Novel functional linkages of drinking・feeding behavior and TRP channels: Clarification by the post-genome approach

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390044
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: UETA Yoichi 産業医科大学, 医学部, 教授 (10232745)
• Co-Investigator(Kenkyū-buntansha): FUJIHARA Hiroaki 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助教 (10369051) ; ISHIKURA Toru 産業医科大学, 医学部, 助教 (70580281)
• Project Period (FY): 2010 – 2012
• Keywords: 飲水行動 / 摂食行動 / TRPチャネル / ペプチド / 遺伝子改変動物 / Fosタンパク / 浸透圧感受性

Research Abstract

The purpose of the study was to investigate the roleof TRP channels known as osmotic sensors, using TRPV1 and TRPV4 knockout mice in comparison with wild type concerning about the feeding and drinking behavior. We revealed that TRPV1 and TRPV4 channels concerned drinking behavior and water and food intake after water deprivation.

Research Products

• [Journal Article] A c-fos-monomeric red fluorescent protein l fusion transgene is differentially expressed in rat forebrain and brainstem after chronic dehydration and rehydration (2013)
  • Author(s): Yoshimura, M. Ohkubo, J. Katoh, A. Ohno, M. Ishikura, T. Kakuma, T. Yoshimatsu, H. Murphy, D. Ueta, Y
  • Journal Title: Journal of Neuroendocrinology Volume: 25(5) Pages: 478-487
  • DOI: DOI:10.1111/jne.12022
  • Peer Reviewed
• [Journal Article] Induction of arginine vasopressin-enhanced green fluorescent protein expression in the locus coeruleus following kainic acid-induced seizures in rats (2012)
  • Author(s): Ohno, M. Fujihara, H. Iwanaga, M. Todoroki, M. Katoh, A. Ohbuchi, T. Ishikura, T. Hamamura, A. Hachisuka, K. & Ueta, Y
  • Journal Title: Stress Volume: 15(4) Pages: 435-442
  • DOI: DOI:10.3109/10253890.2011.637185
  • Peer Reviewed
• [Journal Article] In vitro morphological bud formation in organ-like three-dimensional structure from mouse ES cells induced by FGF10 signaling (2012)
  • Author(s): Ohbuchi, T. Takaki, M. Misawa, H. Suzuki, H. & Ueta, Y
  • Journal Title: Communicative & Integrative Biology Volume: 5(4) Pages: 1-4
  • DOI: DOI:10.4161/cib.20093
  • Peer Reviewed
• [Journal Article] Expression of the c-fos-monomeric red fluorescent protein l fusion gene in the spinal cord and the hypothalamic paraventricular nucleus in transgenic rats after formalin injection (2012)
  • Author(s): Ishikura, T. Suzuki, H. Yoshimura, M. Ohkubo, J. Katoh, A. Ohbuchi, T. Ohno, M.Fujihara, H. Kawasaki, M. Ohnishi,H. Nakamura, T. & Ueta, Y
  • Journal Title: Brain Research Volume: 1479 Pages: 52-61
  • DOI: DOI:10.1016/j. brainres.2012.08.033
  • Peer Reviewed
• [Journal Article] Pathophysiological function of oxytocin secreted by neuropeptides: A mini review (2012)
  • Author(s): Hashimoto, H. Uezono, Y. & Ueta, Y
  • Journal Title: Pathophysiology Volume: 19 Pages: 283-298
  • DOI: DOI:10.1016/j. pathophys.2012.07.005
  • Peer Reviewed
• [Journal Article] Molecular characterization and biological function of neuroendocrine regulatory peptide-3 in the rat (2012)
  • Author(s): Fujihara, H. Sasaki, K. Mishiro-Sato, E.Ohbuchi, T. Dayanithi, G. Yamasaki, M. Ueta, Y. & Minamino, N
  • Journal Title: Endocrinology Volume: 153(3) Pages: 1377-1386
  • DOI: DOI:10.1210/en.2011-1539
  • Peer Reviewed
