2013 Fiscal Year Final Research Report
Analytical methods for protein kinase profiling by using fluorescent Phos-tag molecules
| Project/Area Number |
22390006
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Hiroshima University |
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Principal Investigator |
KOIKE Tohru 広島大学, 医歯薬保健学研究院, 教授 (90186586)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | プロテオミクス / キナーゼ / フォスファターゼ / 蛍光分析 / ケミカルバイオロジー |
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| Research Abstract |
By utilizing the Phos-tag molecule and its fluorescent derivatives, we have developed convenient and reliable methods for the analysis of phosphorylated biomolecules such as peptides and proteins. In this program, we applied the Phos-tag technology to (1) FRET system for kinase/phosphatase profiling using Phos-tag, (2) fluorometric analysis of phosphorylated proteins, (3) analysis of phosphorylation status of multi-phosphorylated biomolecules. We believe that our Phos-tag technology will result in great progress in phosphoproteomics.
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Research Products
(21 results)
