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2012 Fiscal Year Final Research Report

Transcriptional mechanism of noncoding RNA

Research Project

  • PDF
Project/Area Number 22390057
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionSaitama Medical University

Principal Investigator

KUROKAWA Riki  埼玉医科大学, 医学部, 教授 (70170107)

Co-Investigator(Renkei-kenkyūsha) MATSUSITA Akio  埼玉医科大学, 医学部, 客員講師 (50402269)
OGAWA Sumito  東京大学, 医学部附属病院, 講師 (20323579)
Project Period (FY) 2010 – 2012
Keywords酵素 / 遺伝子 / 核酸 / 生体分子 / 発現制御
Research Abstract

We have identified that promoter-derived noncoding RNA (pncRNA) is transcribed from the promoter region of the cyclin D1 gene and represses the expression of the gene (Nature 434: 126, 2008). We have also determined a transcriptional initiation site of pncRNA and purified transcription factors inducing the transcription of pncRNA. The purification provided five proteins involving DNA repair and two of paraspeckle-related proteins. These data suggest that the transcription of the pncRNA requires the DNA repair system and the transcription is located in the paraspeckle area of nuclei. This will enhance elucidation of novel mechanisms of the ncRNA transcription.

  • Research Products

    (21 results)

All 2013 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (10 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Structure of noncoding RNA is a determinant of function of RNA binding proteins in transcriptional regulation.2012

    • Author(s)
      Oyoshi, T., and Kurokawa, R.
    • Journal Title

      Cell & Bioscience

      Volume: 2 Pages: 1

    • DOI

      DOI:10.1186/2045-3701-2-1

    • Peer Reviewed
  • [Journal Article] Loop Lengths of G-Quadruplex Structures Affect the G-Quadruplex DNA Binding Selectivity of the RGG Motif in Ewing's Sarcoma.2011

    • Author(s)
      Takahama, K., Sugimoto, C., Arai, S., Kurokawa, R., and Oyoshi, T.
    • Journal Title

      Biochemistry

      Volume: 50 Pages: 5369-5378

    • DOI

      DOI:10.1021/bi2003857

    • Peer Reviewed
  • [Journal Article] Identification of Ewing's sarcoma protein as a G-quadruplex DNA- and RNA-binding protein2011

    • Author(s)
      Takahama, K., Kino, K., Arai, S., Kurokawa, R., and Oyoshi, T.
    • Journal Title

      FEBS Journal

      Volume: 278 Pages: 988-998

    • DOI

      DOI:10.1111/j.1742-4658.2011.08020.x

    • Peer Reviewed
  • [Journal Article] TLS and PRMT1 synergistically coactivate transcription at the survivin promoter through TLS arginine methylation2011

    • Author(s)
      Du, K., Arai, S., Kawamura, T., Matsushita, A., and Kurokawa, R.
    • Journal Title

      BBRC

      Volume: 404 Pages: 991-996

    • DOI

      DOI:10.1016/j.bbrc.2010.12.097

    • Peer Reviewed
  • [Journal Article] Long Noncoding RNA as a Regulator for Transcription. In: Ugarkovic, D.(ed) Long Non-Coding RNAs2011

    • Author(s)
      Kurokawa, R.
    • Journal Title

      Progress in Molecular and Subcellular Biology 51

      Volume: 2 Pages: 29-41

    • DOI

      DOI10.1007/978-3-642-16502-3_2

    • Peer Reviewed
  • [Journal Article] Id4, a new candidate gene for senile osteoporosis, acts as a molecular switch promoting osteoblast differentiation.2010

    • Author(s)
      okuzawa, Y., Yagi, K., Yamashita ,Y., Nakachi, Y., Nikaido, I., Bono, H., Ninomiya, Y., Kanesaki-Yatsuka, Y., Akita, M., Motegi, H., Wakana, S., Noda, T., Sablitzky, F., Arai, S., Kurokawa, R., Fukuda, T., Katagiri, T., Schonbach, C., Suda, T., Mizuno, Y., and Okazaki, Y.
    • Journal Title

      PLoS Genet

      Volume: 6 Pages: e1001019

    • Peer Reviewed
  • [Presentation] 長鎖非コード RNA 機能の分子機構-核酸医薬の可能性2012

    • Author(s)
      黒川 理樹
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2012-12-12
  • [Presentation] Long noncoding RNA as an epigenetic regulatory factor.2012