• [Journal Article] Central diabetes insipidus associated with impaired renal aquaporin-1 expression in Inice lacking liver X receptorβ (2012)
  • Author(s): Gabbi, C. Kong, X. Suzuki, H. Kim, HJ. Gao, M. Jia, X. Ohnishi, H. Ueta, Y. Warner, M. Guan, Y. & Gustafsson, JA
  • Journal Title: Proceedings of the National Academy of Sciences, USA Volume: 109(8) Pages: 3030-3034
  • DOI: DOI:10.1073/pnas.1200588109
  • Peer Reviewed
• [Journal Article] Water deprivation induces appetite and alters metabolic strategy in Notomys alexis: unique mechanisms for water production in the desert (2012)
  • Author(s): Takei, Y. Bartolo, R. Fujihara, H. Ueta, Y. & Donald, J
  • Journal Title: Proceedings of the Royal Society Volume: B24(4) Pages: 539-553
  • DOI: DOI:10.1098/rspb.2011.2627
  • Peer Reviewed
• [Journal Article] Allylisothiocyanates and cinnamaldehyde potentlate mlnlature excltatory postsynaptic inputs in the sipraoptic nucleus in rats (2011)
  • Author(s): Yokoyama, T. Ohbuchi, T. Saito, T. Fujihara, H. Minami, K. Nagatomo, T. Uezono, Y. & Ueta, Y
  • Journal Title: European Journal of Pharmacology Volume: 655(1-3) Pages: 31-37
  • DOI: DOI:10.1016/j. ejphar.2011.01.011
  • Peer Reviewed
• [Journal Article] Central effects of ghrelin, a unique peptide, on apPetite and fluid/water drinking behavior (2011)
  • Author(s): Hashimoto, H. & Ueta, Y
  • Journal Title: Current Protein and Peptide Science Volume: 12(4) Pages: 280-287
  • Peer Reviewed
• [Journal Article] Angiotensin II type 1 receptor signaling regulates feeding behavior through anorexigenic corticotropin-releasing hormone in hypothalamus (2011)
  • Author(s): Yamamoto, R. Akazawa, H. Fujihara, H. Ozasa, Y. Yasuda, N. Ito, K. Kudo, Y. Qin, Y. Ueta, Y. & Komuro, I
  • Journal Title: Journal of Biological Chemistry Volume: 286(24) Pages: 21458-21465
  • DOI: DOI:10.1074/jbc. M110.192260
  • Peer Reviewed
• [Journal Article] Induction of Fos expression in the rat forebrain after intragastric administration of monosodium L-glutamate, glucose and NaCl (2011)
  • Author(s): Otsubo, H. Kondoh, T. Shibata, M. Ueta, Y. &Torii, K
  • Journal Title: Neuroscience Volume: 196 Pages: 97-103
  • DOI: DOI:10.1016/j. neuroscience.2011.09.003
  • Peer Reviewed
• [Journal Article] Highly visible expression of an oxytocin-monomeric red fluorescent protein 1 fusion gene in the hypothalamus and posterior pituitary of transgenic rats (2011)
  • Author(s): Katoh, A. Eujihara, H. Ohbuchi, T. Onaka, T. ashimoto, T. Kawata, M. Suzuki, H. & Ueta, Y
  • Journal Title: Endocrinology, (Cover article of the issue) Volume: 152(7) Pages: 2768-2774
  • DOI: DOI:10.1210/en.2011-0006
  • Peer Reviewed
• [Journal Article] Hypothalamic vasopressin response to stress and various physiological stimuli: Visualization in transgenic animal models (2011)
  • Author(s): Ueta, Y. Dayanithi, G.&Fujihara, H
  • Journal Title: Hormones and Behavior Volume: 59(2) Pages: 221-226
  • DOI: DOI:10.1016/j. yhbeh.2010.12.007
  • Peer Reviewed
• [Journal Article] Diurnal changes of arginine vasopressin-enhanced green fluorescent protein fusion transgene expression in the rat suprachiasmatic nucleus (2010)
  • Author(s): Maruyama, T. Ohbuchi, T. Eujihara, H. Shibata, M. Mori, K. Murphy, D. Dayanithi, G.& Ueta, Y
  • Journal Title: Peptides Volume: 31(11) Pages: 2089-2093
  • DOI: DOI:10.1016/j.peptides.2010.08.010.
