Live-cell imaging of HIV replication component trafficking and assembly

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390091
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Kitasato University
• Principal Investigator: MORIKAWA Yuko 北里大学, 大学院・感染制御科学府, 教授 (20191017)
• Project Period (FY): 2010 – 2012
• Keywords: 分子イメージング / 胞内輸送 / アセンブリー / 宿主因子 / リアルタイム

Research Abstract

Live-cell imaging revealed that Gag and GagPol proteins moved together with the transition from “confined” to “diffusive” and ”linear” movements. The linear movement was microtubule-based. The Gag/GagPol with the diffusive and linear movements were partially colocalized with some Rab and SNARE proteins. The RNA containing the Psi site, the signal for genome RNA packaging but not the RNA lacking the signal, was partially merged to Gag/GagPol. In the latter case, Gag/GagPol did not show a long linear movement.

Research Products

• [Journal Article] Trans-packaging of human immunodeficiency virus type 1 genome into Gag virus-like particles in Saccharomyces cerevisiae (2013)
  • Author(s): Tomo N, Goto T, Morikawa Y
  • Journal Title: Microb. Cell Fact Volume: 12 Pages: 28
  • DOI: DOI:10.1186/1475-2859-12-28
  • Peer Reviewed
• [Journal Article] Efavirenz enhances HIV-1 Gag processing at the plasma membrane through Gag-Pol dimerization (2013)
  • Author(s): Sudo S, Haraguchi H, Hirai Y, Gatanaga H, Sakuragi J-I, Momose F, Morikawa Y
  • Journal Title: J Virol Volume: 87 Pages: 3348-3360
  • DOI: DOI:10.1128/JVI.02306-12
  • Peer Reviewed
• [Journal Article] A large extension to HIV-1 Gag, like Pol, has negative impacts on virion assembly (2012)
  • Author(s): Haraguchi H, Noda T, Kawaoka Y, Morikawa Y
  • Journal Title: PLOS One Volume: 7 Pages: e47828
  • DOI: DOI:10.1371/journal.pone.004782
  • Peer Reviewed
• [Journal Article] HIV-1 infection ex vivoaccelerates measles virus infection by upregulating signaling lymphocytic activation molecule (SLAM) in CD4+T cells (2012)
  • Author(s): Mitsuki Y, Terahara K, Shibusawa K, Yamamoto T, Tsuchiya T, Ishige M, Kobayashi K, Morikawa Y, Nakayama T, Takeda M, Yanagi Y, Tsunetsugu-Yokota Y
  • Journal Title: J Virol Volume: 86 Pages: 7227-7234
  • DOI: DOI:10.1128/JVI.06681-11
  • Peer Reviewed
• [Journal Article] Novel postentry inhibitor of human immunodeficiency virus type 1 replication screened by yeast membrane-associated two-hybrid system (2011)
  • Author(s): Urano E, Kuramochi N, Ichikawa R, Yamagata Murayama S, Miyauchi K, Tomoda H, Takebe Y, Nermut M, Komano J, Morikawa Y
  • Journal Title: Antimicrob Agents Chemth Volume: 55 Pages: 4251-4260
  • DOI: DOI:10.1128/AAC.00299-11
  • Peer Reviewed
• [Journal Article] The cellular factors Vps18 and Mon2 are required for efficient production of infectious HIV-1 particles (2011)
  • Author(s): Tomita Y, Noda T, Fujii K, Morikawa Y, Kawaoka Y
  • Journal Title: J Virol Volume: 85 Pages: 5618-5627
  • DOI: DOI:10.1128/JVI.00846-10
  • Peer Reviewed
• [Journal Article] Huwe1, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity (2011)
  • Author(s): Yamamoto SP, Okawa K, Nakano T, Sano K, Ogawa K, Masuda T, Morikawa Y, Koyanagi Y, Suzuki Y
  • Journal Title: Mcirobes Infect Volume: 13 Pages: 339-349
  • DOI: DOI:10.1016/j.micinf.2010.12.002
  • Peer Reviewed
• [Journal Article] T cell-based functional cDNA library screening identified SEC13-like 1a carboxy-terminal domain as a negative regulator of human immunodeficiency virus replication (2010)
