K
  • Organizer: BIT's 1st Annual World Congress of Immunodiseases and Therapy 2010
  • Place of Presentation: Beijing, China (invited lecture)
  • Year and Date: 20100515-17