  • Peer Reviewed
• [Presentation] 急性痺痛ストレスに対する内分泌反応および行動変化へのTRPV1およびTRPV4の役割～ノックアウトマウスを用いた検討～ (2013)
  • Author(s): 石倉 透、鈴木 仁士、松浦 孝紀、大久保 淳一、吉村 充弘、丸山 崇、大西 英生、中村 利孝、上田 陽一
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: タワーホール船堀(東京都)
  • Year and Date: 20130327-29
• [Presentation] Neuroendocrine response and nocifensive behavior after nociceptive stimulation in TRPV1 and TRPV4 knockout mice (2012)
  • Author(s): Ishikura, T. Suzuki, H. Matsuura, T. Yoshimura, M. Ohkubo, J. Maruyama, T. Ohnishi, H .& Ueta, Y
  • Organizer: 15th International Congress on Hormonal Steroids and Horlnones & Cancer
  • Place of Presentation: Ishikawa Ongakudo (Kanazawa, Japan)
  • Year and Date: 20121115-17
• [Presentation] Fluorescent visualization of vasopressin and oxytocin neurons in the hypothalamus and their terminals in the hypothalalnus in transgenic rats (2012)
  • Author(s): Ueta, Y
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: Kyoto International Conference Center (Kyoto, Japan)
  • Year and Date: 20120826-29
• [Presentation] 急性疹痛ストレスに対するTRPV1およびV4の役割-ノックアウトマウスを用いた検討- (2012)
  • Author(s): 石倉 透、鈴木 仁士、松浦 孝紀、大久保 淳一、吉村 充弘、大野 素子、丸山 崇、大西 英生、上田 陽一
  • Organizer: 第22回日本病態生理学会
  • Place of Presentation: 湯布院厚生年金病院(大分県)
  • Year and Date: 20120804-05
• [Presentation] Efects of nociceptive stimulation on fos expression and behavior in mice (2012)
  • Author(s): Ueta, Y. Ishikura, T. Matsuura, T. Yoshimura, M. Ohkubo, J. Ohnishi, H
  • Organizer: 21^<st> International Behavioral Neuroscience Society annual meeting
  • Place of Presentation: Kailua-Kona (Hawaii, USA)
  • Year and Date: 20120605-10
• [Presentation] Neuroendocrine regulatory peptide-3のラット視床下部における分布と生理機能 (2012)
  • Author(s): 藤原 広明、三城一佐藤 恵美、佐々木一樹、大淵 豊明、山崎 基生、上田 陽一
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館(長野県)
  • Year and Date: 20120329-31
• [Presentation] 体液調節研究のための新しい遺伝子的アプローチ (2012)
  • Author(s): 上田陽一
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館(長野県)
  • Year and Date: 20120329-31
• [Presentation] Drinking and feeding behavior based on peptides and TRP channels (2012)
  • Author(s): Ueta, Y
  • Organizer: The 3^<rd> International Symposium of KoSCI & the 2^<nd> AISCRIB
  • Place of Presentation: Hoam Faculty House, Seoul National University (Seoul, Korea)
  • Year and Date: 2012-11-01
• [Presentation] 急性痺痛ストレスに対するTRPV1およびTRPV4の役割～ノックアウトマウスを用いた検討～ (2012)
  • Author(s): 石倉 透、鈴木 仁士、松浦 孝紀、大久保 淳一、吉村 充弘、大野 素子、 丸山 崇、上田 陽一、大西 英生、中村利孝
  • Organizer: 第30回産業医科大学学会
  • Place of Presentation: 産業医科大学(福岡県)
  • Year and Date: 2012-10-20
• [Presentation] Effects of kainic acid on arginine vasopressin (AVP) synthesis in vivo and an isolated AVP neuron in vitro (2012)
  • Author(s): Ueta, Y. Iwanaga, M. Ohkubo, J