  • Author(s): Urano E, Ichikawa R, Morikawa Y, Yoshida T, Koyanagi Y, Komano J
  • Journal Title: Vaccine Volume: 28 Pages: B68-B73
  • DOI: DOI:10.1016/j.vaccine.2009.07.084
• [Journal Article] Intracellular localization of human immunodeficiency virus type 1 Gag and GagPol products and virus particle release: Relationship with the Gag-to-GagPol ratio (2010)
  • Author(s): Haraguchi H, Sudo S, Noda T, Momose F, Kawaoka Y, Morikawa Y
  • Journal Title: Microbiol Immunol Volume: 54 Pages: 734-746
  • DOI: DOI:10.1111/j.1348-0421.2010.00276.x
  • Peer Reviewed
• [Presentation] 生細胞分子イメージングによるHIV-1 Gag/GagPol蛋白の細胞内動態解析 (2012)
  • Author(s): 原口日和、森川裕子
  • Organizer: 第60回日本ウイルス学会学術集会.第60回日本ウイルス学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-13
• [Presentation] ヒト化マウスの麻疹ウイルスベクター評価系への応用 (2012)
  • Author(s): 池野翔太、寺原和孝、石毛真行、鈴木基臣、光木裕也、森川裕子、中山哲夫、横田恭子
  • Organizer: 第60回日本ウイルス学会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-13
• [Presentation] Efavirenz enhances HIV-1 Gag processing on the plasma membrane through GagPol dimerization (2012)
  • Author(s): Sudo S, Haraguchi H, Hirai Y, Gatanaga H, Sakuragi J-I, Momose F, Morikawa Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: 米国NY
  • Year and Date: 2012-05-23
• [Presentation] Rab蛋白質を指標としたHIV Gag蛋白質の細胞内輸送経路の解析 (2011)
  • Author(s): 中山順之、百瀬文隆、森川裕子
  • Organizer: 第34回日本分子生物学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 20111215-16
• [Presentation] HIV infection enhances the susceptibility of T cells to measles virus infection by upregulating signaling lymphocyte activation molecule (SLAM) expression (2011)
  • Author(s): Mitsuki Y, Shibusawa K, Terahara K, Kobayashi K, Morikawa Y, Nakayama T, Takeda M, Yanagi Y, Tsunetsugu Yokota Y
  • Organizer: 第15回国際ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-15
• [Presentation] The Pol region of human immunodeficiency virus GagPol negatively regulates its membrane binding and particle assembly (2011)
  • Author(s): Haraguchi H, Noda T, Kawaoka, Y, Morikawa Y
  • Organizer: 第15回国際ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-13
• [Presentation] Human immunodeficiency virus GagPol negatively regulates its membrane binding and particle assembly (2011)
  • Author(s): Haraguchi H, Noda T, Kawaoka, Y, Morikawa Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: 米国NY
  • Year and Date: 2011-05-24
• [Presentation] Inhibitor of HIV-1 Gag assembly screened by yeast membrane-associated system (2010)
  • Author(s): Urano E, Kuramochi N, Tomoda H, Takebe Y, Miyauchi K, Komano J, Morikawa Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: 米国NY
  • Year and Date: 20100500
• [Presentation] Modulation of HIV-1 infection at late phase by an integrase-interactor Huwe1 (2010)
  • Author(s): Yamamoto SP, Okawa K, Masuda T, Morikawa Y, Koyanagi Y, Suzuki Y
  • Organizer: CSH Retrovirus Meeting
  • Place of Presentation: 米国NY
  • Year and Date: 20100500
• [Presentation] Live-cell imaging of human immunodeficiency virus Gag and Gag-Pol trafficking (2010)
  • Author(s): Haraguchi H, Morikawa Y
  • Organizer: 第10回感染症免疫フォーラム
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Presentation] Overexpression of HRS induces BST-2 downregulation through its clathrin-binding domain (2010)
  • Author(s): Hoshino Y, Okunaga H, Morikawa Y
  • Organizer: 第10回感染症免疫フォーラム
  • Place of Presentation: 淡路島
  • Year and Date: 2010-09-09
• [Remarks]
  • URL: http://www.kitasato-u.ac.jp/lisci/life/chart/LSI-lab12.html
• [Remarks]
  • URL: http://www.kitasato-u.ac.jp/lisci/labo/ViralInfection2/index.html