2012 Fiscal Year Final Research Report
Development of Capture and CollectionMethods of Circulating Tumor Cells Utilizing Photocleavable Linkers
| Project/Area Number |
22390005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical pharmacy
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
AOKI Shin 東京理科大学, 薬学部生命創薬科学科, 教授 (00222472)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Ryo 東京理科大学, 生命医科学研究所, 教授 (20159453)
HAYASE Masanori 東京理科大学, 理工学部, 准教授 (70293058)
NAKATSURA Tetsuya 国立がん研究センター東病院, 臨床開発センター, 機能再生室長 (30343354)
ITOH Masaaki 国立がん研究センター東病院, 臨床開発センター, 消化器科医長 (40312144)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 血中循環腫瘍細胞 / 光分解反応 / がん診断 / シリコン基板 / 抗体 |
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| Research Abstract |
Circulating tumor cells (CTCs) have been defined as cancer cells of solid tumor origin found in the peripheral blood. It isgenerally considered that these CTCs detach from primary tumors of patients, go into the bloodstream, travel around in the body, and then attach to the remote tissues. Accordingly, it is generally described that the quantitative detection and analysis of CTC is considered to be useful for the personal diagnosis of cancer patients. In this work, a device for the capture and recollection of live target cells is developed The platform was a silicon (Si) wafer modified with an anti-HEL antibody (anti-HEL-IgG, HEL= hen egg lysozyme) through a photocleavable 3-amino-3-(2-nitrophenyl)propionic acid (ANP) linker. The modification processes of the Si wafer surface were monitored by Fourier transform infrared spectroscopy by attenuated total reflection (FTIR-ATR) and fast-scanning atomic force microscopy (FS-AFM). The attachment of IgG and its release reaction on the Si surface via the photochemical cleavage of the ANP linker were observed by FS-AFM and an enzyme-linked immunosorbent assay (ELISA). Furthermore, it was possible to selectively collect SP2/O cells that express HEL on their cell membranes (SP2/O-HEL) on the Si wafer device. Photochemical cleavage of the ANP linker facilitated the effective release of living SP2/O cells, whose viability was verified by staining experiments using tripan-blue. Moreover, it was possible to re-culture the recovered cells. This methodology represents an effective strategy for isolating intact target cells in the biological and medicinal sciences and related fields.
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Research Products
(46 results)
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[Journal Article] Design and Synthesis of A Fluorescent Probe for Zn2+, 8-Benzenesulfonyloxy-5,7-Bis(N,N-dimethylaminosulfonyl)quinoline-Pendant 1,4,7,10-Tetraazacyclododecane and Its Caged Derivative that Activated by Complexation with Zn2+2010
Author(s)
Ryosuke Ohshima, Masanori Kitamura, Akinori Morita, Motoo Shiro, Eiichi Kimura, Masahiko Ikekita, and Shin Aoki
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Journal Title
Inorganic Chemistry
Volume: 49 (3)
Pages: 888-899
Peer Reviewed
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[Presentation] The Selective Capture and Collection of Live Target Cells using a Photoreactive Silicon WaferDevice Modified with Antibodies via Photocleavable Linkers2012
Author(s)
Ariyasu, S.;Hanaya, K.; Watanabe, E.; Hoshi, M.; Suzuki, T.; Horie, K.Hayase, M.; Abe, R.; Aoki, S.
Organizer
3th Tetrahedron Symposium- Asia Edition-
Place of Presentation
Taipei, Taiwan
Year and Date
20121127-30
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[Presentation] Aoki, S., Ariyasu, S.;Watanabe, E.; Hoshi, M.; Suzuki, T.; Horie, K.; Hayase, M.; Abe, R.2012
Author(s)
Development of a Photoreactive Silicon Device Modified with Antibodies via Photocleavable Linkers for the Selective Capture and Collection of Live Target Cells
Organizer
17th Malaysian Chemical Congress (17MCC)
Place of Presentation
Kuala Lumpur, Malaysia (Invited lecture)
Year and Date
20121015-17
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[Presentation] Development of Photochemical Devices for Diagnosis and Treatment of Cancer2012
Author(s)
Aoki, S.; Ariyasu, S.; Hisamatsu, Y.; Moromizato, S.; Shibuya, A.; Watanabe, E.; Suzuki, T.; Hayase, M.; and Abe, R.
Organizer
1st International Postgraduate Conference on Pharmaceutical Sciences
Place of Presentation
Puncak Alam, Malaysia (Plenary Lecture)
Year and Date
20120628-30
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[Presentation] Aoki, S.; Hanaya, K.; Kobayashi, K.;Mizuseda, Y.; Saijo, S.; Ariyasu, S.; Yamaguchi, Y.; Kurosaki, M.; Yamato, I.2012
Author(s)
Design and Synthesis of 8-Quinolinol-Based Inhibitors of Dinuclear Zn2+Hydrolases: Interplay of Zn2+Fluorophores and Zn2+Enzyme Inhibitors
Organizer
BIT's 3rd Annual International Congress of Medichem-2012 (ICM-2012)
Place of Presentation
Beijing, China (Invited lecture)
Year and Date
20120512-14
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