  • Organizer: Neurophysiology of Neurosecretory Neurons Workshop
  • Place of Presentation: Tuklane University (New Orleans, USA)
  • Year and Date: 2012-10-13
• [Presentation] Expression of Fos protein in the spinal cord and hypothalalnus after acute arthritis in the o-fos-monomeric red fluorescent protein transgenic rats (2011)
  • Author(s): Ishikura, T. Suzuki, H. Ohnishi, H. Nakamura, T & Ueta, Y
  • Organizer: SICOT 2011
  • Place of Presentation: Prague Congress Center (Prague , Czech Republic)
  • Year and Date: 20110906-09
• [Presentation] Neuroendocrine regulatory peptide-3: anatomical distributions in the hypothalamus and physiological role on the hypothalamo-neurohypophysial system in rats (2011)
  • Author(s): Ueta, Y. Fujihara, H. Mishiro-Sato, E. Sasaki, K. Ohbuchi, T. Dayanithi, G. Yamasaki, M.&Minamino, N
  • Organizer: 9th World Congress on Neurohypophysial Hormones
  • Place of Presentation: Noutherstern University (Boston, USA)
  • Year and Date: 20110727-30
• [Presentation] 急性疹痛ストレスに対するTRPV1およびTRPV4の役割～ノックアウトマウスを用いた検討～ (2011)
  • Author(s): 石倉 透、有冨 貴史、鈴木 仁士、大久保 淳一、吉村 充弘、大野 素子、加藤明子、上田陽一
  • Organizer: 第39回自律神経生理研究会
  • Place of Presentation: 日本光電本社研究センター(東京都)
  • Year and Date: 2011-12-03
• [Presentation] Diurnal changes of arginine vasopressin-enhanced green fluorescent protein fusion transgene expression in the rat suprachiasmatic nucleus (2010)
  • Author(s): Maruyama, T. Ohbuchi, T. Fujihara, H. Shibata, M. Murphy, D. Dayanithi, G.& Ueta, Y
  • Organizer: 5th International Peptide Symposium
  • Place of Presentation: Kyoto International Conference Center (Kyoto, Japan)
  • Year and Date: 20101204-09
• [Presentation] バゾプレッシン産生ニューロンにおけるプリン作動性受容体とバネキシンチャネル: 電気生理学的検討 (2010)
  • Author(s): 大淵 豊明、横山 徹、齋藤 健、藤原広明、上田陽一
  • Organizer: 第37回日本神経内分泌学会学術集会
  • Place of Presentation: 京都大学芝蘭会館(京都府)
  • Year and Date: 20101015-16
• [Presentation] オキシトシン-mRFPトランスジェニックラットにおける赤色蛍光の観察:浸透圧負荷による検討 (2010)
  • Author(s): 加藤明子、藤原 広明、石倉 透、大淵 豊明、鈴木 秀明、上田 陽一
  • Organizer: 第37回日本神経内分泌学会学術集会
  • Place of Presentation: 京都大学芝蘭会館(京都府)
  • Year and Date: 20101015-16
• [Presentation] Regulation of stress responses by peptides:New transgenic animal models (2010)
  • Author(s): Ueta, Y
  • Organizer: The 7^<th> International Congress and Neuroendocrinology
  • Place of Presentation: Rouen, France
  • Year and Date: 20100711-15
• [Presentation] ラットバゾプレッシン産生ニューロンにおけるBKチャネルについて (2010)
  • Author(s): 大淵 豊明、横山 徹、藤原 広明、鈴木秀明、上田陽一
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手県)
  • Year and Date: 20100519-21
• [Presentation] 食 思不振一悪液質動物モデルにおける視床下部神経ペプチドの反応: (2010)
  • Author(s): 上田陽一
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール(岩手県)
  • Year and Date: 20100519-21