    • Author(s)
      Kurokawa, R.
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      北海道札幌市
    • Year and Date
      2012-09-20
  • [Presentation] Long noncoding RNA, a counterpart of microRNA, works as a transcriptional regulator. Is lncRNA a potential target for nucleic acid medicine?2012

    • Author(s)
      Kurokawa, R.
    • Organizer
      第4回日本 RNAi 研究会
    • Place of Presentation
      広島県広島市
    • Year and Date
      2012-08-30
  • [Presentation] Promoter-associated noncoding RNA and Arginine-specific Methylation of RNA-binding protein TLS.2012

    • Author(s)
      Kurokawa, R.
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Utah, USA
    • Year and Date
      2012-04-04
  • [Presentation] 長鎖非コード RNA とエピゲノム制御2011

    • Author(s)
      黒川 理樹
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2011-12-16
  • [Presentation] TLS-CHOP, an Oncogenic Fusion Product in Myxoid Liposarcoma, Attenuates Cell Adhesion Ability through Down-Regulation of Integrin α5β1.2011

    • Author(s)
      Kurokawa, R.
    • Organizer
      ENDO (The Endocrine Society) 2011
    • Place of Presentation
      Boston, USA.
    • Year and Date
      2011-06-06
  • [Presentation] 粘液型脂肪肉腫原因遺伝子TLS-CHOP 発現細胞の構築と SUMO-1 修飾の解析2010

    • Author(s)
      黒川 理樹
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      兵庫県兵庫市
    • Year and Date
      2010-12-09
  • [Presentation] shRNA とエネルギーエロクアンス2010

    • Author(s)
      黒川 理樹
    • Organizer
      第31回日本肥満学会
    • Place of Presentation
      群馬県前橋市
    • Year and Date
      2010-10-01
  • [Presentation] RNA 結合タンパク質 TLS/FUSによる非コード RNA 依存性転写抑制機構-筋萎縮性側索硬化症(ALS)の新規原因遺伝子 TLS/FUS-2010

    • Author(s)
      黒川 理樹
    • Organizer
      厚生労働科学研究費補助金 難治性疾患克服研究事業「筋萎縮性側索硬化症の病態に基づく画期的治療法の開発」班のワークショップ
    • Place of Presentation
      東京都千代田区
    • Year and Date
      2010-06-08
  • [Presentation] Noncoding RNA from cyclin D1 promoter regulates transcription via its binding to TLS, a novel causative gene for ALS.2010

    • Author(s)
      Kurokawa, R
    • Organizer
      独立行政法人理化学研究所脳科学総合研究センターセミナー
    • Place of Presentation
      埼玉県和光市
    • Year and Date
      2010-04-15
  • [Book] Generation of Functional Long Noncoding RNA Through Transcription and Natural Selection.2012

    • Author(s)
      Kurokawa, R
    • Total Pages
      151-174
    • Publisher
      Springer Berlin Heidelberg
  • [Book] Promoter-Associated Noncoding RNA from the CCND1 Promoter. In: Vancura, A. (ed) Transcriptional Regulation: Methods and Protocols, Methods in Molecular Biology 809, Chapter 392012

    • Author(s)
      Song, X., Wang, X., Arai, S., and Kurokawa, R.
    • Total Pages
      609-622
    • Publisher
      Springer New York
  • [Book] Promoter-Associated Long Noncoding RNAs Repress Transcription Through a RNA Binding Protein TLS. In: Collins, L.J. (ed) RNA Infrastructure and Networks: Advances in Experimental Medicine and Biology 722, Chapter 122011

    • Author(s)
      Kurokawa, R.
    • Total Pages
      196-208
    • Publisher
      Springer New York
  • [Remarks]

    • URL

      http://www.saitama-med.ac.jp/genome/

  • [Patent(Industrial Property Rights)] 抗 TLS モノクローナル抗体及びその製造方法、ハイブリドーマ及びその製造方法、並びに抗 TLS モノクローナル抗体含有組成物2013

    • Inventor(s)
      黒川 理樹
    • Industrial Property Rights Holder
      黒川 理樹
    • Industrial Property Number
      特願 2013-099962
    • Filing Date
      2013-05-10

URL: 

Published: 2014-08-29  

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