2012 Fiscal Year Final Research Report
Mode of severe respiratory failure by SARS-CoV
| Project/Area Number |
22390094
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Research Institute, International Medical Center of Japan |
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Principal Investigator |
ISHIZAKA Yukihito 独立行政法人国立国際医療研究センター, 難治性疾患研究部, 部長 (90184514)
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| Co-Investigator(Renkei-kenkyūsha) |
嶋田 耕育 国立国際医療研究センター (50634185)
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| Project Period (FY) |
2010 – 2012
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| Keywords | SARS-CoV / 細胞内シグナル / 炎症 / PAI-1 |
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| Research Abstract |
Mode of sever respiratory symptoms induced by severe acute respiratory syndrome-corona virus (SARS-CoV) was investigated. When a psuedotype virus that expressed spike protein of SARS-CoV was infected to cells with angiotensin converting enzyme 2 (ACE2), a receptor of the virus, cellular signals involving MUC5AC, TGF-β and plasminogen activator inhibitor-1 were evoked. In contrast, a psuedotype virus that expressed spike protein of NL63-CoV, which was identified as a less-virulent CoV and was proved to infect cells via ACE2, did not. Next issue is to identify the intracellular domain of ACE2 and cellular proteins, which are responsible for the SARS-CoV induced gene expression.
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