Forensic development of neuropathologicalanalyses for cerebrovascular diseases using ischemia model neuron

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390141
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: University of Yamanashi
• Principal Investigator: SHOJO Hideki 山梨大学, 大学院・医学工学総合研究部, 准教授 (60284626)
• Co-Investigator(Renkei-kenkyūsha): ADACHI Noboru 山梨大学, 大学院・医学工学総合研究部, 教授 (60282125) ; MABUCHI Tadashi 山梨大学, 大学院・医学工学総合研究部, 助教 (80150308)
• Project Period (FY): 2010 – 2012
• Keywords: 脳虚血

Research Abstract

In this study, we analyzed the neuropathological changes in cerebrovascular diseases and focused on the biomarker dynamics in order to understand a proper cause of death. Under ischemic conditions, we observed retrograde neurodegeneration, dendritic atrophy, and somatic swelling. Neuronal viability and respiration activity were significantly decreased in a dependent manner. These observations indicate that hypoxic conditions such as in ischemia, exacerbates the brain activity and the organelle function, especially in the mitochondria in which the morphology changed in the altered fixative conditions. These results demonstrate that observing the mitochondrial morphological changes in relation to the cause of death (e.g., sub- and acute death) would be a useful forensic diagnosis biomarker.

Research Products

• [Journal Article] Genetic and histologic evidence implicates role of inflammation in traumatic brain injury-induced apoptosis in the rat cerebral cortex following moderate fluid percussion injury (2010)
  • Author(s): Shojo H, Kaneko Y, Mabuchi T, Kibayashi K, Adachi N, Borlongan CV
  • Journal Title: Neuroscience Volume: 171 Pages: 1273-1282
  • Peer Reviewed
• [Journal Article] Acute treatment with herbal extracts provides neuroprotective benefits in in vitro and in vivo stroke models, characterized by reduced ischemic cell death and maintenance of motor and neurological functions (2010)
  • Author(s): Kaneko Y, Eve DJ, Yu SJ, Shojo H, Bae EC, Park D-H, Roschek B, Alberte RS, Sanberg PR, Sanberg CD, Bickford PC, Borlongan CV
  • Journal Title: Cell Med Volume: 1 Pages: 137-142
  • Peer Reviewed
• [Presentation] 頭部外傷におけるシクロオキシゲナーゼの分子動態の解析 (2012)
  • Author(s): 猩々英紀,安達登,馬淵正
  • Organizer: 第96 次日本法医学会総会
  • Place of Presentation: 浜松
  • Year and Date: 2012-06-09
• [Presentation] DJ-1 ameliorates neuronal cell death in ischemic stroke via mitochondrial pathway (2010)
  • Author(s): Tajiri N, Kaneko Y, Shojo H, Yu S, Bea E, Borlongan CV
  • Organizer: Society for Neuroscience
  • Place of Presentation: San Diego. U.S.A.
  • Year and Date: 20101113-17
• [Presentation] Traumatic brain injury-induced apoptosis in the rat cerebral cortex following moderate fluid percussion injury: A target for therapeutic intervention (2010)
  • Author(s): Shojo H, Kaneko Y, Adachi N, Borlongan CV
  • Organizer: 17thAnnual Conference of the American Society for Neural Therapy & Repair, Cell Transplant
  • Place of Presentation: Florida. U.S.A.
  • Year and Date: 20100429-0501
• [Presentation] Acute treatment of herbal extracts reduces ischemic cell death and improves motor and neurological functions in stroke models. (2010)
  • Author(s): Borlongan CV, Yu S, KanekoY, Shojo H, Bae E, Park DH, Eve DJ, Roschek, BJ, Alberte RS, Sanberg PR, Sanberg CD, Bickford PC
  • Organizer: 17thAnnual Conference of the American Society for Neural Therapy & Repair, Cell Transplant
  • Place of Presentation: Florida. U.S.A.
  • Year and Date: 20100429-0501
• [Presentation] 脳挫傷モデルラットを用いた頭部外傷の脳病態解析 (2010)
  • Author(s): 猩々英紀,安達登,馬淵正
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] 脳挫傷モデルラットを用いた頭部外傷後に引き起こされるアポトーシスの解析 (2010)
  • Author(s): 猩々英紀,安達登,馬淵正
  • Organizer: 第95 次日本法医学会総会
  • Place of Presentation: 福島
  • Year and Date: 2010-06-07
• [Presentation] a key neuroprotective protein against oxidative stress, ameliorates ischemic cell death in vitro possibly via mitochondrial pathway (2010)
  • Author(s): Kaneko Y, Shojo H, Yu S, Bae E, Borlongan CV. DJ-1
  • Organizer: Nano-Bio Collaborative Conference 2010
  • Place of Presentation: Florida. U.S.A.
  • Year and Date: 2010-03-11
• [Presentation] a key neuroprotective protein against Parkinson's disease, ameliorates neuronal cell death in ischemic stroke possibly via mitochondrial pathway (2010)
  • Author(s): Kaneko Y, Shojo H, Yu S, Bae E, Borlongan CV. DJ-1
  • Organizer: 20thUSF Health Research Day
  • Place of Presentation: Florida. U.S.A.
  • Year and Date: 2010-02-19
• [Presentation] National Incidence and Prevalence of Adult-Onset Brain Disorders (2010)
  • Author(s): Borlongan CV, Yu S, Recio J, Burns J, Amatangelo S, Bae E, Shojo H, Sanberg PR, Kaneko Y, Emerich DF
  • Organizer: 20th USF Health Research Day
  • Place of Presentation: Florida. U.S.A.
  • Year and Date: 2010-02-19
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/social/legal0me/classroom_member/shohjoh.html
• [Remarks]
  • URL: http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar/3_61/F8AD215DD10A64A4.html