2012 Fiscal Year Final Research Report
Cellular and molecular study on live imaging of expression of sperm functional proteins and its relation to infertility
| Project/Area Number |
22390033
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Chizuru 千葉大学, 大学院・医学研究院, 講師 (80347054)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEKAWA Mamiko 千葉大学, 大学院・医学研究院, 助教 (20181571)
YAMATOYA Kenji 千葉大学, 大学院・医学研究院, 特任研究員 (80447309)
KAMIMURA Kyoko 千葉大学, 大学院・医学研究院, 技術専門職員 (20422264)
MUTOH Tohru 千葉大学, 大学院・医学研究院, 技術専門職員 (30422265)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 精子 / 受精 / エクアトリン / ライブセルイメージング / 遺伝子改変マウス |
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| Research Abstract |
We have established Eqtn-EGFP-TG mouse and ODF2-EGFP-TG mouse lines, and tried to take live-image of the molecular change from the acrosome reaction throughout to egg activation. A whole cell imaging (80-100nm acrosomal membrane complex) of Eqtn-EGFP-TG sperm was obtained by new microscopy STED system without sectioning, which suggested a possibility of live cell imaging during fertilization. Galnt3 transferase is required for O-glycosylation of carbohydrate chain branching from threonine 138 of equatorin molecule. We clarified the distribution abnormality of egg activation related MN13 molecule in the infertile patient who have round-head sperm (globozoospermia), by comparing the expression with that of the model mouse GOPC-/- spermatozoa. Based on these analyses, we proposed the mechanism leading to egg activation failure in human sperm.
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[Journal Article] Tysndl deficiency in mice interferes with the peroxisomal location of PTS2 enzymes2013
Author(s)
Mizuno Y, Ninomiya Y, Nakachi Y, Iseki M, Iwasa H, Akita M, Tsukui T, ShimozawaN, Ito C, Toshimori K, Nishimura M, Hara H, Maeba R, Okazaki T, Alodaib AN, Amoudi MA,Jacob M, Alkuraya FS, Horai Y, Watanabe M, Motegi H, Wakana S, Noda T, Kurochkin IV, Mizuno Y, Schonbach C, Okazaki Y
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Journal Title
causinglipid metabolic abnormalities and maleinfertility. PLoS genet
Volume: 9(2)
Pages: e1003286
Peer Reviewed
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[Journal Article] Galnt3 deficiency disrupts acrosome formation and leads to oligoasthenoteratozoospermia2013
Author(s)
Miyazaki T, Mori M, Yoshida CA, Ito C,Yamatoya K, Moriishi T, Kawai Y, Komori H, Kawane T, Izumi S, Toshimori K, Komori T
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Journal Title
Histochem and Cell Biol
Volume: 139(2)
Pages: 339-354
DOI
Peer Reviewed
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[Journal Article] Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage2011
Author(s)
Sato B, Katagiri YU, Miyado K, Okino N, Ito M, Akutsu H, Okita H, Umezawa A, Fujimoto J, Toshimori K, Kiyokawa N
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Journal Title
BMC Dev Biol
Volume: 11
Pages: 22
DOI
Peer Reviewed
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[Journal Article] GalNAcβ1,3-linked paragloboside carries the epitope of a sperm maturation-related glycoprotein that is recognized by the monoclonal antibody MC1212011
Author(s)
Katagiri YU, Sato B, Yamatoya K, Taki T, Goto-Inoue N, Setou M, Okita H, Fujimoto J, Ito C, Toshimori K, Kiyokawa N
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Journal Title
Biochem Biophys Res Commun
Volume: 406(3)
Pages: 326-331
DOI
Peer Reviewed
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[Presentation] Oocyte-activation ability correlates with presence of perinuclear theca substance MN13,whose organization is associated with acrosomeformation2010
Author(s)
Ito C, Yamatoya K, Yoshida K, Akutsu H, Yao R, Kyono K, Suzuki-Toyota F, Toyama Y, Maekawa M, Noda T, Toshimori K
Organizer
11th International Symposium on Spermatology Okinawa 2010 (Poster)
Place of Presentation
Okinawa convention center, Okinawa (Japan)
Year and Date
2010-06-25
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[Presentation] Further characterization of acrosomal protein Equatorin2010
Author(s)
Yamatoya K, Yoshida K, Ito C, Maekawa M, Yanagida M, Araki Y, Miyado K, Toyama Y, Suzuki-Toyoda F, Toshimori K
Organizer
11th International Symposium on Spermatology Okinawa 2010 (Poster)
Place of Presentation
Okinawa convention center, Okinawa (Japan)
Year and Date
2010-06-25
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[Remarks] (1)新しい検査法に基づく精子の機能診断法、選別保存法の開発と生殖医療応用2012年4月11日産経新聞(2)報道関連情報
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[Remarks] (2)新しい精子の機能診断法、選別保存法の開発と生殖医療応用千葉日報2012年4月11日(2)報道関連情報
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[Remarks] (1)年森清隆なのはなコンペ2012ベンチャー志向先端研究部門なのはな賞(教員版)受賞2012年4月9日 (3)受賞歴
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[Remarks] (2)年森清隆なのはなコンペ2012ベンチャー志向先端研究部門双葉電子記念財団助成金ちばぎんひまわり賞受賞2012年4月9日 (3)受賞歴
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