2012 Fiscal Year Final Research Report
Establishment and application of the strategies for the discovery of biomarker proteins and peptides including species bound to albumin in serum/plasma
| Project/Area Number |
22390117
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
KODERA Yoshio 北里大学, 理学部物理学科, 准教授 (60265733)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Yuichi 北里大学, 医療衛生学部医療検査学科, 教授 (30178793)
MATSUMOTO Kazumasa 北里大学, 医学部泌尿器科, 講師 (70306603)
IWAMURA Masatsugu 北里大学, 医学部泌尿器科, 教授 (20176564)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEDA Tadakazu 北里大学, 名誉教授 (90265728)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 臨床検査医学 / 血中診断マーカー |
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| Research Abstract |
We developed a differential solubilization (DS) method to extract low-molecular-weight proteins/peptides containing many peptides bound to carrier proteins such as albumin in serum with good reproducibility and yield. Using the DS method we performed high-quality comparative analyses of more than 1500 peptides from 1μL of serum samples. Furthermore, we optimized the DS method to selectively enrich peptides less than 6 kDa. The modified DS method was combined with LC-MS and Two candidate peptides of renal cell carcinoma were discovered.
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[Journal Article] Establishment and application of a high-quality comparative analysis strategy for the discovery and small-scale validation of low-abundance biomarker peptides in serum based on an optimized novel peptide extraction method2013
Author(s)
Saito T, Kawashima Y, Minamida M, Matsumoto K, Araki K, Matsui T, SatohM, Nomura F, Iwamura M, Maeda M, BabaS, Kodera Y
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Journal Title
J. Electrophoresis
Volume: 57
Pages: 1-9
DOI
Peer Reviewed
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[Journal Article] Application of quantitative proteomic analysis using tandem mass tags for discovery and identification of novel biomarkers in periodontal disease2013
Author(s)
Tsuchida S, Satoh M, Kawashima Y, Sogawa K, Kado S, Sawai S, Nishimura M, Ogita M, Takeuchi Y, Kobyashi H, Aoki A, Kodera Y, Matsushita K, IzumiY, Nomura F
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Journal Title
Proteomics
Volume: (in press)
Peer Reviewed
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[Journal Article] Medaka fish, Oryzias latipes, as a model for human obesity-related glomerulopathy2013
Author(s)
Ichimura K, Kawashima Y, Nakamura T, Powell R, Hidoh Y, Terai S, Sakaida I,Kodera Y, Tsuji T, Ma JX, Sakai T, Matsumoto H, Obara T
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Journal Title
Biochem Biophys Res Commun
Volume: 431(4)
Pages: 712-7
Peer Reviewed
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[Journal Article] Integrated view of the human chromosome X-centric proteome project2013
Author(s)
Yamamoto T, Nakayama K, Hirano H,Tomonaga T, Ishihama Y, Yamada T, Kondo T, Kodera Y, Sato Y, Araki N, Mamitsuka H, Goshima N
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Journal Title
J Proteome Res
Volume: 12(1)
Pages: 58-61
Peer Reviewed
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[Journal Article] A strategy for large-scale phosphoproteomics and SRM-based validation of human breast cancer tissue samples2012
Author(s)
Narumi R, Murakami T, Kuga T, Adachi J, Shiromizu T, Muraoka S, Kume H, Kodera Y, Matsumoto M, Nakayama K, Miyamoto Y, Ishitobi M, Inaji H, Kato K, Tomonaga T
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Journal Title
J Proteome Res
Volume: 11(11)
Pages: 5311-22
Peer Reviewed
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[Journal Article] Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples2012
Author(s)
Muraoka S, Kume H, Watanabe S, Adachi J, Kuwano M, Sato M, Kawasaki N, Kodera Y, Ishitobi M, Inaji H, Miyamoto Y, Kato K, Tomonaga T
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Journal Title
J Proteome Res
Volume: 11(8)
Pages: 4201-10
Peer Reviewed
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[Journal Article] Proteomic analysis of gingival crevicular fluid for discovery of novel periodontal disease markers2012
Author(s)
Tsuchida S, Satoh M, Umemura H, Sogawa K, Kawashima Y, Kado S, Sawai S,Nishimura M, Kodera Y, Matsushita K, Nomura F
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Journal Title
Proteomics
Volume: 12(13)
Pages: 2190-202
Peer Reviewed
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